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E3L3Q8

- MAP2_PUCGT

UniProt

E3L3Q8 - MAP2_PUCGT

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Protein

Methionine aminopeptidase 2

Gene

PGTG_16374

Organism
Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei209 – 2091SubstrateUniRule annotation
Metal bindingi229 – 2291Divalent metal cation 1UniRule annotation
Metal bindingi240 – 2401Divalent metal cation 1UniRule annotation
Metal bindingi240 – 2401Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi309 – 3091Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
Binding sitei317 – 3171SubstrateUniRule annotation
Metal bindingi343 – 3431Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi437 – 4371Divalent metal cation 1UniRule annotation
Metal bindingi437 – 4371Divalent metal cation 2; catalyticUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal involved in protein maturation Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2UniRule annotation
Short name:
MetAP 2UniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
ORF Names:PGTG_16374
OrganismiPuccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
Taxonomic identifieri418459 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaPucciniomycotinaPucciniomycetesPuccinialesPucciniaceaePuccinia
ProteomesiUP000008783: Unassembled WGS sequence

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Methionine aminopeptidase 2PRO_0000407666Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliE3L3Q8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi66 – 7914Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

InParanoidiE3L3Q8.
KOiK01265.
OMAiIQICEEL.
OrthoDBiEOG7BGHW3.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.

Sequencei

Sequence statusi: Complete.

E3L3Q8 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTIPVPKPAH PAAPDTNTDG LPNPKVVVPV EADEEEDDDD EEGKEDADGA
60 70 80 90 100
VPAASHFSTH ALDGTKKKKK KKKKPKKKKT GEAGAGGQSE PPRVPVSKLF
110 120 130 140 150
PNGNYPSGEE SAYLGENSYR TTSSEKRELE RLAAQEDPES AENYNSIRRA
160 170 180 190 200
AEVHRQVRRY VQQTVKPGMS MTEIAEMVED GTRALVEVDG LQRGIGFPTG
210 220 230 240 250
VSLNHCAAHY TPNAGDTIVL SADDVLKVDF GVQIGGRIVD SAFTMTFNNK
260 270 280 290 300
YDKLLEAVRA ATNTGIREAG IDARLSDIGA SIQETMESYE VEVDGKVHKV
310 320 330 340 350
KSIRNLTGHN ILPYHIHGGK SVPIVANSDE SAIMEEGDHF AVETFGSTGR
360 370 380 390 400
GYVMDDGECS HYAKNPDVNK PIRLARAKTL LNTINKHFDT LPFCKRYLDR
410 420 430 440 450
LGESRYYAAL DNLVNLGIVQ AYPPLSDIQG CMTAQYEHTI ILRPTCKEVV

SRGDDY
Length:456
Mass (Da):49,884
Last modified:January 11, 2011 - v1
Checksum:i3E186ACE45D67CED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS178343 Genomic DNA. Translation: EFP91183.1.
RefSeqiXP_003335602.1. XM_003335554.1.

Genome annotation databases

EnsemblFungiiEFP91183; EFP91183; PGTG_16374.
GeneIDi10528845.
KEGGipgr:PGTG_16374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS178343 Genomic DNA. Translation: EFP91183.1 .
RefSeqi XP_003335602.1. XM_003335554.1.

3D structure databases

ProteinModelPortali E3L3Q8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFP91183 ; EFP91183 ; PGTG_16374 .
GeneIDi 10528845.
KEGGi pgr:PGTG_16374.

Phylogenomic databases

InParanoidi E3L3Q8.
KOi K01265.
OMAi IQICEEL.
OrthoDBi EOG7BGHW3.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPi MF_03175. MetAP_2_euk.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR10804:SF9. PTHR10804:SF9. 1 hit.
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 2 hits.
TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CRL 75-36-700-3 / race SCCL.

Entry informationi

Entry nameiMAP2_PUCGT
AccessioniPrimary (citable) accession number: E3L3Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: January 11, 2011
Last modified: October 29, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3