Pheromone-processing carboxypeptidase KEX1 precursor - Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)

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E3L8A5 (KEX1_PUCGT) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 16. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pheromone-processing carboxypeptidase KEX1
EC=3.4.16.6

Alternative name(s):
Carboxypeptidase D

Gene names

Name:	KEX1
ORF Names:	PGTG_18559

Organism

Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Pucciniomycotina › Pucciniomycetes › Pucciniales › Pucciniaceae › Puccinia ›

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death By similarity.
Catalytic activity	Preferential release of a C-terminal arginine or lysine residue.
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S10 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Carboxypeptidase Hydrolase Protease
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	serine-type carboxypeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – ?

Potential

Chain

? – 656

Pheromone-processing carboxypeptidase KEX1

PRO_0000411942

Regions

Topological domain

? – 559

Lumenal Potential

Transmembrane

21

Helical; Potential

Topological domain

76

Cytoplasmic Potential

Compositional bias

6

Poly-Ser

Compositional bias

4

Poly-Thr

Compositional bias

4

Poly-Lys

Compositional bias

5

Poly-His

Sites

Active site

1

By similarity

Active site

1

By similarity

Active site

1

By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

E3L8A5 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: 0FD95B1854B5F6CC
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656

72,697

        10         20         30         40         50         60 
MSSFQSATTR QGLHRRNMNS ETPQSEFIQR LRRSPPSESS SSTPSSSTTT SSTTTTSNTS 

        70         80         90        100        110        120 
GKKKKTAPSA SDFYVPSLPG QPKDSQLILY AGHLSFSPPD TTIEPEKDSY GFFFLNKARH 

       130        140        150        160        170        180 
IANRPVLLVW LNGGPGCSSF DGSLMEVGPL RMVLKGDGTL KEVDAAWNEY ANMLFIDQPT 

       190        200        210        220        230        240 
GTGYSYGPKP NYVHELDVSS ANLVNLLARF FKIFPEYQQM DLYICGESFA GQYIPYLAQA 

       250        260        270        280        290        300 
ILDTNIISAP LKGIMIGNGW IDPINQYLAY PEFAFKVGLV NPSSKAADLV NEELKKCTEW 

       310        320        330        340        350        360 
IDSNSTTPIH IEACEGILSA ITDSTVQTVN SQKMCLNMYD VRLVDSYPAC GLTWPPDLAD 

       370        380        390        400        410        420 
ITPYLSRTDV KQALHAQDHA ADWVECEAKV GNNFWAKTSQ PSVTLFPKLL DKIKILLFSG 

       430        440        450        460        470        480 
DQDLICCHTG TERMIDHLTW AGHQGWTSQA INQPWKVNGS YAGLWKEERN LTYVLVANAS 

       490        500        510        520        530        540 
HMAPYDVPYV TQDMLVRFLG IDVMTAAGPA AQITSRIGEE TATKVNRVVM NETKLGQEGS 

       550        560        570        580        590        600 
GTTGLAMTSG PVHDENYYNA SSAMLFLTLV GLVVGLIFFV RRRLRRDGHG DFAHHPSDET 

       610        620        630        640        650 
EIILKNHHHH HLSPDSSLPR SARDRQFQPV PTDDLDDHHL HPNLPSNNTS YQDLPR

References

[1]

"Obligate biotrophy features unraveled by the genomic analysis of rust fungi."
Duplessis S., Cuomo C.A., Lin Y.-C., Aerts A., Tisserant E., Veneault-Fourrey C., Joly D.L., Hacquard S., Amselem J., Cantarel B.L., Chiu R., Coutinho P.M., Feau N., Field M., Frey P., Gelhaye E., Goldberg J., Grabherr M.G.

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Martin F.
Proc. Natl. Acad. Sci. U.S.A. 108:9166-9171(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CRL 75-36-700-3 / race SCCL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS178373 Genomic DNA. Translation: EFP92780.2.
RefSeq	XP_003337199.2. XM_003337151.2.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S10.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10542492.
KEGG	pgr:PGTG_18559.
Family and domain databases
InterPro	IPR001563. Peptidase_S10. IPR018202. Peptidase_S10_AS. [Graphical view]
PANTHER	PTHR11802. PTHR11802. 1 hit.
Pfam	PF00450. Peptidase_S10. 1 hit. [Graphical view]
PRINTS	PR00724. CRBOXYPTASEC.
PROSITE	PS00560. CARBOXYPEPT_SER_HIS. 1 hit. PS00131. CARBOXYPEPT_SER_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KEX1_PUCGT

Accession

Primary (citable) accession number: E3L8A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	October 3, 2012
Last modified:	March 6, 2013
This is version 16 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents