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E3K7C1 (MTAP2_PUCGT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-methyl-5'-thioadenosine phosphorylase 2

EC=2.4.2.28
Alternative name(s):
5'-methylthioadenosine phosphorylase 2
Short name=MTA phosphorylase 2
Short name=MTAP 2
Short name=MTAPase 2
Gene names
ORF Names:PGTG_06407
OrganismPuccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus) [Reference proteome]
Taxonomic identifier418459 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaPucciniomycotinaPucciniomycetesPuccinialesPucciniaceaePuccinia

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates By similarity. HAMAP-Rule MF_03155

Catalytic activity

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_03155

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1. HAMAP-Rule MF_03155

Subunit structure

Homotrimer By similarity. HAMAP-Rule MF_03155

Subcellular location

Cytoplasm By similarity. Nucleus By similarity HAMAP-Rule MF_03155.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Ontologies

Keywords
   Biological processPurine salvage
   Cellular componentCytoplasm
Nucleus
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-methionine salvage from methylthioadenosine

Inferred from electronic annotation. Source: UniProtKB-UniPathway

purine ribonucleoside salvage

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionS-methyl-5-thioadenosine phosphorylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

phosphorylase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290S-methyl-5'-thioadenosine phosphorylase 2 HAMAP-Rule MF_03155
PRO_0000415134

Regions

Region57 – 582Phosphate binding By similarity
Region90 – 912Phosphate binding By similarity
Region209 – 2113Substrate binding By similarity

Sites

Binding site141Phosphate By similarity
Binding site1851Substrate; via amide nitrogen By similarity
Binding site1861Phosphate By similarity
Site1671Important for substrate specificity By similarity
Site2221Important for substrate specificity By similarity

Sequences

Sequence LengthMass (Da)Tools
E3K7C1 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: 5266EC7645DE8A51

FASTA29031,504
        10         20         30         40         50         60 
MTGHAPLVGV IGGSGLYKLE GIEPVESLNI DTPWGRPSSP ITLFKLPSGP VVAFLARHGV 

        70         80         90        100        110        120 
SHQFTPSEVP SRANIAALKK IGCQVIIAFS AVGSLREEIK PRDIVVPSQI IDRTKSAHAM 

       130        140        150        160        170        180 
FGEPFDTELT GLVTKSIKEA VTGFEMNDRI GVHAEKVAIC MEGPAFSTRA ESNMYRMFGG 

       190        200        210        220        230        240 
DIINMSVLPE AKLAREAELS YALIAQITDY DAWRESEEPV TVAEVMATIA ANVSVSNRLT 

       250        260        270        280        290 
LTILDEVHNA VAKGQLKTCK GTMEYSVMTK KEMISEESKK TLSFILPYFS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS178275 Genomic DNA. Translation: EFP80451.1.
RefSeqXP_003324870.1. XM_003324822.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFP80451; EFP80451; PGTG_06407.
GeneID10539460.
KEGGpgr:PGTG_06407.

Phylogenomic databases

OMAMTNHTEA.
OrthoDBEOG77DJGM.

Enzyme and pathway databases

UniPathwayUPA00904; UER00873.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
PROSITEPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTAP2_PUCGT
AccessionPrimary (citable) accession number: E3K7C1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 11, 2011
Last modified: April 16, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways