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E3K7C1

- MTAP2_PUCGT

UniProt

E3K7C1 - MTAP2_PUCGT

Protein

S-methyl-5'-thioadenosine phosphorylase 2

Gene

PGTG_06407

Organism
Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

    Catalytic activityi

    S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141PhosphateUniRule annotation
    Sitei167 – 1671Important for substrate specificityUniRule annotation
    Binding sitei185 – 1851Substrate; via amide nitrogenUniRule annotation
    Binding sitei186 – 1861PhosphateUniRule annotation
    Sitei222 – 2221Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    UniPathwayiUPA00904; UER00873.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioadenosine phosphorylase 2UniRule annotation (EC:2.4.2.28UniRule annotation)
    Alternative name(s):
    5'-methylthioadenosine phosphorylase 2UniRule annotation
    Short name:
    MTA phosphorylase 2UniRule annotation
    Short name:
    MTAP 2UniRule annotation
    Short name:
    MTAPase 2UniRule annotation
    Gene namesi
    ORF Names:PGTG_06407
    OrganismiPuccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
    Taxonomic identifieri418459 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaPucciniomycotinaPucciniomycetesPuccinialesPucciniaceaePuccinia
    ProteomesiUP000008783: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm UniRule annotation. Nucleus UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 290290S-methyl-5'-thioadenosine phosphorylase 2PRO_0000415134Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni57 – 582Phosphate bindingUniRule annotation
    Regioni90 – 912Phosphate bindingUniRule annotation
    Regioni209 – 2113Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    KOiK00772.
    OMAiCEAQLCY.
    OrthoDBiEOG77DJGM.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E3K7C1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGHAPLVGV IGGSGLYKLE GIEPVESLNI DTPWGRPSSP ITLFKLPSGP    50
    VVAFLARHGV SHQFTPSEVP SRANIAALKK IGCQVIIAFS AVGSLREEIK 100
    PRDIVVPSQI IDRTKSAHAM FGEPFDTELT GLVTKSIKEA VTGFEMNDRI 150
    GVHAEKVAIC MEGPAFSTRA ESNMYRMFGG DIINMSVLPE AKLAREAELS 200
    YALIAQITDY DAWRESEEPV TVAEVMATIA ANVSVSNRLT LTILDEVHNA 250
    VAKGQLKTCK GTMEYSVMTK KEMISEESKK TLSFILPYFS 290
    Length:290
    Mass (Da):31,504
    Last modified:January 11, 2011 - v1
    Checksum:i5266EC7645DE8A51
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS178275 Genomic DNA. Translation: EFP80451.1.
    RefSeqiXP_003324870.1. XM_003324822.1.

    Genome annotation databases

    EnsemblFungiiEFP80451; EFP80451; PGTG_06407.
    GeneIDi10539460.
    KEGGipgr:PGTG_06407.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS178275 Genomic DNA. Translation: EFP80451.1 .
    RefSeqi XP_003324870.1. XM_003324822.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFP80451 ; EFP80451 ; PGTG_06407 .
    GeneIDi 10539460.
    KEGGi pgr:PGTG_06407.

    Phylogenomic databases

    KOi K00772.
    OMAi CEAQLCY.
    OrthoDBi EOG77DJGM.

    Enzyme and pathway databases

    UniPathwayi UPA00904 ; UER00873 .

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    PROSITEi PS01240. PNP_MTAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CRL 75-36-700-3 / race SCCL.

    Entry informationi

    Entry nameiMTAP2_PUCGT
    AccessioniPrimary (citable) accession number: E3K7C1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3