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E3PZS2

- STAD2_OPHSP

UniProt

E3PZS2 - STAD2_OPHSP

Protein

Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic

Gene

SAD2

Organism
Ophrys sphegodes (Early spider orchid) (Arachnites aranifera)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 17 (01 Oct 2014)
      Sequence version 1 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons Delta9 and Delta(10) of the acyl chain. Converts palmitoyl-ACP to hexadecenoic acid by introduction of a cis double bond between carbons Delta4 and Delta5 of the acyl chain. Catalyzes saturated fatty acid 18:0 and 16:0 to generate 18:1 (delta-9) and 16:1 (delta-4) intermediates, expected to give rise to 9-alkenes and 12-alkenes, respectively.1 Publication

    Catalytic activityi

    Stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier-protein] + acceptor + 2 H2O.1 Publication

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi114 – 1141Iron 1By similarity
    Metal bindingi149 – 1491Iron 1By similarity
    Metal bindingi149 – 1491Iron 2By similarity
    Metal bindingi152 – 1521Iron 1; via pros nitrogenBy similarity
    Metal bindingi202 – 2021Iron 2By similarity
    Metal bindingi235 – 2351Iron 1By similarity
    Metal bindingi235 – 2351Iron 2By similarity
    Metal bindingi238 – 2381Iron 2; via pros nitrogenBy similarity

    GO - Molecular functioni

    1. acyl-[acyl-carrier-protein] desaturase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB
    2. unsaturated fatty acid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    Iron, Metal-binding, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic (EC:1.14.19.2)
    Alternative name(s):
    Stearoyl-ACP desaturase 2
    Gene namesi
    Name:SAD2
    OrganismiOphrys sphegodes (Early spider orchid) (Arachnites aranifera)
    Taxonomic identifieri145953 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesOrchidaceaeOrchidoideaeOrchideaeOrchidinaeOphrys

    Subcellular locationi

    Plastidchloroplast stroma By similarity

    GO - Cellular componenti

    1. chloroplast stroma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333ChloroplastSequence AnalysisAdd
    BLAST
    Chaini34 – 376343Acyl-[acyl-carrier-protein] desaturase 2, chloroplasticPRO_0000417064Add
    BLAST

    Expressioni

    Tissue specificityi

    Preferentially expressed in the flower labellum.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliE3PZS2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi21 – 244Poly-Pro

    Sequence similaritiesi

    Belongs to the fatty acid desaturase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR005067. Fatty_acid_desaturase-2.
    IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    [Graphical view]
    PfamiPF03405. FA_desaturase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000346. Dlt9_acylACP_des. 1 hit.
    SUPFAMiSSF47240. SSF47240. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    E3PZS2-1 [UniParc]FASTAAdd to Basket

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    MELHLALRAS PLPAADPGRR PPPPRGNFAT NCTAAINSTH ISQEKFRSLD    50
    SWVEHNMLTF LKPVEKCWQP QDFLPDPSHL SAEELGDAVR EIHERAAEIP 100
    DEVWVCMVGN MVTEEALPTY QSLISSVLGG TVAGSTPWDR WIRGWSAEEN 150
    RHGDLLNKYL YLTGRLDMRQ VEKTIQYLIG SGMDVGVGNS ILCGFIYTCF 200
    QEKATFISHG NTARLAKHHG DTTLAKICGL VAADEKRHAV AYTNLMKKLF 250
    EVAPNESMLA FAHIMRAHVT MPASRMFDGR DPRLFTHFSA VTQKIGVYTV 300
    RDYGEMLDFF LKEWEISAVV DDLSPEGRQA QEYVCGLPEV MGKMAERADD 350
    RRKKLVNVGE PRYIPFSWIF NKQVCV 376
    Length:376
    Mass (Da):42,359
    Last modified:January 11, 2011 - v1
    Checksum:i1A262105E0B6CE52
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR688109 mRNA. Translation: CBW95566.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Unusual liaisons - Issue 145 of December 2012

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR688109 mRNA. Translation: CBW95566.1 .

    3D structure databases

    ProteinModelPortali E3PZS2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00199 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR005067. Fatty_acid_desaturase-2.
    IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    [Graphical view ]
    Pfami PF03405. FA_desaturase_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000346. Dlt9_acylACP_des. 1 hit.
    SUPFAMi SSF47240. SSF47240. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Stearoyl-acyl carrier protein desaturases are associated with floral isolation in sexually deceptive orchids."
      Schlueter P.M., Xu S., Gagliardini V., Whittle E., Shanklin J., Grossniklaus U., Schiestl F.P.
      Proc. Natl. Acad. Sci. U.S.A. 108:5696-5701(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
      Tissue: Flower.

    Entry informationi

    Entry nameiSTAD2_OPHSP
    AccessioniPrimary (citable) accession number: E3PZS2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2012
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 17 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Flowers 9-alkenes and 12-alkenes function as attractants to the pollinator Andrena nigroaenea by mimicry of the bee's sex pheromones.1 Publication

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3