ID   E3GXT0_METFV            Unreviewed;       485 AA.
AC   E3GXT0;
DT   11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT   11-JAN-2011, sequence version 1.
DT   27-MAR-2024, entry version 53.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000256|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000256|HAMAP-Rule:MF_01904};
GN   OrderedLocusNames=Mfer_0309 {ECO:0000313|EMBL:ADP77112.1};
OS   Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanothermaceae; Methanothermus.
OX   NCBI_TaxID=523846 {ECO:0000313|EMBL:ADP77112.1, ECO:0000313|Proteomes:UP000002315};
RN   [1] {ECO:0000313|EMBL:ADP77112.1, ECO:0000313|Proteomes:UP000002315}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43054 / DSM 2088 / JCM 10308 / V24 S
RC   {ECO:0000313|Proteomes:UP000002315};
RX   PubMed=21304736;
RA   Anderson I., Djao O.D., Misra M., Chertkov O., Nolan M., Lucas S.,
RA   Lapidus A., Del Rio T.G., Tice H., Cheng J.F., Tapia R., Han C.,
RA   Goodwin L., Pitluck S., Liolios K., Ivanova N., Mavromatis K.,
RA   Mikhailova N., Pati A., Brambilla E., Chen A., Palaniappan K., Land M.,
RA   Hauser L., Chang Y.J., Jeffries C.D., Sikorski J., Spring S., Rohde M.,
RA   Eichinger K., Huber H., Wirth R., Goker M., Detter J.C., Woyke T.,
RA   Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P.,
RA   Kyrpides N.C.;
RT   "Complete genome sequence of Methanothermus fervidus type strain (V24S).";
RL   Stand. Genomic Sci. 3:315-324(2010).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000256|HAMAP-
CC       Rule:MF_01904}.
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DR   EMBL; CP002278; ADP77112.1; -; Genomic_DNA.
DR   RefSeq; WP_013413390.1; NC_014658.1.
DR   AlphaFoldDB; E3GXT0; -.
DR   STRING; 523846.Mfer_0309; -.
DR   GeneID; 9962024; -.
DR   KEGG; mfv:Mfer_0309; -.
DR   HOGENOM; CLU_517433_0_0_2; -.
DR   OrthoDB; 85849at2157; -.
DR   Proteomes; UP000002315; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   NCBIfam; TIGR02751; PEPCase_arch; 1.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Lyase {ECO:0000256|HAMAP-Rule:MF_01904, ECO:0000313|EMBL:ADP77112.1};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Pyruvate {ECO:0000313|EMBL:ADP77112.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002315}.
SQ   SEQUENCE   485 AA;  56175 MW;  A9E6280A83D9BAE0 CRC64;
     MYIPRCMSTQ HPDNVNPPFF SPNSEIKGED EVTETYYVFS HLGCDEQMWD CEGKEVDNYV
     VKKLLTKYEL FFRENKLGKD VFLTLRVPNP AIERAESKIL IETLESIPRS YDTAKVFYGE
     DIAPIFEVIL PMTTSANDLN RIYYYYKKFV AGKEKMLIRK KDITVKEWIG SFKPKEINVI
     PLFEDYKSML NSDKITENYL RDKNFEYQRV FLARSDPAMN YGMISALLLN KIALMKLDEL
     AEKISIDIYP IIGIGSAPFR GNLKPKTVDQ IIAEYPSVHT FTIQSSFKYD NPPSDVSKAI
     KKLKSKKKKN ADIVDIEKSL DIINKYTEQY QKQITNVASI INRLSRFVPS RRKRKLHIGL
     FGYSRKMGNI VLPRAITFTC VLYSIGVPPE ILGFDALSEK DIDFLSDIYV NFEKDFYDAL
     KYLDWESPLI TDSLKKSVRE HFPEVEEDRK HVNISRKIYH LLATDGTDSI KESILEAANL
     RKFLG
//