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E3PZS2

- STAD2_OPHSP

UniProt

E3PZS2 - STAD2_OPHSP

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Protein
Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic
Gene
SAD2
Organism
Ophrys sphegodes (Early spider orchid) (Arachnites aranifera)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons Delta9 and Delta(10) of the acyl chain. Converts palmitoyl-ACP to hexadecenoic acid by introduction of a cis double bond between carbons Delta4 and Delta5 of the acyl chain. Catalyzes saturated fatty acid 18:0 and 16:0 to generate 18:1 (delta-9) and 16:1 (delta-4) intermediates, expected to give rise to 9-alkenes and 12-alkenes, respectively.1 Publication

Catalytic activityi

Stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier-protein] + acceptor + 2 H2O.1 Publication

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi114 – 1141Iron 1 By similarity
Metal bindingi149 – 1491Iron 1 By similarity
Metal bindingi149 – 1491Iron 2 By similarity
Metal bindingi152 – 1521Iron 1; via pros nitrogen By similarity
Metal bindingi202 – 2021Iron 2 By similarity
Metal bindingi235 – 2351Iron 1 By similarity
Metal bindingi235 – 2351Iron 2 By similarity
Metal bindingi238 – 2381Iron 2; via pros nitrogen By similarity

GO - Molecular functioni

  1. acyl-[acyl-carrier-protein] desaturase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB
  2. unsaturated fatty acid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic (EC:1.14.19.2)
Alternative name(s):
Stearoyl-ACP desaturase 2
Gene namesi
Name:SAD2
OrganismiOphrys sphegodes (Early spider orchid) (Arachnites aranifera)
Taxonomic identifieri145953 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesOrchidaceaeOrchidoideaeOrchideaeOrchidinaeOphrys

Subcellular locationi

Plastidchloroplast stroma By similarity

GO - Cellular componenti

  1. chloroplast stroma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Chloroplast Reviewed prediction
Add
BLAST
Chaini34 – 376343Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic
PRO_0000417064Add
BLAST

Expressioni

Tissue specificityi

Preferentially expressed in the flower labellum.1 Publication

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliE3PZS2.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi21 – 244Poly-Pro

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
[Graphical view]
PfamiPF03405. FA_desaturase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E3PZS2-1 [UniParc]FASTAAdd to Basket

« Hide

MELHLALRAS PLPAADPGRR PPPPRGNFAT NCTAAINSTH ISQEKFRSLD    50
SWVEHNMLTF LKPVEKCWQP QDFLPDPSHL SAEELGDAVR EIHERAAEIP 100
DEVWVCMVGN MVTEEALPTY QSLISSVLGG TVAGSTPWDR WIRGWSAEEN 150
RHGDLLNKYL YLTGRLDMRQ VEKTIQYLIG SGMDVGVGNS ILCGFIYTCF 200
QEKATFISHG NTARLAKHHG DTTLAKICGL VAADEKRHAV AYTNLMKKLF 250
EVAPNESMLA FAHIMRAHVT MPASRMFDGR DPRLFTHFSA VTQKIGVYTV 300
RDYGEMLDFF LKEWEISAVV DDLSPEGRQA QEYVCGLPEV MGKMAERADD 350
RRKKLVNVGE PRYIPFSWIF NKQVCV 376
Length:376
Mass (Da):42,359
Last modified:January 11, 2011 - v1
Checksum:i1A262105E0B6CE52
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FR688109 mRNA. Translation: CBW95566.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Unusual liaisons - Issue 145 of December 2012

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FR688109 mRNA. Translation: CBW95566.1 .

3D structure databases

ProteinModelPortali E3PZS2.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00199 .

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
[Graphical view ]
Pfami PF03405. FA_desaturase_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMi SSF47240. SSF47240. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Stearoyl-acyl carrier protein desaturases are associated with floral isolation in sexually deceptive orchids."
    Schlueter P.M., Xu S., Gagliardini V., Whittle E., Shanklin J., Grossniklaus U., Schiestl F.P.
    Proc. Natl. Acad. Sci. U.S.A. 108:5696-5701(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    Tissue: Flower.

Entry informationi

Entry nameiSTAD2_OPHSP
AccessioniPrimary (citable) accession number: E3PZS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: January 11, 2011
Last modified: April 16, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Flowers 9-alkenes and 12-alkenes function as attractants to the pollinator Andrena nigroaenea by mimicry of the bee's sex pheromones (1 Publication).

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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