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Protein

D-ornithine 4,5-aminomutase subunit alpha

Gene

oraS

Organism
Acetoanaerobium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / NCIMB 10654) (Clostridium sticklandii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a complex that catalyzes the reversible migration of the omega amino group of D-ornithine to C-4 to form (2R,4S)-2,4-diaminopentanoic acid. The role of OraS remains obscure; however, it seems to be required for a correct folding of the OraE subunit. The complex is active only on D-ornithine and 2,4-diaminopentanoic acid and not active on L-ornithine, L-beta-lysine, L-alpha-lysine or D-alpha-lysine.3 Publications

Catalytic activityi

D-ornithine = (2R,4S)-2,4-diaminopentanoate.3 Publications

Enzyme regulationi

Increased activity in the presence of dithiothreitol (DTT) in vitro. Inhibited by 1 mM potassium phosphate and potassium chloride. Inhibited by L-alpha-ornithine, D,L-alpha-lysine, L-beta-lysine (50%-60%), L-alpha-lysine (26%) and by delta-amino-n-valeric acid to a lesser extent. Significant decrease in activity is observed in the presence of 0.2 mM p-chloromercuribenzoate, N-ethylmaleimide and also by 2 mM iodoacetate to a lesser extent but not inhibited by arsenite.2 Publications

pH dependencei

Optimum pH is 9.0 or between 8.5-8.7 (with Tris-HCl buffer). Half-maximal activity is observed at pH 7.4 and 9.7.2 Publications

Temperature dependencei

Optimum temperature is 37 degrees Celsius for native enzyme. Displays half-maximal activity at 23 degrees Celsius and 49 degrees Celsius. Rapidly inactivated at temperatures above 45 degrees Celsius. Loses more than 35% and 30% of its activity when stored at -20 degrees Celsius for 1 month and at 4 degrees Celsius for 48 hours, respectively.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12492
BRENDAi5.4.3.5 1522

Names & Taxonomyi

Protein namesi
Recommended name:
D-ornithine 4,5-aminomutase subunit alpha (EC:5.4.3.5Imported)
Alternative name(s):
D-ornithine aminomutase S component1 Publication
Short name:
OAM-S
Gene namesi
Name:oraS
Ordered Locus Names:CLOST_1291
OrganismiAcetoanaerobium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / NCIMB 10654) (Clostridium sticklandii)
Taxonomic identifieri499177 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeAcetoanaerobium
Proteomesi
  • UP000007041 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004218041 – 121D-ornithine 4,5-aminomutase subunit alphaAdd BLAST121

Interactioni

Subunit structurei

Heterotetramer of 2 alpha (OraS) and 2 beta (OraE) subunits.3 Publications

Protein-protein interaction databases

STRINGi499177.CLOST_1291

Structurei

Secondary structure

1121
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 10Combined sources4
Helixi12 – 14Combined sources3
Helixi19 – 43Combined sources25
Helixi47 – 56Combined sources10
Helixi61 – 73Combined sources13
Helixi77 – 79Combined sources3
Helixi81 – 91Combined sources11
Helixi96 – 104Combined sources9
Helixi109 – 113Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KOWX-ray2.90E/F/G/H1-121[»]
3KOXX-ray2.40E/F/G/H1-121[»]
3KOYX-ray2.80E/F/G/H1-121[»]
3KOZX-ray2.80E/F/G/H1-121[»]
3KP0X-ray2.80E/F/G/H1-121[»]
3KP1X-ray2.01E/F/G/H1-121[»]
SMRiE3PY96
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiE3PY96

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105KIJ Bacteria
ENOG4111S56 LUCA
HOGENOMiHOG000292272
KOiK17899
OMAiLGKGAGH
OrthoDBiPOG091H0UGX

Family and domain databases

InterProiView protein in InterPro
IPR016176 Cbl-dep_enz_cat
IPR015130 Lys-AminoMut_A
PfamiView protein in Pfam
PF16552 OAM_alpha, 1 hit
SUPFAMiSSF51703 SSF51703, 1 hit

Sequencei

Sequence statusi: Complete.

E3PY96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRADDFQQR RAHLANLSDE ELQTRFWEMA EKIVDPLLDL GKKNTTPSIE
60 70 80 90 100
RSVLLRMGFS SLEAKAIVDK TMDRGLMGKG AGHIVYKIAK EKNISVREAG
110 120
LALSEGKYWD DAIQIFKGGV K
Length:121
Mass (Da):13,623
Last modified:March 8, 2011 - v1
Checksum:i62D4FC5EA0087F86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY038595 Genomic DNA Translation: AAK72501.1
FP565809 Genomic DNA Translation: CBH21411.1
RefSeqiWP_013361504.1, NC_014614.1

Genome annotation databases

EnsemblBacteriaiCBH21411; CBH21411; CLOST_1291
GeneIDi35559290
KEGGicst:CLOST_1291

Similar proteinsi

Entry informationi

Entry nameiOAMS_ACESD
AccessioniPrimary (citable) accession number: E3PY96
Secondary accession number(s): Q8VPJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: March 8, 2011
Last modified: March 28, 2018
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health