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E4NDX1

- E4NDX1_KITSK

UniProt

E4NDX1 - E4NDX1_KITSK

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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Kitasatospora setae (strain ATCC 33774 / DSM 43861 / JCM 3304 / KCC A-0304 / NBRC 14216 / KM-6054) (Streptomyces setae)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Magnesium.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei312 – 3121Coenzyme AUniRule annotation
Binding sitei501 – 5011ATPUniRule annotation
Binding sitei516 – 5161ATPUniRule annotation
Binding sitei524 – 5241Coenzyme A; via carbonyl oxygenUniRule annotation
Metal bindingi538 – 5381Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi540 – 5401Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi543 – 5431Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi388 – 3903ATPUniRule annotation
Nucleotide bindingi412 – 4176ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciKSET452652:GJFD-3594-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotationImported
Ordered Locus Names:KSE_35980Imported
OrganismiKitasatospora setae (strain ATCC 33774 / DSM 43861 / JCM 3304 / KCC A-0304 / NBRC 14216 / KM-6054) (Streptomyces setae)Imported
Taxonomic identifieri452652 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeKitasatospora
ProteomesiUP000007076: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei610 – 6101N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliE4NDX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 1964Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

KOiK01895.
OMAiAWIWYRD.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E4NDX1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNESLANLL KEERRFAPPA ELAASANVTA DAYAQASEDR LAFWAEQARR
60 70 80 90 100
LSWAVEPTET LDWSNPPFAK WFADGKLNVA YNCVDRHVEA GNGDRVAIHF
110 120 130 140 150
EGEPGDSRAI TYAQLKDEVS KAANALESLG VAKGDRVAVY LPMIPEAVVA
160 170 180 190 200
MLACARIGAA HSVVFGGFSA DAVASRIKDA DAKLVITADG GYRRGKPSAL
210 220 230 240 250
KPAIDEALTK VDGVENVLVV RRTGEEVAWT EGRDVWWHEL VDGQSAEHTP
260 270 280 290 300
EAHDAEQPLF ILYTSGTTGK PKGILHTSGG YLTQASFTHN AVFDLKPETD
310 320 330 340 350
VYWCTADIGW VTGHSYIVYG PLSNGATQVI YEGTPDTPHQ GRMFEIVQKY
360 370 380 390 400
GVTILYTAPT LIRTWMKWGD DIPAGFDLSS LRVLGSVGEP INPEAWVWYR
410 420 430 440 450
EHIGAGKTPI VDTWWQTETG AIMISPLPGV TETKPGSAQR ALPGIAATVV
460 470 480 490 500
DDEAREVPNG SGGYLVLTEP WPSMLRTIWG DDERYVDTYW SRFEGRYFAG
510 520 530 540 550
DGAKKDEDGD IWLLGRVDDV MLVSGHNIST TEVESALVGH PAVAESAVVG
560 570 580 590 600
AADATTGQAI VAFVILRGTA ADSEELIADL RNHVGKTLGP IAKPKQIKVV
610 620 630 640 650
SELPKTRSGK IMRRLLRDIA EGREVGDTTT LADSSVMNLI QSQLPAAGAE

G
Length:651
Mass (Da):70,350
Last modified:February 8, 2011 - v1
Checksum:i227EFC38369A670D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP010968 Genomic DNA. Translation: BAJ29402.1.
RefSeqiYP_004905358.1. NC_016109.1.

Genome annotation databases

EnsemblBacteriaiBAJ29402; BAJ29402; KSE_35980.
GeneIDi11348269.
KEGGiksk:KSE_35980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP010968 Genomic DNA. Translation: BAJ29402.1 .
RefSeqi YP_004905358.1. NC_016109.1.

3D structure databases

ProteinModelPortali E4NDX1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAJ29402 ; BAJ29402 ; KSE_35980 .
GeneIDi 11348269.
KEGGi ksk:KSE_35980.

Phylogenomic databases

KOi K01895.
OMAi AWIWYRD.

Enzyme and pathway databases

BioCyci KSET452652:GJFD-3594-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33774 / DSM 43861 / JCM 3304 / KCC A-0304 / NBRC 14216 / KM-6054Imported.

Entry informationi

Entry nameiE4NDX1_KITSK
AccessioniPrimary (citable) accession number: E4NDX1
Entry historyi
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: October 29, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3