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Protein

S-methyl-5'-thioadenosine phosphorylase 1

Gene

PGTG_06409

Organism
Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141PhosphateUniRule annotation
Sitei180 – 1801Important for substrate specificityUniRule annotation
Binding sitei198 – 1981Substrate; via amide nitrogenUniRule annotation
Binding sitei199 – 1991PhosphateUniRule annotation
Sitei235 – 2351Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. mRNA binding Source: EnsemblFungi
  2. phosphorylase activity Source: InterPro
  3. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamate biosynthetic process Source: EnsemblFungi
  2. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  3. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylase 1UniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylase 1UniRule annotation
Short name:
MTA phosphorylase 1UniRule annotation
Short name:
MTAP 1UniRule annotation
Short name:
MTAPase 1UniRule annotation
Gene namesi
ORF Names:PGTG_06409
OrganismiPuccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
Taxonomic identifieri418459 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaPucciniomycotinaPucciniomycetesPuccinialesPucciniaceaePuccinia
ProteomesiUP000008783: Unassembled WGS sequence

Subcellular locationi

Cytoplasm UniRule annotation. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303S-methyl-5'-thioadenosine phosphorylase 1PRO_0000415133Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 582Phosphate bindingUniRule annotation
Regioni90 – 912Phosphate bindingUniRule annotation
Regioni222 – 2243Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

InParanoidiE3K7C3.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiEOG77DJGM.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E3K7C3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGHAPLVGV IGGSGLYKLE GIEPVESLNI DTPWGRPSSP ITLFKLPSGP
60 70 80 90 100
VVAFLARHGV SHQFTPSEVP SRANIAALKK IGCQVIIAFS AVGSLREEIK
110 120 130 140 150
PRDIVVPSQI IDRTKSVRPC TFFEGLGVVG HAMFGEPFDT ELTGLVTKSI
160 170 180 190 200
KEAVTGFEMN DRIGVHAEKV AICMEGPAFS TRAESNMYRM FGGDIINMSV
210 220 230 240 250
LPEAKLAREA ELSYALIAQI TDYDAWRESE EPVTVAEVMA TIAANVSVSN
260 270 280 290 300
RLTLTILDEV HNAVAKGQLK TCKGTMEYSV MTKKEMISEE SKKTLSFILP

YFS
Length:303
Mass (Da):32,896
Last modified:January 11, 2011 - v1
Checksum:iADA699B2E9CF9AE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS178275 Genomic DNA. Translation: EFP80453.1.
RefSeqiXP_003324872.1. XM_003324824.2.

Genome annotation databases

EnsemblFungiiEFP80453; EFP80453; PGTG_06409.
GeneIDi10539462.
KEGGipgr:PGTG_06409.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS178275 Genomic DNA. Translation: EFP80453.1.
RefSeqiXP_003324872.1. XM_003324824.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFP80453; EFP80453; PGTG_06409.
GeneIDi10539462.
KEGGipgr:PGTG_06409.

Phylogenomic databases

InParanoidiE3K7C3.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiEOG77DJGM.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CRL 75-36-700-3 / race SCCL.

Entry informationi

Entry nameiMTAP1_PUCGT
AccessioniPrimary (citable) accession number: E3K7C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 11, 2011
Last modified: January 7, 2015
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.