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Protein
Submitted name:

Protein chain elongation factor EF-Tu (Duplicate of tufA)

Gene

tufB

Organism
Clostridium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / NCIB 10654)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi82 – 865GTPUniRule annotation
Nucleotide bindingi137 – 1404GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factorUniRule annotationImported

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSTI499177:GJE9-2272-MONOMER.
CSTI499177:GJE9-2286-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
Protein chain elongation factor EF-Tu (Duplicate of tufA)Imported
Gene namesi
Name:tufBImported
Synonyms:tufUniRule annotation
Ordered Locus Names:CLOST_2206Imported, CLOST_2219Imported
OrganismiClostridium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / NCIB 10654)Imported
Taxonomic identifieri499177 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium
ProteomesiUP000007041 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229290.
KOiK02358.
OMAiDEGGRHN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E3PTX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKAKFERTK PHVNIGTIGH VDHGKTTLTA AITKTLHARY GFGAAVDFEN
60 70 80 90 100
IDKAPEERER GITISTAHVE YETPNRHYAH VDCPGHADYV KNMITGAAQM
110 120 130 140 150
DGAILVCSAA DGPMPQTREH ILLSRQVGVP YIVVFLNKCD MVDDEELLEL
160 170 180 190 200
VEMEVRDLLN EYEFPGDDTP IIRGSALKAL EDPASEWGDK IVELFEQIDS
210 220 230 240 250
YIPEPERDID KPFLMPVEDV FSITGRGTVA TGRVERGILK VQDEVEIVGL
260 270 280 290 300
TEEARKVVVT GVEMFRKLLD QAQAGDNIGA LLRGVTRDDI ERGQVLAKSG
310 320 330 340 350
TIKPRTKFTA EIYVLKKEEG GRHTPFFKGY RPQFYFRTTD VTGDITLPDG
360 370 380 390
VEMVMPGDNI TISVELITPI AMEEGLRFAI REGGRTVGAG VVATIVE
Length:397
Mass (Da):43,882
Last modified:January 10, 2011 - v1
Checksum:i77425217445FA464
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP565809 Genomic DNA. Translation: CBH22326.1.
FP565809 Genomic DNA. Translation: CBH22339.1.
RefSeqiYP_003937231.1. NC_014614.1.
YP_003937244.1. NC_014614.1.

Genome annotation databases

EnsemblBacteriaiCBH22326; CBH22326; CLOST_2206.
CBH22339; CBH22339; CLOST_2219.
KEGGicst:CLOST_2206.
cst:CLOST_2219.
PATRICi42275041. VBICloSti32817_2192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP565809 Genomic DNA. Translation: CBH22326.1.
FP565809 Genomic DNA. Translation: CBH22339.1.
RefSeqiYP_003937231.1. NC_014614.1.
YP_003937244.1. NC_014614.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBH22326; CBH22326; CLOST_2206.
CBH22339; CBH22339; CLOST_2219.
KEGGicst:CLOST_2206.
cst:CLOST_2219.
PATRICi42275041. VBICloSti32817_2192.

Phylogenomic databases

HOGENOMiHOG000229290.
KOiK02358.
OMAiDEGGRHN.

Enzyme and pathway databases

BioCyciCSTI499177:GJE9-2272-MONOMER.
CSTI499177:GJE9-2286-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Clostridium sticklandii, a specialist in amino acid degradation:revisiting its metabolism through its genome sequence."
    Fonknechten N., Chaussonnerie S., Tricot S., Lajus A., Andreesen J.R., Perchat N., Pelletier E., Gouyvenoux M., Barbe V., Salanoubat M., Le Paslier D., Weissenbach J., Cohen G.N., Kreimeyer A.
    BMC Genomics 11:555-555(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 12662 / DSM 519 / JCM 1433 / NCIB 10654Imported.
  2. Genoscope - CEA
    Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 519Imported.

Entry informationi

Entry nameiE3PTX2_CLOSD
AccessioniPrimary (citable) accession number: E3PTX2
Entry historyi
Integrated into UniProtKB/TrEMBL: January 10, 2011
Last sequence update: January 10, 2011
Last modified: March 31, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.