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Protein

Ribonuclease 3

Gene

rnc

Organism
Lactobacillus amylovorus (strain GRL 1112)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491MagnesiumUniRule annotation
Active sitei53 – 531UniRule annotation
Metal bindingi122 – 1221MagnesiumUniRule annotation
Metal bindingi125 – 1251MagnesiumUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseUniRule annotationSAAS annotation, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processingUniRule annotationSAAS annotation, rRNA processingUniRule annotationSAAS annotation, tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, RNA-binding, rRNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciLAMY695560:GI0Z-1445-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotationSAAS annotation (EC:3.1.26.3UniRule annotationSAAS annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Gene namesi
Name:rncUniRule annotationImported
Ordered Locus Names:LA2_07330Imported
OrganismiLactobacillus amylovorus (strain GRL 1112)Imported
Taxonomic identifieri695560 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000007033: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 136130RNase IIIUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.UniRule annotation
Contains 1 RNase III domain.UniRule annotation
Contains DRBM (double-stranded RNA-binding) domain.SAAS annotation
Contains RNase III domain.SAAS annotation

Phylogenomic databases

HOGENOMiHOG000246808.
KOiK03685.
OMAiSADHNER.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS00517. RNASE_3_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E4SKB4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVSTKFLKKL KDEYNVKFNN EKLLEEAFTH SSYSNEHPDA GIRDYEKLEF
60 70 80 90 100
LGDAVLELAV SNYLYRHYPK LNEGELTRLR SNIVRTEGFS EFAIECGFQK
110 120 130 140 150
EIHLGNGEEK AGARNRKTLL EDVFEAFNGA LYLDQGMPAV EHFLHLTVYP
160 170 180 190 200
LIDKGEFDDS RDYKTDLQEL LQQNGPVNIE YSVISESQLP SHFEVELKIN
210 220
DEAKTRGEGH NKKAAEQQAA KAALKELE
Length:228
Mass (Da):26,091
Last modified:February 8, 2011 - v1
Checksum:iB0FC04BB6088C991
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002338 Genomic DNA. Translation: ADQ59387.1.
RefSeqiYP_004032182.1. NC_014724.1.

Genome annotation databases

EnsemblBacteriaiADQ59387; ADQ59387; LA2_07330.
GeneIDi10023430.
KEGGilam:LA2_07330.
PATRICi43077910. VBILacAmy150400_1445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002338 Genomic DNA. Translation: ADQ59387.1.
RefSeqiYP_004032182.1. NC_014724.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADQ59387; ADQ59387; LA2_07330.
GeneIDi10023430.
KEGGilam:LA2_07330.
PATRICi43077910. VBILacAmy150400_1445.

Phylogenomic databases

HOGENOMiHOG000246808.
KOiK03685.
OMAiSADHNER.

Enzyme and pathway databases

BioCyciLAMY695560:GI0Z-1445-MONOMER.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS00517. RNASE_3_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Lactobacillus amylovorus GRL1112."
    Kant R., Paulin L., Alatalo E., de Vos W.M., Palva A.
    J. Bacteriol. 193:789-790(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GRL 1112Imported.

Entry informationi

Entry nameiE4SKB4_LACAR
AccessioniPrimary (citable) accession number: E4SKB4
Entry historyi
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: February 4, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.