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Protein

DNA topoisomerase

Gene

Cre-top-3

Organism
Caenorhabditis remanei (Caenorhabditis vulgaris)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, Isomerase, TopoisomeraseUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomeraseUniRule annotation (EC:5.99.1.2UniRule annotation)
Gene namesi
Name:Cre-top-3Imported
ORF Names:CRE_00981Imported
OrganismiCaenorhabditis remanei (Caenorhabditis vulgaris)Imported
Taxonomic identifieri31234 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000008281 Componenti: Unassembled WGS sequence

Interactioni

Protein-protein interaction databases

STRINGi31234.CRE00981

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 147ToprimInterPro annotationAdd BLAST145

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1956 Eukaryota
COG0550 LUCA
InParanoidiE3MI31
OMAiFKAIQAF
OrthoDBiEOG091G03BD

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
IPR010666 Znf_GRF
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF06839 zf-GRF, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

E3MI31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKALFVAEK NDVAKGVASI LSNGTSNRKE GRSIFNKIYT LNTELFGQRT
60 70 80 90 100
AVSVTSVSGH MMNFQFHENM SNWQTTSMVE LFEAPVRHVV MPDMKLIEQT
110 120 130 140 150
LREEAQRHDL LVVWTDCDRE GEAIGAEIVK VCRESNRRID IFRARFSEIT
160 170 180 190 200
KTAITRAAQN LIRLDERTVA AVDCRSELDL RIGSAFTRLQ TLHLRNRFRD
210 220 230 240 250
LLGQNENSKV ISYGSCQFPT LGFVTDRYKL IENFISEPFW KLSVEHSREG
260 270 280 290 300
HKVEFLWDRN RLFDREVVDI LHDECKEAKE AHVEKVTKKP KSKWRPQGLD
310 320 330 340 350
TVELEKLGIS KLRMSAKQTM QVAEKLYSKG FISYPRTETN KFPAGLNLTP
360 370 380 390 400
LVEQQTHSNI WGDFATEVFI NFLINYYPSP FSAHPPIHPL TFADKSQLHG
410 420 430 440 450
DDWKVYELVV RHFLACVSQD AQGEETMVNL SIGSEKFHSS GLRIRDMGYL
460 470 480 490 500
KVYIYEKWGN RLLPTYTQDE RFSDYQLKIG DGKTQPPDLL TEADLIALMD
510 520 530 540 550
KFGIGTDATH AEHIEKIKSR EYIGVKPDGK LIPSFLGLAL VDGYDDMGFA
560 570 580 590 600
MSKPDLRANL EIQLNEICNG RRQKQDVLNE QIGKYKTIFV ESERNIEVLS
610 620 630 640 650
QSLQRYLNKN QQIGGGGGGN GGGGGPPRGP GGGGGGGGSK GPPKPPPKPR
660 670 680 690 700
GRPPNKSVTP TKDDDQENDT VRILLYLLPS SLHSIVHKSI ATLSEVLSNL
710 720 730 740 750
KNPKPARKSR AKKSVPKADA SKKGQENEFE EEEEVMCTCA EPMKAIMKVV
760 770 780
QKEGPNKGKK FYTCSLPYTS SDKCNFFKWV
Length:780
Mass (Da):88,072
Last modified:January 11, 2011 - v1
Checksum:i6E2D72E2ED82EB11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS268447 Genomic DNA Translation: EFP02335.1
RefSeqiXP_003104074.1, XM_003104026.1

Genome annotation databases

EnsemblMetazoaiCRE00981; CRE00981; WBGene00062997
GeneIDi9802799

Similar proteinsi

Entry informationi

Entry nameiE3MI31_CAERE
AccessioniPrimary (citable) accession number: E3MI31
Entry historyiIntegrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: March 28, 2018
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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