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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Eubacterium limosum (strain KIST612)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
Metal bindingi102 – 1021Magnesium; catalyticUniRule annotation
Active sitei126 – 1261Proton acceptorUniRule annotation
Binding sitei153 – 1531Allosteric activator ADPUniRule annotation
Binding sitei161 – 1611Substrate; shared with dimeric partnerUniRule annotation
Binding sitei210 – 2101Allosteric activator ADPUniRule annotation
Binding sitei221 – 2211SubstrateUniRule annotation
Binding sitei242 – 2421Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 722ATPUniRule annotation
Nucleotide bindingi101 – 1044ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciELIM903814:GH0C-3068-MONOMER.
UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:ELI_3072Imported
OrganismiEubacterium limosum (strain KIST612)Imported
Taxonomic identifieri903814 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesEubacteriaceaeEubacterium
ProteomesiUP000006873: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 245Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni124 – 1263Substrate bindingUniRule annotation
Regioni168 – 1703Substrate bindingUniRule annotation
Regioni184 – 1863Allosteric activator ADP bindingUniRule annotation
Regioni212 – 2143Allosteric activator ADP bindingUniRule annotation
Regioni248 – 2514Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiCGDIALH.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

E3GPC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGILTSGG DAPGMNAAIR AVVRTAIYHK IDVYGINRGY AGLLDKDISP
60 70 80 90 100
LNVYSVADIL QKGGTILRTS RSEFFPTEAG QKRAAEVLRE YGIEHLIIIG
110 120 130 140 150
GDGSLSGALA LKKLGVSVVG IPGTIDNDFG ASDFTIGFDT AVTTAIEAIG
160 170 180 190 200
KIRDTTFSHN RINVVQVMGR TCGDIALYAG FSVGAEALII PEIETNLEGI
210 220 230 240 250
CDRLLAGKDR GKLHSIVMLA EGCGSYRDYC KKIEELTQVT TKGTNLGYIQ
260 270 280 290 300
RGGSPSHFDR NLASFMGYKA VEAIVNDNTG NVVVQRKGEY IMVPLEEALE
310
TPKAFRKDMY KIAKILSI
Length:318
Mass (Da):34,213
Last modified:January 11, 2011 - v1
Checksum:iF1D8463D76CB25F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002273 Genomic DNA. Translation: ADO38041.1.
RefSeqiWP_013381359.1. NC_014624.2.
YP_003961004.1. NC_014624.2.

Genome annotation databases

EnsemblBacteriaiADO38041; ADO38041; ELI_3072.
GeneIDi9884969.
KEGGielm:ELI_3072.
PATRICi42608983. VBIEubLim172772_2838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002273 Genomic DNA. Translation: ADO38041.1.
RefSeqiWP_013381359.1. NC_014624.2.
YP_003961004.1. NC_014624.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADO38041; ADO38041; ELI_3072.
GeneIDi9884969.
KEGGielm:ELI_3072.
PATRICi42608983. VBIEubLim172772_2838.

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiCGDIALH.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.
BioCyciELIM903814:GH0C-3068-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Eubacterium limosum (strain KIST612)."
    Roh H., Ko H.-J., Kim D., Choi D.G., Park S., Kim S., Kim K.H., Chang I.S., Choi I.-G.
    Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIST612Imported.
  2. Roh H., Ko H.-J., Kim D., Choi D.G., Park S., Kim S., Kim K.H., Chang I.S., Choi I.-G.
    Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KIST612.

Entry informationi

Entry nameiE3GPC0_EUBLK
AccessioniPrimary (citable) accession number: E3GPC0
Entry historyi
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: March 4, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.