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Protein

Alpha-humulene 10-hydroxylase

Gene

CYP71BA1

Organism
Zingiber zerumbet (Shampoo ginger) (Amomum zerumbet)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of alpha-humulene to 10-hydroxy-alpha-humulene in the second step of zerumbone biosynthesis, a predominating potential multi-anticancer agent.1 Publication

Miscellaneous

The recommended numbering of humulene gives 10-hydroxy-alpha-humulene as the product rather than 8-hydroxy-alpha-humulene as used by the literature.

Catalytic activityi

Alpha-humulene + O2 + NADPH = 10-hydroxy-alpha-humulene + NADP+ + H2O.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi452Iron (heme axial ligand)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.150. 12510.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-humulene 10-hydroxylase (EC:1.14.13.150)
Gene namesi
Name:CYP71BA1
OrganismiZingiber zerumbet (Shampoo ginger) (Amomum zerumbet)
Taxonomic identifieri311405 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaZingiberalesZingiberaceaeZingiber

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004187501 – 513Alpha-humulene 10-hydroxylaseAdd BLAST513

Expressioni

Tissue specificityi

Expressed at highest level in rhizomes and much less in leaves. Not expressed in stems.1 Publication

Structurei

3D structure databases

SMRiE3W9C4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

E3W9C4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAISLFSPF FFITLFLGFF ITLLIKRSSR SSVHKQQVLL ASLPPSPPRL
60 70 80 90 100
PLIGNIHQLV GGNPHRILLQ LARTHGPLIC LRLGQVDQVV ASSVEAVEEI
110 120 130 140 150
IKRHDLKFAD RPRDLTFSRI FFYDGNAVVM TPYGGEWKQM RKIYAMELLN
160 170 180 190 200
SRRVKSFAAI REDVARKLTG EIAHKAFAQT PVINLSEMVM SMINAIVIRV
210 220 230 240 250
AFGDKCKQQA YFLHLVKEAM SYVSSFSVAD MYPSLKFLDT LTGLKSKLEG
260 270 280 290 300
VHGKLDKVFD EIIAQRQAAL AAEQAEEDLI IDVLLKLKDE GNQEFPITYT
310 320 330 340 350
SVKAIVMEIF LAGTETSSSV IDWVMSELIK NPKAMEKVQK EMREAMQGKT
360 370 380 390 400
KLEESDIPKF SYLNLVIKET LRLHPPGPLL FPRECRETCE VMGYRVPAGA
410 420 430 440 450
RLLINAFALS RDEKYWGSDA ESFKPERFEG ISVDFKGSNF EFMPFGAGRR
460 470 480 490 500
ICPGMTFGIS SVEVALAHLL FHFDWQLPQG MKIEDLDMME VSGMSATRRS
510
PLLVLAKLII PLP
Length:513
Mass (Da):57,798
Last modified:February 8, 2011 - v1
Checksum:iD7DE5F757C46C25C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB331234 mRNA. Translation: BAJ39893.1.

Genome annotation databases

KEGGiag:BAJ39893.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB331234 mRNA. Translation: BAJ39893.1.

3D structure databases

SMRiE3W9C4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAJ39893.

Enzyme and pathway databases

BRENDAi1.14.13.150. 12510.

Family and domain databases

InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiC71A1_ZINZE
AccessioniPrimary (citable) accession number: E3W9C4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: February 8, 2011
Last modified: May 10, 2017
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.