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Protein

Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic

Gene

SAD2

Organism
Ophrys sphegodes (Early spider orchid) (Arachnites aranifera)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons Delta9 and Delta(10) of the acyl chain. Converts palmitoyl-ACP to hexadecenoic acid by introduction of a cis double bond between carbons Delta4 and Delta5 of the acyl chain. Catalyzes saturated fatty acid 18:0 and 16:0 to generate 18:1 (delta-9) and 16:1 (delta-4) intermediates, expected to give rise to 9-alkenes and 12-alkenes, respectively.1 Publication

Catalytic activityi

Stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier-protein] + acceptor + 2 H2O.1 Publication

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi114 – 1141Iron 1By similarity
Metal bindingi149 – 1491Iron 1By similarity
Metal bindingi149 – 1491Iron 2By similarity
Metal bindingi152 – 1521Iron 1; via pros nitrogenBy similarity
Metal bindingi202 – 2021Iron 2By similarity
Metal bindingi235 – 2351Iron 1By similarity
Metal bindingi235 – 2351Iron 2By similarity
Metal bindingi238 – 2381Iron 2; via pros nitrogenBy similarity

GO - Molecular functioni

  1. acyl-[acyl-carrier-protein] desaturase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB
  2. unsaturated fatty acid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic (EC:1.14.19.2)
Alternative name(s):
Stearoyl-ACP desaturase 2
Gene namesi
Name:SAD2
OrganismiOphrys sphegodes (Early spider orchid) (Arachnites aranifera)
Taxonomic identifieri145953 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesOrchidaceaeOrchidoideaeOrchideaeOrchidinaeOphrys

Subcellular locationi

Plastidchloroplast stroma By similarity

GO - Cellular componenti

  1. chloroplast stroma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333ChloroplastSequence AnalysisAdd
BLAST
Chaini34 – 376343Acyl-[acyl-carrier-protein] desaturase 2, chloroplasticPRO_0000417064Add
BLAST

Expressioni

Tissue specificityi

Preferentially expressed in the flower labellum.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliE3PZS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi21 – 244Poly-Pro

Sequence similaritiesi

Belongs to the fatty acid desaturase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
[Graphical view]
PfamiPF03405. FA_desaturase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E3PZS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELHLALRAS PLPAADPGRR PPPPRGNFAT NCTAAINSTH ISQEKFRSLD
60 70 80 90 100
SWVEHNMLTF LKPVEKCWQP QDFLPDPSHL SAEELGDAVR EIHERAAEIP
110 120 130 140 150
DEVWVCMVGN MVTEEALPTY QSLISSVLGG TVAGSTPWDR WIRGWSAEEN
160 170 180 190 200
RHGDLLNKYL YLTGRLDMRQ VEKTIQYLIG SGMDVGVGNS ILCGFIYTCF
210 220 230 240 250
QEKATFISHG NTARLAKHHG DTTLAKICGL VAADEKRHAV AYTNLMKKLF
260 270 280 290 300
EVAPNESMLA FAHIMRAHVT MPASRMFDGR DPRLFTHFSA VTQKIGVYTV
310 320 330 340 350
RDYGEMLDFF LKEWEISAVV DDLSPEGRQA QEYVCGLPEV MGKMAERADD
360 370
RRKKLVNVGE PRYIPFSWIF NKQVCV
Length:376
Mass (Da):42,359
Last modified:January 11, 2011 - v1
Checksum:i1A262105E0B6CE52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR688109 mRNA. Translation: CBW95566.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Unusual liaisons - Issue 145 of December 2012

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR688109 mRNA. Translation: CBW95566.1.

3D structure databases

ProteinModelPortaliE3PZS2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00199.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
[Graphical view]
PfamiPF03405. FA_desaturase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Stearoyl-acyl carrier protein desaturases are associated with floral isolation in sexually deceptive orchids."
    Schlueter P.M., Xu S., Gagliardini V., Whittle E., Shanklin J., Grossniklaus U., Schiestl F.P.
    Proc. Natl. Acad. Sci. U.S.A. 108:5696-5701(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    Tissue: Flower.

Entry informationi

Entry nameiSTAD2_OPHSP
AccessioniPrimary (citable) accession number: E3PZS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: January 11, 2011
Last modified: January 7, 2015
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Flowers 9-alkenes and 12-alkenes function as attractants to the pollinator Andrena nigroaenea by mimicry of the bee's sex pheromones.1 Publication

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.