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E3I8H6 (E3I8H6_RHOVT) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase HAMAP-Rule MF_00162

EC=6.3.2.3 HAMAP-Rule MF_00162
Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162
Gene names
Name:gshB HAMAP-Rule MF_00162
Ordered Locus Names:Rvan_1634 EMBL ADP70885.1
OrganismRhodomicrobium vannielii (strain ATCC 17100 / ATH 3.1.1 / DSM 162 / LMG 4299) [Complete proteome] [HAMAP] EMBL ADP70885.1
Taxonomic identifier648757 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesHyphomicrobiaceaeRhodomicrobium

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP-Rule MF_00162

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain125 – 309185ATP-grasp By similarity HAMAP-Rule MF_00162
Nucleotide binding151 – 20757ATP By similarity HAMAP-Rule MF_00162

Sites

Metal binding2801Magnesium or manganese By similarity HAMAP-Rule MF_00162
Metal binding2821Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

Sequence LengthMass (Da)Tools
E3I8H6 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: D0198D80603D3C03

FASTA31835,409
        10         20         30         40         50         60 
MRLKTGVQMD PIEKINIWGD STFALLLEAQ KRQHSIFYYT PDKLILRGGR AFAEGHELRV 

        70         80         90        100        110        120 
HDIEGAHFEL GARQKLALDE LDVIHLRQDP PFDMAYITTT HILDSVHPKT LVVNDPTSVR 

       130        140        150        160        170        180 
NAPEKLFVTQ FPDLMPPTLI ARTLGDVTAF REEFGDIIVK PLYGNGGAAV FRIKPDDTNT 

       190        200        210        220        230        240 
GSLVELFQTV FREPFVVQRY LPDVRAGDKR IILVDGEPVG AINRVPAANE TRSNMHVGGR 

       250        260        270        280        290        300 
PEATELTPRE REICAAIGPE LKKRGLIFVG IDVIGDYLTE INVTSPTGIR EVKRFGGADI 

       310 
AALVWDVVEE KVRSAKVP 

« Hide

References

[1]"Genome sequences of eight morphologically diverse alphaproteobacteria."
US DOE Joint Genome Institute
Brown P.J., Kysela D.T., Buechlein A., Hemmerich C., Brun Y.V.
J. Bacteriol. 193:4567-4568(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17100 / ATH 3.1.1 / DSM 162 / LMG 4299.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002292 Genomic DNA. Translation: ADP70885.1.
RefSeqYP_004011984.1. NC_014664.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADP70885; ADP70885; Rvan_1634.
GeneID9932424.
KEGGrva:Rvan_1634.
PATRIC42670475. VBIRhoVan113057_1718.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000265022.
KOK01920.

Enzyme and pathway databases

BioCycRVAN648757:GHZT-1660-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE3I8H6_RHOVT
AccessionPrimary (citable) accession number: E3I8H6
Entry history
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: February 19, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)