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E3GPC0

- E3GPC0_EUBLK

UniProt

E3GPC0 - E3GPC0_EUBLK

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, ELI_3072
Organism
Eubacterium limosum (strain KIST612)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi102 – 1021Magnesium; catalytic By similarityUniRule annotation
Active sitei126 – 1261Proton acceptor By similarityUniRule annotation
Binding sitei153 – 1531Allosteric activator ADP By similarityUniRule annotation
Binding sitei161 – 1611Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei210 – 2101Allosteric activator ADP By similarityUniRule annotation
Binding sitei221 – 2211Substrate By similarityUniRule annotation
Binding sitei242 – 2421Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 722ATP By similarityUniRule annotation
Nucleotide bindingi101 – 1044ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciELIM903814:GH0C-3068-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:ELI_3072Imported
OrganismiEubacterium limosum (strain KIST612)Imported
Taxonomic identifieri903814 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesEubacteriaceaeEubacterium
ProteomesiUP000006873: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 245Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni124 – 1263Substrate binding By similarityUniRule annotation
Regioni168 – 1703Substrate binding By similarityUniRule annotation
Regioni184 – 1863Allosteric activator ADP binding By similarityUniRule annotation
Regioni212 – 2143Allosteric activator ADP binding By similarityUniRule annotation
Regioni248 – 2514Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiMELREGH.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

E3GPC0-1 [UniParc]FASTAAdd to Basket

« Hide

MKIGILTSGG DAPGMNAAIR AVVRTAIYHK IDVYGINRGY AGLLDKDISP    50
LNVYSVADIL QKGGTILRTS RSEFFPTEAG QKRAAEVLRE YGIEHLIIIG 100
GDGSLSGALA LKKLGVSVVG IPGTIDNDFG ASDFTIGFDT AVTTAIEAIG 150
KIRDTTFSHN RINVVQVMGR TCGDIALYAG FSVGAEALII PEIETNLEGI 200
CDRLLAGKDR GKLHSIVMLA EGCGSYRDYC KKIEELTQVT TKGTNLGYIQ 250
RGGSPSHFDR NLASFMGYKA VEAIVNDNTG NVVVQRKGEY IMVPLEEALE 300
TPKAFRKDMY KIAKILSI 318
Length:318
Mass (Da):34,213
Last modified:January 11, 2011 - v1
Checksum:iF1D8463D76CB25F7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002273 Genomic DNA. Translation: ADO38041.1.
RefSeqiYP_003961004.1. NC_014624.2.

Genome annotation databases

EnsemblBacteriaiADO38041; ADO38041; ELI_3072.
GeneIDi9884969.
KEGGielm:ELI_3072.
PATRICi42608983. VBIEubLim172772_2838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002273 Genomic DNA. Translation: ADO38041.1 .
RefSeqi YP_003961004.1. NC_014624.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADO38041 ; ADO38041 ; ELI_3072 .
GeneIDi 9884969.
KEGGi elm:ELI_3072.
PATRICi 42608983. VBIEubLim172772_2838.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.
OMAi MELREGH.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci ELIM903814:GH0C-3068-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Eubacterium limosum (strain KIST612)."
    Roh H., Ko H.-J., Kim D., Choi D.G., Park S., Kim S., Kim K.H., Chang I.S., Choi I.-G.
    Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIST612.
  2. Roh H., Ko H.-J., Kim D., Choi D.G., Park S., Kim S., Kim K.H., Chang I.S., Choi I.-G.
    Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KIST612.

Entry informationi

Entry nameiE3GPC0_EUBLK
AccessioniPrimary (citable) accession number: E3GPC0
Entry historyi
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

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