Skip Header

Contribute Send feedback
Read comments (?) or add your own

E3J1Y4 (E3J1Y4_FRASU) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_00098
EC=6.1.1.10 HAMAP-Rule MF_00098
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_00098
Gene names
Name:metG HAMAP-Rule MF_00098
Ordered Locus Names:FraEuI1c_6105 EMBL ADP84089.1
OrganismFrankia sp. (strain EuI1c) [Complete proteome] [HAMAP]
Taxonomic identifier298654 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). SAAS SAAS023458 HAMAP-Rule MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00098

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00098

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. HAMAP-Rule MF_00098

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif38 – 4811"HIGH" region By similarity HAMAP-Rule MF_00098

Sites

Metal binding1701Zinc By similarity HAMAP-Rule MF_00098
Metal binding1731Zinc By similarity HAMAP-Rule MF_00098
Metal binding1831Zinc By similarity HAMAP-Rule MF_00098
Metal binding1861Zinc By similarity HAMAP-Rule MF_00098
Binding site3641ATP By similarity HAMAP-Rule MF_00098

Sequences

Sequence LengthMass (Da)Tools
E3J1Y4 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: E2DA8DDD689B73DD

FASTA58064,115
        10         20         30         40         50         60 
MLLPGNVKHV TAQINSSSSP GAGDVPGGRP TLVAVAWPYA NGAPHLGHIA GAYLPADIFA 

        70         80         90        100        110        120 
RYQRMAGRRV LMVSGSDAHG TPITVRAEEE GITPQQVVDR YHPIFLDIWE RLGISFDLFT 

       130        140        150        160        170        180 
STRTDNHTAV TQDVFTRLRA AGYISVQETT QFYDPQAGRF LPDRYVVGTC PHCAYPSARG 

       190        200        210        220        230        240 
DQCENCGRTL DPDQLIEPRS KMTGAVPEQR RTSHFFLDLS KLEEPLLAWL ETRTGWRRHV 

       250        260        270        280        290        300 
INWSLQFVKD GLHDRAITRD LDWGVPLPTD ELGPGKRIYV WFEAVIGYLS ASKEWAATIA 

       310        320        330        340        350        360 
GAPEAWRDWW ENPDAAAYYF VGKDNIPFHT VIWPAMLLGY GGLDLPTDVP ANQYVTFGGA 

       370        380        390        400        410        420 
KASKSAGIGR SVPEYLEVLQ PDALRYALAT VLPEQNDTEI SDGDIIRRVN DELIANWGNL 

       430        440        450        460        470        480 
VNRVMAQINK FFDGVLPDVP SPEELGGSHA ELLAKVDEGL ARTAELLEKV ELRAALRAAM 

       490        500        510        520        530        540 
EASGEVNQYL YREEPWKTVK VDRDAAARSL ATSIQAIAGI AVALAPFLPF STERLQDVLG 

       550        560        570        580 
VDLTVWRRQE VPAGRRLDWS AGGLFIKLDP KALDRPEPTA

« Hide

References

[1]"Complete sequence of Frankia sp. EuI1c."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N., Beauchemin N. expand/collapse author list , Sen A., Sur S.A., Gtari M., Wall L., Tisa L., Woyke T.
Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EuI1c EMBL ADP84089.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002299 Genomic DNA. Translation: ADP84089.1.
RefSeqYP_004019959.1. NC_014666.1.

3D structure databases

ProteinModelPortalE3J1Y4.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADP84089; ADP84089; FraEuI1c_6105.
GeneID9976553.
KEGGfri:FraEuI1c_6105.
PATRIC42624527. VBIFraSp2231_6292.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000200402.
KOK01874.

Enzyme and pathway databases

BioCycFSP298654:GHN6-6147-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
HAMAPMF_00098. Met_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE3J1Y4_FRASU
AccessionPrimary (citable) accession number: E3J1Y4
Entry history
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info