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E3PZS2 (STAD2_OPHSP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 11. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic
EC=1.14.19.2
Alternative name(s):
Stearoyl-ACP desaturase 2
Gene names
Name:SAD2
OrganismOphrys sphegodes (Early spider orchid) (Arachnites aranifera)
Taxonomic identifier145953 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Asparagales › Orchidaceae › Orchidoideae › Orchideae › Orchidinae › Ophrys

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons Delta9 and Delta10 of the acyl chain. Converts palmitoyl-ACP to hexadecenoic acid by introduction of a cis double bond between carbons Delta4 and Delta5 of the acyl chain. Catalyzes saturated fatty acid 18:0 and 16:0 to generate 18:1 (delta-9) and 16:1 (delta-4) intermediates, expected to give rise to 9-alkenes and 12-alkenes, respectively. Ref.1

Catalytic activity

Stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier-protein] + acceptor + 2 H2O. Ref.1

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Lipid metabolism; fatty acid metabolism.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastid › chloroplast stroma By similarity.

Tissue specificity

Preferentially expressed in the flower labellum. Ref.1

Miscellaneous

Flowers 9-alkenes and 12-alkenes function as attractants to the pollinator Andrena nigroaenea by mimicry of the bee's sex pheromones (Ref.1).

Sequence similarities

Belongs to the fatty acid desaturase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3333Chloroplast Potential
Chain34 – 376343Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic
PRO_0000417064

Regions

Compositional bias21 – 244Poly-Pro

Sites

Metal binding1141Iron 1 By similarity
Metal binding1491Iron 1 By similarity
Metal binding1491Iron 2 By similarity
Metal binding1521Iron 1; via pros nitrogen By similarity
Metal binding2021Iron 2 By similarity
Metal binding2351Iron 1 By similarity
Metal binding2351Iron 2 By similarity
Metal binding2381Iron 2; via pros nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
E3PZS2 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: 1A262105E0B6CE52

FASTA37642,359
        10         20         30         40         50         60 
MELHLALRAS PLPAADPGRR PPPPRGNFAT NCTAAINSTH ISQEKFRSLD SWVEHNMLTF 

        70         80         90        100        110        120 
LKPVEKCWQP QDFLPDPSHL SAEELGDAVR EIHERAAEIP DEVWVCMVGN MVTEEALPTY 

       130        140        150        160        170        180 
QSLISSVLGG TVAGSTPWDR WIRGWSAEEN RHGDLLNKYL YLTGRLDMRQ VEKTIQYLIG 

       190        200        210        220        230        240 
SGMDVGVGNS ILCGFIYTCF QEKATFISHG NTARLAKHHG DTTLAKICGL VAADEKRHAV 

       250        260        270        280        290        300 
AYTNLMKKLF EVAPNESMLA FAHIMRAHVT MPASRMFDGR DPRLFTHFSA VTQKIGVYTV 

       310        320        330        340        350        360 
RDYGEMLDFF LKEWEISAVV DDLSPEGRQA QEYVCGLPEV MGKMAERADD RRKKLVNVGE 

       370 
PRYIPFSWIF NKQVCV

« Hide

References

[1]"Stearoyl-acyl carrier protein desaturases are associated with floral isolation in sexually deceptive orchids."
Schlueter P.M., Xu S., Gagliardini V., Whittle E., Shanklin J., Grossniklaus U., Schiestl F.P.
Proc. Natl. Acad. Sci. U.S.A. 108:5696-5701(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
Tissue: Flower.

Web resources

Protein Spotlight

Unusual liaisons - Issue 145 of December 2012

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FR688109 mRNA. Translation: CBW95566.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00199.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
[Graphical view]
PfamPF03405. FA_desaturase_2. 1 hit.
[Graphical view]
PIRSFPIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMSSF47240. Ferritin/RR_like. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSTAD2_OPHSP
AccessionPrimary (citable) accession number: E3PZS2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: January 11, 2011
Last modified: May 1, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

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