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E4RIH0 (E4RIH0_HALHG) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Halsa_1615 EMBL ADQ15040.1
OrganismHalanaerobium hydrogeniformans (Halanaerobium sp. (strain sapolanicus)) [Complete proteome] [HAMAP] EMBL ADQ15040.1
Taxonomic identifier656519 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalanaerobium

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011127

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011127

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011127

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011127

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011127.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011127

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain135 – 342208ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2961Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3091Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3091Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3111Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E4RIH0 [UniParc].

Last modified February 8, 2011. Version 1.
Checksum: 7484E943E2337AC6

FASTA34939,875
        10         20         30         40         50         60 
MSSSKINIAL IYGGRSAEHE VSILSTRSIY NNLDKSKYNI FPIAISKTGR WFETEKSLEY 

        70         80         90        100        110        120 
LNSETNSIPD QADRKLFPLE VIDFLQKNID LAFPVLHGPY GEDGKIQGFF EILDIPYVGC 

       130        140        150        160        170        180 
NLSSSAVSMD KELMKKLFAY HDIPQTKFQV LKKSQYKVKR KKEFYEELTK KLGLPFFVKP 

       190        200        210        220        230        240 
ANMGSSIGIT KVNEKKNFAE AINSAFEHDN KIIIENFVKA REIEASIISL DNEIRVSIPG 

       250        260        270        280        290        300 
EIIAQHDFYD YKAKYQDSKT RLVIPAEIDD KIKKEIKLLA VKAFNAVGGD ALARIDFFLT 

       310        320        330        340 
ENNDLLVNEI NTMPGFTQFS MYPLLFKESG FPYQKLLDQM IEQALNRDI 

« Hide

References

[1]"Complete sequence of Halanaerobium sp. sapolanicus."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N., Begemann M.B. expand/collapse author list , Mormile M.R., Wall J.D., Elias D.A., Woyke T.
Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: sapolanicus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002304 Genomic DNA. Translation: ADQ15040.1.
RefSeqYP_003995394.1. NC_014654.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADQ15040; ADQ15040; Halsa_1615.
GeneID9969234.
KEGGhas:Halsa_1615.
PATRIC42641925. VBIHalSp157090_1683.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycHHYD656519:GHYV-1648-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE4RIH0_HALHG
AccessionPrimary (citable) accession number: E4RIH0
Entry history
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: February 19, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)