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Protein
Submitted name:

Sucrose:(Sucrose/fructan) 6-fructosyltransferase

Gene

6-SFT

Organism
Pachysandra terminalis
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase, TransferaseImported

Enzyme and pathway databases

BRENDAi3.2.1.154. 8994.

Names & Taxonomyi

Protein namesi
Submitted name:
Sucrose:(Sucrose/fructan) 6-fructosyltransferaseImported
Gene namesi
Name:6-SFTImported
Synonyms:6-SSTImported
OrganismiPachysandra terminalisImported
Taxonomic identifieri74825 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsBuxalesBuxaceaePachysandra

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei45 – 6319HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi168 – 1681N-linked (GlcNAc...)Combined sourcesCAR_5005389561
Glycosylationi287 – 2871N-linked (GlcNAc...)Combined sourcesCAR_5005389562
Glycosylationi509 – 5091N-linked (GlcNAc...)Combined sourcesCAR_5005389563
Disulfide bondi510 ↔ 558Combined sources
Glycosylationi630 – 6301N-linked (GlcNAc...)Combined sourcesCAR_5005389564

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UGFX-ray1.70A/B110-655[»]
3UGGX-ray2.90A/B110-655[»]
3UGHX-ray2.90A/B110-655[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 124103DUF3357InterPro annotationAdd
BLAST
Domaini132 – 450319Glyco_hydro_32NInterPro annotationAdd
BLAST
Domaini523 – 60583Glyco_hydro_32CInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 32 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
2.60.120.560. 2 hits.
InterProiIPR021792. Beta-fructofuranosidase.
IPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PfamiPF11837. DUF3357. 1 hit.
PF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTiSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF75005. SSF75005. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E3PQS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDINPFLAF PDQEGDVAVP CSYTSLPDEA QSEEIPASRS KLMKGFLIFF
60 70 80 90 100
SGILMLGLLM VFVSDQGSKT PSSLSSSVMA LVSTSTSPAR INPISRGVPE
110 120 130 140 150
GVPEKAFHMF PDNAVPYPWS NAQLSWQRTA FHFQPERSWM SDPDGPIFYK
160 170 180 190 200
GWYHFFYQYN PDNPVWGNNT WGHTVSRDLI HWLYLPLALA ADQWYDMQGV
210 220 230 240 250
FSGSATCLPD GRIMMLYTGV TKEMVEMLSL AYPADLSDPL LVEWVKYPGN
260 270 280 290 300
PILSAPPGVS PTEFRDASTG WYVSNGTWRI AIGAKYNTTG IAMVYETKDF
310 320 330 340 350
KSFKLLEELL HAVPDTGLWE CVDLYPVSTT GEKGLETSVN GPKVKHVLKA
360 370 380 390 400
SIDEQQRDYY AIGTYDLGTN KWTPDNPEED VGIGLRYDWG KYYASKTFYD
410 420 430 440 450
PKKQRRVVWA WTKELDSEVA DREKGWANVQ TIPRTVLLDQ KTGTNVLLWP
460 470 480 490 500
VEEVESLRLS SKEFSKVKAG AGSVVPLDVG TATQLDIIAE FEIDKEALEG
510 520 530 540 550
TIEADMGYNC TTSGGAAERG VLGPFGLLVS ATENLSEQTP VYFYIAKGTD
560 570 580 590 600
GNFKTFFCLD ESRSSKASDV SKQVKGFTVP VLDGEKFTMR LLVDHSIVES
610 620 630 640 650
FAQGGRSCIT SRVYPTEAIY GAAKLFLFNN ATGASITASL KIWEMNSAFI

QPFHR
Length:655
Mass (Da):72,778
Last modified:March 21, 2012 - v2
Checksum:i27130ECB2FD6A44B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN870376 mRNA. Translation: CBM41476.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN870376 mRNA. Translation: CBM41476.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UGFX-ray1.70A/B110-655[»]
3UGGX-ray2.90A/B110-655[»]
3UGHX-ray2.90A/B110-655[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.1.154. 8994.

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
2.60.120.560. 2 hits.
InterProiIPR021792. Beta-fructofuranosidase.
IPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PfamiPF11837. DUF3357. 1 hit.
PF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTiSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF75005. SSF75005. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Unexpected presence of graminan- and levan-type fructans in the evergreen frost-hardy eudicot Pachysandra terminalis (Buxaceae): purification, cloning, and functional analysis of a 6-SST/6-SFT enzyme."
    Van den Ende W., Coopman M., Clerens S., Vergauwen R., Le Roy K., Lammens W., Van Laere A.
    Plant Physiol. 155:603-614(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: RhizomeImported.
  2. "Crystal structure of 6-SST/6-SFT from Pachysandra terminalis, a plant fructan biosynthesizing enzyme in complex with its acceptor substrate 6-kestose."
    Lammens W., Le Roy K., Yuan S., Vergauwen R., Rabijns A., Van Laere A., Strelkov S.V., Van den Ende W.
    Plant J. 70:205-219(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 110-655, DISULFIDE BONDS, GLYCOSYLATION AT ASN-168; ASN-287; ASN-509 AND ASN-630.
  3. Wikstroem N.
    Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: RhizomeImported.

Entry informationi

Entry nameiE3PQS3_9MAGN
AccessioniPrimary (citable) accession number: E3PQS3
Entry historyi
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: March 21, 2012
Last modified: June 8, 2016
This is version 30 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.