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Protein

S-methyl-5'-thioadenosine phosphorylase 2

Gene

PGTG_06407

Organism
Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathway: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase 2 (PGTG_06407), S-methyl-5'-thioadenosine phosphorylase 1 (PGTG_06409)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141PhosphateUniRule annotation
Sitei167 – 1671Important for substrate specificityUniRule annotation
Binding sitei185 – 1851Substrate; via amide nitrogenUniRule annotation
Binding sitei186 – 1861PhosphateUniRule annotation
Sitei222 – 2221Important for substrate specificityUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylase 2UniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylase 2UniRule annotation
Short name:
MTA phosphorylase 2UniRule annotation
Short name:
MTAP 2UniRule annotation
Short name:
MTAPase 2UniRule annotation
Gene namesi
ORF Names:PGTG_06407
OrganismiPuccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
Taxonomic identifieri418459 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaPucciniomycotinaPucciniomycetesPuccinialesPucciniaceaePuccinia
ProteomesiUP000008783 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:PGTG_06407.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290S-methyl-5'-thioadenosine phosphorylase 2PRO_0000415134Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi5297.EFP80451.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 582Phosphate bindingUniRule annotation
Regioni90 – 912Phosphate bindingUniRule annotation
Regioni209 – 2113Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

InParanoidiE3K7C1.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG77DJGM.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E3K7C1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGHAPLVGV IGGSGLYKLE GIEPVESLNI DTPWGRPSSP ITLFKLPSGP
60 70 80 90 100
VVAFLARHGV SHQFTPSEVP SRANIAALKK IGCQVIIAFS AVGSLREEIK
110 120 130 140 150
PRDIVVPSQI IDRTKSAHAM FGEPFDTELT GLVTKSIKEA VTGFEMNDRI
160 170 180 190 200
GVHAEKVAIC MEGPAFSTRA ESNMYRMFGG DIINMSVLPE AKLAREAELS
210 220 230 240 250
YALIAQITDY DAWRESEEPV TVAEVMATIA ANVSVSNRLT LTILDEVHNA
260 270 280 290
VAKGQLKTCK GTMEYSVMTK KEMISEESKK TLSFILPYFS
Length:290
Mass (Da):31,504
Last modified:January 11, 2011 - v1
Checksum:i5266EC7645DE8A51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS178275 Genomic DNA. Translation: EFP80451.1.
RefSeqiXP_003324870.1. XM_003324822.1.

Genome annotation databases

EnsemblFungiiEFP80451; EFP80451; PGTG_06407.
GeneIDi10539460.
KEGGipgr:PGTG_06407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS178275 Genomic DNA. Translation: EFP80451.1.
RefSeqiXP_003324870.1. XM_003324822.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5297.EFP80451.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFP80451; EFP80451; PGTG_06407.
GeneIDi10539460.
KEGGipgr:PGTG_06407.

Organism-specific databases

EuPathDBiFungiDB:PGTG_06407.

Phylogenomic databases

InParanoidiE3K7C1.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG77DJGM.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CRL 75-36-700-3 / race SCCL.

Entry informationi

Entry nameiMTAP2_PUCGT
AccessioniPrimary (citable) accession number: E3K7C1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 11, 2011
Last modified: June 24, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.