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E3QDQ4

- MAP21_COLGM

UniProt

E3QDQ4 - MAP21_COLGM

Protein

Methionine aminopeptidase 2-1

Gene

GLRG_04136

Organism
Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 23 (01 Oct 2014)
      Sequence version 1 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei214 – 2141SubstrateUniRule annotation
    Metal bindingi235 – 2351Divalent metal cation 1UniRule annotation
    Metal bindingi246 – 2461Divalent metal cation 1UniRule annotation
    Metal bindingi246 – 2461Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi315 – 3151Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei323 – 3231SubstrateUniRule annotation
    Metal bindingi348 – 3481Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi444 – 4441Divalent metal cation 1UniRule annotation
    Metal bindingi444 – 4441Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-HAMAP
    2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 2-1UniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 2-1UniRule annotation
    Short name:
    MetAP 2-1UniRule annotation
    Alternative name(s):
    Peptidase MUniRule annotation
    Gene namesi
    ORF Names:GLRG_04136
    OrganismiColletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola)
    Taxonomic identifieri645133 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesGlomerellaceaemitosporic GlomerellaceaeColletotrichum
    ProteomesiUP000008782: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 463463Methionine aminopeptidase 2-1PRO_0000407627Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliE3QDQ4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi68 – 725Poly-Lys
    Compositional biasi75 – 795Poly-Lys

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

    Phylogenomic databases

    OrthoDBiEOG7BGHW3.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPiMF_03175. MetAP_2_euk.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 2 hits.
    TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
    PROSITEiPS01202. MAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E3QDQ4-1 [UniParc]FASTAAdd to Basket

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    MGSKTPEEQI PGGNGGPPST GPSSSGGEPR GTHLSRDGDG SLGDHGDDDD    50
    ADEDDVSSRP LRADVEEKKK KKRPKKKKKP AAAKEQSSPP RVPLSDLFPL 100
    GEYPAGEDLV YDRVPQPDAN TARTTTAELR YQSRKHLEDP ALLNDYRKAA 150
    EVHRQVRHWV QEAVKPGWTL LDIATGIEDG VRSLLANQGI EPGDNLRSGM 200
    GFPTGLCLNH ETAHYTPNPG QRDVVLQHGD VMKVDYGVQV NGWIVDSAFT 250
    MSFDPTYDNL LAAARDATNS GIKAAGIDVR ICDVSAEIQE AMESYEVEIR 300
    GKTYPVKAVR NICAHDIKRY RIHGGKSIPF IRNNDQTKME EGEIFAIETF 350
    GTTGRGKLYD DIGVYGYGLL HDAPAQVRLP FASANRLCKT IKEQFGSIVF 400
    CRRYLDRLGL DRYLAGLNCL VSHGVLESYA PLADIKGSYT SQFEHTILLR 450
    ESSKEIVSRG SDY 463
    Length:463
    Mass (Da):50,912
    Last modified:January 11, 2011 - v1
    Checksum:i02C808D4B6DD28F2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG697343 Genomic DNA. Translation: EFQ28992.1.

    Genome annotation databases

    EnsemblFungiiEFQ28992; EFQ28992; GLRG_04136.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG697343 Genomic DNA. Translation: EFQ28992.1 .

    3D structure databases

    ProteinModelPortali E3QDQ4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFQ28992 ; EFQ28992 ; GLRG_04136 .

    Phylogenomic databases

    OrthoDBi EOG7BGHW3.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPi MF_03175. MetAP_2_euk.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 2 hits.
    TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
    PROSITEi PS01202. MAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses."
      O'Connell R.J., Thon M.R., Hacquard S., Amyotte S.G., Kleemann J., Torres M.F., Damm U., Buiate E.A., Epstein L., Alkan N., Altmueller J., Alvarado-Balderrama L., Bauser C.A., Becker C., Birren B.W., Chen Z., Choi J., Crouch J.A.
      , Duvick J.P., Farman M.A., Gan P., Heiman D., Henrissat B., Howard R.J., Kabbage M., Koch C., Kracher B., Kubo Y., Law A.D., Lebrun M.-H., Lee Y.-H., Miyara I., Moore N., Neumann U., Nordstroem K., Panaccione D.G., Panstruga R., Place M., Proctor R.H., Prusky D., Rech G., Reinhardt R., Rollins J.A., Rounsley S., Schardl C.L., Schwartz D.C., Shenoy N., Shirasu K., Sikhakolli U.R., Stueber K., Sukno S.A., Sweigard J.A., Takano Y., Takahara H., Trail F., van der Does H.C., Voll L.M., Will I., Young S., Zeng Q., Zhang J., Zhou S., Dickman M.B., Schulze-Lefert P., Ver Loren van Themaat E., Ma L.-J., Vaillancourt L.J.
      Nat. Genet. 44:1060-1065(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: M1.001 / M2 / FGSC 10212.

    Entry informationi

    Entry nameiMAP21_COLGM
    AccessioniPrimary (citable) accession number: E3QDQ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3