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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Eubacterium limosum (strain KIST612)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10ATP; via amide nitrogenUniRule annotation1
Metal bindingi102Magnesium; catalyticUniRule annotation1
Active sitei126Proton acceptorUniRule annotation1
Binding sitei153Allosteric activator ADPUniRule annotation1
Binding sitei161Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei210Allosteric activator ADPUniRule annotation1
Binding sitei221SubstrateUniRule annotation1
Binding sitei242Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi71 – 72ATPUniRule annotation2
Nucleotide bindingi101 – 104ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:ELI_3072Imported
OrganismiEubacterium limosum (strain KIST612)Imported
Taxonomic identifieri903814 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesEubacteriaceaeEubacterium
Proteomesi
  • UP000006873 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi903814.ELI_3072.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 274PFKInterPro annotationAdd BLAST273

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 24Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni124 – 126Substrate bindingUniRule annotation3
Regioni168 – 170Substrate bindingUniRule annotation3
Regioni184 – 186Allosteric activator ADP bindingUniRule annotation3
Regioni212 – 214Allosteric activator ADP bindingUniRule annotation3
Regioni248 – 251Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248870.
KOiK00850.
OMAiGRECGYL.
OrthoDBiPOG091H01AC.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

E3GPC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGILTSGG DAPGMNAAIR AVVRTAIYHK IDVYGINRGY AGLLDKDISP
60 70 80 90 100
LNVYSVADIL QKGGTILRTS RSEFFPTEAG QKRAAEVLRE YGIEHLIIIG
110 120 130 140 150
GDGSLSGALA LKKLGVSVVG IPGTIDNDFG ASDFTIGFDT AVTTAIEAIG
160 170 180 190 200
KIRDTTFSHN RINVVQVMGR TCGDIALYAG FSVGAEALII PEIETNLEGI
210 220 230 240 250
CDRLLAGKDR GKLHSIVMLA EGCGSYRDYC KKIEELTQVT TKGTNLGYIQ
260 270 280 290 300
RGGSPSHFDR NLASFMGYKA VEAIVNDNTG NVVVQRKGEY IMVPLEEALE
310
TPKAFRKDMY KIAKILSI
Length:318
Mass (Da):34,213
Last modified:January 11, 2011 - v1
Checksum:iF1D8463D76CB25F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002273 Genomic DNA. Translation: ADO38041.1.
RefSeqiWP_013381359.1. NC_014624.2.

Genome annotation databases

EnsemblBacteriaiADO38041; ADO38041; ELI_3072.
KEGGielm:ELI_3072.
PATRICi42608983. VBIEubLim172772_2838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002273 Genomic DNA. Translation: ADO38041.1.
RefSeqiWP_013381359.1. NC_014624.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi903814.ELI_3072.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADO38041; ADO38041; ELI_3072.
KEGGielm:ELI_3072.
PATRICi42608983. VBIEubLim172772_2838.

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248870.
KOiK00850.
OMAiGRECGYL.
OrthoDBiPOG091H01AC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiE3GPC0_EUBLK
AccessioniPrimary (citable) accession number: E3GPC0
Entry historyi
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.