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E4SN66

- E4SN66_LACAR

UniProt

E4SN66 - E4SN66_LACAR

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Protein

Spermidine/putrescine import ATP-binding protein PotA

Gene

potA

Organism
Lactobacillus amylovorus (strain GRL 1112)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.UniRule annotationSAAS annotation

Catalytic activityi

ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).UniRule annotationSAAS annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 448ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. putrescine-importing ATPase activity Source: InterPro
  3. spermidine-importing ATPase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotation

Keywords - Biological processi

TransportUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciLAMY695560:GI0Z-768-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine import ATP-binding protein PotAUniRule annotation (EC:3.6.3.31UniRule annotation)
Gene namesi
Name:potAUniRule annotation
Ordered Locus Names:LA2_03675Imported
OrganismiLactobacillus amylovorus (strain GRL 1112)Imported
Taxonomic identifieri695560 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000007033: Chromosome

Subcellular locationi

Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

  1. ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membraneUniRule annotationSAAS annotation, Membrane

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 235232ABC transporterUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily.UniRule annotation
Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation
Contains ABC transporter domain.SAAS annotation

Phylogenomic databases

KOiK11072.
OMAiYDEPVND.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR005893. Sp_pt_ABC_ATP-bd.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01187. potA. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E4SN66-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDIIKLKHIT KQYDDGFVAL KDINLTIESG KFYSLLGPSG SGKSTILRII
60 70 80 90 100
AGFTQPTDGK VYFDGKDITN LDAAKRHINT VFQNYALFPH LNVFENVAFG
110 120 130 140 150
LKIKKKPMNE IKPAVKDALH MVQLDGYANR EISELSGGQQ QRVAIARAIV
160 170 180 190 200
NEPKVLLLDE SLSALDKRLR KDMQFELRAI QKKLGITFIF VTHDQEEALS
210 220 230 240 250
MSDEIFVLND GEIQQSGSPV AIYDEPVNDF VARFIGDSNI LSGRMIKDYE
260 270 280 290 300
VEFANNKFKC ADGGIKPNEK VEVVIRPEDL DIVDPEKGKI VVTAQTQLFL
310 320 330 340 350
GDHFEIKAID ADENEWLIHS TNGVKLGQRI GLFFDPEDIH VMRLNESQAD
360
FDARIEKYEG DENED
Length:365
Mass (Da):41,108
Last modified:February 8, 2011 - v1
Checksum:i34ED46349EF16FA0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002338 Genomic DNA. Translation: ADQ58713.1.
RefSeqiYP_004031508.1. NC_014724.1.

Genome annotation databases

EnsemblBacteriaiADQ58713; ADQ58713; LA2_03675.
GeneIDi10022753.
KEGGilam:LA2_03675.
PATRICi43076473. VBILacAmy150400_0732.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002338 Genomic DNA. Translation: ADQ58713.1 .
RefSeqi YP_004031508.1. NC_014724.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADQ58713 ; ADQ58713 ; LA2_03675 .
GeneIDi 10022753.
KEGGi lam:LA2_03675.
PATRICi 43076473. VBILacAmy150400_0732.

Phylogenomic databases

KOi K11072.
OMAi YDEPVND.

Enzyme and pathway databases

BioCyci LAMY695560:GI0Z-768-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR005893. Sp_pt_ABC_ATP-bd.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view ]
Pfami PF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01187. potA. 1 hit.
PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Lactobacillus amylovorus GRL1112."
    Kant R., Paulin L., Alatalo E., de Vos W.M., Palva A.
    J. Bacteriol. 193:789-790(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GRL 1112Imported.

Entry informationi

Entry nameiE4SN66_LACAR
AccessioniPrimary (citable) accession number: E4SN66
Entry historyi
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: October 29, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3