E4RZ68 (E4RZ68_LEAB4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
D-alanine--D-alanine ligase EMBL ADQ19186.1
EC=6.3.2.4 EMBL ADQ19186.1

Gene names

Ordered Locus Names:

Lbys_3538

Organism

Leadbetterella byssophila (strain DSM 17132 / KACC 11308 / 4M15) [Complete proteome] [HAMAP] EMBL ADQ19186.1

Taxonomic identifier

649349 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Cytophagia › Cytophagales › Cytophagaceae › Leadbetterella › Leadbetterella byssophila

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. SAAS SAAS005905
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905
Subcellular location	Cytoplasm By similarity SAAS SAAS005905.
Sequence similarities	Contains 1 ATP-grasp domain. SAAS SAAS005905

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation SAAS SAAS005905 Peptidoglycan synthesis SAAS SAAS005905
Cellular component	Cytoplasm SAAS SAAS005905
Ligand	ATP-binding SAAS SAAS005905 Magnesium SAAS SAAS005905 Manganese SAAS SAAS005905 Metal-binding SAAS SAAS005905 Nucleotide-binding
Molecular function	Ligase SAAS SAAS005905 EMBL ADQ19186.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	E4RZ68 [UniParc]. Last modified February 8, 2011. Version 1. Checksum: CC0EBA8B0E9620C7	FASTA	585	66,307
	10 20 30 40 50 60 MDKKIKVGIF FGGQSREREI SFLGGRTAYE HLDKRFFEPV LIFVDSLGNF IHVEPSILNE 70 80 90 100 110 120 ADIRSFYPSK NQNNGYKVYI ESLGPLKVSQ LYKLIYKIGS QVKIEELKSY MDFAFVVMHG 130 140 150 160 170 180 PSAEDGSIQG LLEWLNIPYL GPGILGSAVG INKPFQNKLM AYATGQEKKY LTISKREWNT 190 200 210 220 230 240 SDRSKLFSSV IAKLGFPFVV KAPHQGSSIG VAIVRKRSLE EFSKAMSQCF FETFITKKEW 250 260 270 280 290 300 DKLSPRRKKN VMEKMSNLDE GIGFPVVLDG EQIYHPATLL KKLDEYLTQH EVAQLNSVNG 310 320 330 340 350 360 EDYVLIEEFI SGQEFSCGVI QDDEGRVLPL PPAEVYGETA TFDFKAKYKS TVSKKRIPVK 370 380 390 400 410 420 TSDANLEKIH EDVLKAFEAT GMSVICRIDG FITEDDVVVL HDPNTLPGMS PSSFIFKQMA 430 440 450 460 470 480 EVGFNITNSL TYFIRQSLIE RIRSGKNQFE VQQLLNHLST QIKNAPARKR LAVLFGENEK 490 500 510 520 530 540 EFILAKKVYN ELCGSEEYEP VPVCAALNGS RYIIPLNLMY KPDINEFGTA IATPKHPFIQ 550 560 570 580 KNIELVKQVR AFYAGEVDFS VRKVSEQDYN QIFKHTYDCL TGELH

References

[1]

"The complete genome of Leadbetterella byssophila DSM 17132." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Teshima H., Brettin T., Detter J.C., Han C., Tapia R., Land M. , Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Tindall B., Pomrenke H.G., Brambilla E., Klenk H.-P., Eisen J.A. Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17132.

[2]

"Complete genome sequence of Leadbetterella byssophila type strain (4M15)." Abt B., Tashima H., Lucas S., Lapidus A., Glavina Del Rio T., Nolan M., Tice H., Cheng J., Pitluck S., Liolios K., Pagani I., Ivanova N., Mavromatis K., Pati A., Tapia R., Han C., Goodwin L., Chen A. , Palaniappan K., Land M., Hauser L., Chang Y., Jeffries C., Rohde M., Goker M., Tindall B., Detter J., Woyke T., Bristow J., Eisen J., Markowitz V., Hugenholtz P., Klenk H., Kyrpides N. Stand. Genomic Sci. 4:2-12(2011) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17132 / KACC 11308 / 4M15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002305 Genomic DNA. Translation: ADQ19186.1.
RefSeq	YP_003999539.1. NC_014655.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADQ19186; ADQ19186; Lbys_3538.
GeneID	9957424.
KEGG	lby:Lbys_3538.
PATRIC	42657207. VBILeaBys116579_3633.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000011592.
KO	K01921.
OMA	IQGLLEW.
Enzyme and pathway databases
BioCyc	LBYS649349:GHFA-3590-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 2 hits. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4RZ68_LEAB4

Accession

Primary (citable) accession number: E4RZ68

Entry history

Integrated into UniProtKB/TrEMBL:	February 8, 2011
Last sequence update:	February 8, 2011
Last modified:	May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)