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E3QW41

- MAP22_COLGM

UniProt

E3QW41 - MAP22_COLGM

Protein

Methionine aminopeptidase 2-2

Gene

GLRG_10223

Organism
Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei194 – 1941SubstrateUniRule annotation
    Metal bindingi214 – 2141Divalent metal cation 1UniRule annotation
    Metal bindingi225 – 2251Divalent metal cation 1UniRule annotation
    Metal bindingi225 – 2251Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi294 – 2941Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei302 – 3021SubstrateUniRule annotation
    Metal bindingi327 – 3271Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi421 – 4211Divalent metal cation 1UniRule annotation
    Metal bindingi421 – 4211Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-HAMAP
    2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 2-2UniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 2-2UniRule annotation
    Short name:
    MetAP 2-2UniRule annotation
    Alternative name(s):
    Peptidase MUniRule annotation
    Gene namesi
    ORF Names:GLRG_10223
    OrganismiColletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola)
    Taxonomic identifieri645133 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesGlomerellaceaemitosporic GlomerellaceaeColletotrichum
    ProteomesiUP000008782: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 440440Methionine aminopeptidase 2-2PRO_0000407605Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliE3QW41.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi57 – 6913Lys-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

    Phylogenomic databases

    OrthoDBiEOG7BGHW3.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPiMF_03175. MetAP_2_euk.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 2 hits.
    TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
    PROSITEiPS01202. MAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E3QW41-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAQVPTEAL KELNVGGPAT NDKAANPTEG NGVDHDSDDS DEEGEEVAAP    50
    AAGGAAKKKK KNKKKKKKKS PTAQSDPPRV LMSSLFPNKN YPKGQEEEYR 100
    DENLYRTTNE EKRHLDNLNN DFLTDYREAA EIHRQVRQWA QKNIKPGQTL 150
    TEIAEGIEDG VRALTGHPGI EEGDAYKGGM GFPCGLSLNH CAAHYTPNAG 200
    NKMVLSQGDV MKVDFGVHVN GRIVDSAFTM AFEPQYDNLL AAVKDATNAG 250
    VKEAGIDVRV GDVGGVIQEV MESYEVEIDG TTYPVKSIRN LNGHTIERWS 300
    IHGTKSVPIV KSNDTTKMEE GDVFAVETFG STGNGFVRED MEVSHYAKRG 350
    EGHAALRLDS AKRLLNVINK NFGTLPFCRR YLDRLGQDKY LLGLNNLVSS 400
    GIVEAYPPLC DKKGSYTAQF EHTILLRPTV KEVISRGDDY 440
    Length:440
    Mass (Da):48,286
    Last modified:January 11, 2011 - v1
    Checksum:i7303EE074317C86B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG697387 Genomic DNA. Translation: EFQ35079.1.

    Genome annotation databases

    EnsemblFungiiEFQ35079; EFQ35079; GLRG_10223.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG697387 Genomic DNA. Translation: EFQ35079.1 .

    3D structure databases

    ProteinModelPortali E3QW41.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFQ35079 ; EFQ35079 ; GLRG_10223 .

    Phylogenomic databases

    OrthoDBi EOG7BGHW3.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPi MF_03175. MetAP_2_euk.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10804:SF9. PTHR10804:SF9. 1 hit.
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 2 hits.
    TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
    PROSITEi PS01202. MAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses."
      O'Connell R.J., Thon M.R., Hacquard S., Amyotte S.G., Kleemann J., Torres M.F., Damm U., Buiate E.A., Epstein L., Alkan N., Altmueller J., Alvarado-Balderrama L., Bauser C.A., Becker C., Birren B.W., Chen Z., Choi J., Crouch J.A.
      , Duvick J.P., Farman M.A., Gan P., Heiman D., Henrissat B., Howard R.J., Kabbage M., Koch C., Kracher B., Kubo Y., Law A.D., Lebrun M.-H., Lee Y.-H., Miyara I., Moore N., Neumann U., Nordstroem K., Panaccione D.G., Panstruga R., Place M., Proctor R.H., Prusky D., Rech G., Reinhardt R., Rollins J.A., Rounsley S., Schardl C.L., Schwartz D.C., Shenoy N., Shirasu K., Sikhakolli U.R., Stueber K., Sukno S.A., Sweigard J.A., Takano Y., Takahara H., Trail F., van der Does H.C., Voll L.M., Will I., Young S., Zeng Q., Zhang J., Zhou S., Dickman M.B., Schulze-Lefert P., Ver Loren van Themaat E., Ma L.-J., Vaillancourt L.J.
      Nat. Genet. 44:1060-1065(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: M1.001 / M2 / FGSC 10212.

    Entry informationi

    Entry nameiMAP22_COLGM
    AccessioniPrimary (citable) accession number: E3QW41
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3