Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-methyl-5'-thioadenosine phosphorylase 1

Gene

PGTG_06409

Organism
Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase 2 (PGTG_06407), S-methyl-5'-thioadenosine phosphorylase 1 (PGTG_06409)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14PhosphateUniRule annotation1
Sitei180Important for substrate specificityUniRule annotation1
Binding sitei198Substrate; via amide nitrogenUniRule annotation1
Binding sitei199PhosphateUniRule annotation1
Sitei235Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylase 1UniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylase 1UniRule annotation
Short name:
MTA phosphorylase 1UniRule annotation
Short name:
MTAP 1UniRule annotation
Short name:
MTAPase 1UniRule annotation
Gene namesi
ORF Names:PGTG_06409
OrganismiPuccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
Taxonomic identifieri418459 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaPucciniomycotinaPucciniomycetesPuccinialesPucciniaceaePuccinia
Proteomesi
  • UP000008783 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:PGTG_06409.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004151331 – 303S-methyl-5'-thioadenosine phosphorylase 1Add BLAST303

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 58Phosphate bindingUniRule annotation2
Regioni90 – 91Phosphate bindingUniRule annotation2
Regioni222 – 224Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

InParanoidiE3K7C3.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiEOG092C46SL.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP. 1 hit.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E3K7C3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGHAPLVGV IGGSGLYKLE GIEPVESLNI DTPWGRPSSP ITLFKLPSGP
60 70 80 90 100
VVAFLARHGV SHQFTPSEVP SRANIAALKK IGCQVIIAFS AVGSLREEIK
110 120 130 140 150
PRDIVVPSQI IDRTKSVRPC TFFEGLGVVG HAMFGEPFDT ELTGLVTKSI
160 170 180 190 200
KEAVTGFEMN DRIGVHAEKV AICMEGPAFS TRAESNMYRM FGGDIINMSV
210 220 230 240 250
LPEAKLAREA ELSYALIAQI TDYDAWRESE EPVTVAEVMA TIAANVSVSN
260 270 280 290 300
RLTLTILDEV HNAVAKGQLK TCKGTMEYSV MTKKEMISEE SKKTLSFILP

YFS
Length:303
Mass (Da):32,896
Last modified:January 11, 2011 - v1
Checksum:iADA699B2E9CF9AE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS178275 Genomic DNA. Translation: EFP80453.1.
RefSeqiXP_003324872.1. XM_003324824.2.

Genome annotation databases

EnsemblFungiiEFP80453; EFP80453; PGTG_06409.
GeneIDi10539462.
KEGGipgr:PGTG_06409.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS178275 Genomic DNA. Translation: EFP80453.1.
RefSeqiXP_003324872.1. XM_003324824.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFP80453; EFP80453; PGTG_06409.
GeneIDi10539462.
KEGGipgr:PGTG_06409.

Organism-specific databases

EuPathDBiFungiDB:PGTG_06409.

Phylogenomic databases

InParanoidiE3K7C3.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiEOG092C46SL.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP. 1 hit.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTAP1_PUCGT
AccessioniPrimary (citable) accession number: E3K7C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 11, 2011
Last modified: September 7, 2016
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.