Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional lycopene cyclase/phytoene synthase

Gene

GLRG_02475

Organism
Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase).By similarity

Catalytic activityi

Carotenoid psi-end group = carotenoid beta-end group.
2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathwayi

GO - Molecular functioni

  1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB-EC
  2. intramolecular lyase activity Source: InterPro

GO - Biological processi

  1. carotenoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
UPA00802.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lycopene cyclase/phytoene synthase
Including the following 2 domains:
Lycopene beta-cyclase (EC:5.5.1.19)
Alternative name(s):
Lycopene cyclase
Phytoene synthase (EC:2.5.1.32)
Gene namesi
ORF Names:GLRG_02475
OrganismiColletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola)
Taxonomic identifieri645133 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesGlomerellaceaeColletotrichum
ProteomesiUP000008782: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821HelicalSequence AnalysisAdd
BLAST
Transmembranei35 – 5521HelicalSequence AnalysisAdd
BLAST
Transmembranei77 – 9721HelicalSequence AnalysisAdd
BLAST
Transmembranei120 – 14021HelicalSequence AnalysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence AnalysisAdd
BLAST
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST
Transmembranei220 – 24021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587Bifunctional lycopene cyclase/phytoene synthasePRO_0000409235Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliE3Q717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 242242Lycopene beta-cyclaseAdd
BLAST
Regioni249 – 587339Phytoene synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the lycopene beta-cyclase family.Curated
In the C-terminal section; belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiEOG7RBZJ2.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E3Q717-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYDYALVHV KYTIPLAALL TVFSYPVFTR LDVVRTLFIV TIAFVATIPW
60 70 80 90 100
DSYLIRTNVW TYPPDAVLGP TLYDIPAEEL FFFIIQTYIT AQLYIILNKP
110 120 130 140 150
VLHAQYLNSP ATLPQWIKSG KLVGQLALSG SVLLGTWLIA KKGEGTYLGL
160 170 180 190 200
ILVWACTFAL FTWTITAHFL LALPLACTAL PILLPTVYLW IVDEMALGRG
210 220 230 240 250
TWAIESGTKL ELQLFGSLEI EEATFFLVTN MLIVFGIAAF DKAVAVCDAF
260 270 280 290 300
PEKFDKPADA LAMSLLRARV FPSSKYDMQR ILGIRQAAAR LAKKSRSFHL
310 320 330 340 350
ASSVFPGRLR IDLTLLYSYC RLADDLVDDA ATPEEAAVWI SKLDRHLSLL
360 370 380 390 400
YKDPDATSTP LASKYAAENF PPSALSALDM LPTSLLPREP LAELLKGFEM
410 420 430 440 450
DLSFSNSAFP IADPEDLELY AARVASTVGQ ACLELVFCHC QHGLPDYMKA
460 470 480 490 500
YLRNTARQMG LALQFVNISR DIAVDAKIGR VYLPTTWLKE EGLTPEDVLK
510 520 530 540 550
SPNSEGVGKV RRRILAKALD HYGEARDSMK WIPSEARGPM IVAVESYMEI
560 570 580
GRVLMRNGGS AAADGSGRAT VPKSRRIWVA WSTLMAA
Length:587
Mass (Da):64,983
Last modified:January 11, 2011 - v1
Checksum:i6D39085251FBB666
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG697335 Genomic DNA. Translation: EFQ26655.1.

Genome annotation databases

EnsemblFungiiEFQ26655; EFQ26655; GLRG_02475.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG697335 Genomic DNA. Translation: EFQ26655.1.

3D structure databases

ProteinModelPortaliE3Q717.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFQ26655; EFQ26655; GLRG_02475.

Phylogenomic databases

OrthoDBiEOG7RBZJ2.

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
UPA00802.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses."
    O'Connell R.J., Thon M.R., Hacquard S., Amyotte S.G., Kleemann J., Torres M.F., Damm U., Buiate E.A., Epstein L., Alkan N., Altmueller J., Alvarado-Balderrama L., Bauser C.A., Becker C., Birren B.W., Chen Z., Choi J., Crouch J.A.
    , Duvick J.P., Farman M.A., Gan P., Heiman D., Henrissat B., Howard R.J., Kabbage M., Koch C., Kracher B., Kubo Y., Law A.D., Lebrun M.-H., Lee Y.-H., Miyara I., Moore N., Neumann U., Nordstroem K., Panaccione D.G., Panstruga R., Place M., Proctor R.H., Prusky D., Rech G., Reinhardt R., Rollins J.A., Rounsley S., Schardl C.L., Schwartz D.C., Shenoy N., Shirasu K., Sikhakolli U.R., Stueber K., Sukno S.A., Sweigard J.A., Takano Y., Takahara H., Trail F., van der Does H.C., Voll L.M., Will I., Young S., Zeng Q., Zhang J., Zhou S., Dickman M.B., Schulze-Lefert P., Ver Loren van Themaat E., Ma L.-J., Vaillancourt L.J.
    Nat. Genet. 44:1060-1065(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: M1.001 / M2 / FGSC 10212.

Entry informationi

Entry nameiLCPS_COLGM
AccessioniPrimary (citable) accession number: E3Q717
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: January 11, 2011
Last modified: March 4, 2015
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.