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E3IHI3 (E3IHI3_GEOS0) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:GY4MC1_3596 EMBL ADP76235.1
OrganismGeobacillus sp. (strain Y4.1MC1) [Complete proteome] [HAMAP] EMBL ADP76235.1
Taxonomic identifier581103 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047 SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain141 – 346206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding174 – 22956ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3001Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3131Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3131Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E3IHI3 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: 5513AD25E2D0E3DB

FASTA36440,364
        10         20         30         40         50         60 
MKTRVGILYG GKSPEHNVSL STAMAVMNAI DKTKFDVIPI YITTEGQWIK GERLTGQVTE 

        70         80         90        100        110        120 
IKQLQFQSEA KAIIPVALNQ TPTAADGKKQ EEAAVDVIFP LLHGPNGEDG TVQGLLELLN 

       130        140        150        160        170        180 
IPYVGNGVLA SAVGMDKVVM KNLFAQAGLR QAKYVSFTKR EWSKNEEAAY DKVEQALGYP 

       190        200        210        220        230        240 
CFVKPANAGS SVGITKCKRR ADLQAAFAEA FKYDRKIIVE EAIVGREIEI GVIGNDDPIC 

       250        260        270        280        290        300 
SVVGEIVPKK EFYDYQAKYE DGNTELIIPA DVTSEQYETI KQMAITAFKA LDLSGLVRAD 

       310        320        330        340        350        360 
FFLGEDGEVY INEINTMPGF TPYSMFPLLW KHSGISYPEL IERLVQLAIE RHEEKQTITY 


TFEK

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References

[1]"Complete sequence of chromosome of Geobacillus sp. Y4.1MC1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Zhang X., Detter J.C., Han C., Tapia R., Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N., Ovchinnikova G. expand/collapse author list , Brumm P., Mead D., Woyke T.
Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Y4.1MC1 EMBL ADP76235.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002293 Genomic DNA. Translation: ADP76235.1.
RefSeqYP_003990846.1. NC_014650.1.

3D structure databases

ProteinModelPortalE3IHI3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADP76235; ADP76235; GY4MC1_3596.
GeneID9930520.
KEGGgmc:GY4MC1_3596.
PATRIC42637774. VBIGeoSp111282_3968.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.
OMAMAKENIC.

Enzyme and pathway databases

BioCycGSP581103:GHT2-3788-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE3IHI3_GEOS0
AccessionPrimary (citable) accession number: E3IHI3
Entry history
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: May 1, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info