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E3GPC0

- E3GPC0_EUBLK

UniProt

E3GPC0 - E3GPC0_EUBLK

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Eubacterium limosum (strain KIST612)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 23 (01 Oct 2014)
      Sequence version 1 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
    Metal bindingi102 – 1021Magnesium; catalyticUniRule annotation
    Active sitei126 – 1261Proton acceptorUniRule annotation
    Binding sitei153 – 1531Allosteric activator ADPUniRule annotation
    Binding sitei161 – 1611Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei210 – 2101Allosteric activator ADPUniRule annotation
    Binding sitei221 – 2211SubstrateUniRule annotation
    Binding sitei242 – 2421Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi71 – 722ATPUniRule annotation
    Nucleotide bindingi101 – 1044ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciELIM903814:GH0C-3068-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:ELI_3072Imported
    OrganismiEubacterium limosum (strain KIST612)Imported
    Taxonomic identifieri903814 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesEubacteriaceaeEubacterium
    ProteomesiUP000006873: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni20 – 245Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni124 – 1263Substrate bindingUniRule annotation
    Regioni168 – 1703Substrate bindingUniRule annotation
    Regioni184 – 1863Allosteric activator ADP bindingUniRule annotation
    Regioni212 – 2143Allosteric activator ADP bindingUniRule annotation
    Regioni248 – 2514Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiMELREGH.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    E3GPC0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIGILTSGG DAPGMNAAIR AVVRTAIYHK IDVYGINRGY AGLLDKDISP    50
    LNVYSVADIL QKGGTILRTS RSEFFPTEAG QKRAAEVLRE YGIEHLIIIG 100
    GDGSLSGALA LKKLGVSVVG IPGTIDNDFG ASDFTIGFDT AVTTAIEAIG 150
    KIRDTTFSHN RINVVQVMGR TCGDIALYAG FSVGAEALII PEIETNLEGI 200
    CDRLLAGKDR GKLHSIVMLA EGCGSYRDYC KKIEELTQVT TKGTNLGYIQ 250
    RGGSPSHFDR NLASFMGYKA VEAIVNDNTG NVVVQRKGEY IMVPLEEALE 300
    TPKAFRKDMY KIAKILSI 318
    Length:318
    Mass (Da):34,213
    Last modified:January 11, 2011 - v1
    Checksum:iF1D8463D76CB25F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002273 Genomic DNA. Translation: ADO38041.1.
    RefSeqiYP_003961004.1. NC_014624.2.

    Genome annotation databases

    EnsemblBacteriaiADO38041; ADO38041; ELI_3072.
    GeneIDi9884969.
    KEGGielm:ELI_3072.
    PATRICi42608983. VBIEubLim172772_2838.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002273 Genomic DNA. Translation: ADO38041.1 .
    RefSeqi YP_003961004.1. NC_014624.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADO38041 ; ADO38041 ; ELI_3072 .
    GeneIDi 9884969.
    KEGGi elm:ELI_3072.
    PATRICi 42608983. VBIEubLim172772_2838.

    Phylogenomic databases

    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi MELREGH.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci ELIM903814:GH0C-3068-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Eubacterium limosum (strain KIST612)."
      Roh H., Ko H.-J., Kim D., Choi D.G., Park S., Kim S., Kim K.H., Chang I.S., Choi I.-G.
      Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KIST612Imported.
    2. Roh H., Ko H.-J., Kim D., Choi D.G., Park S., Kim S., Kim K.H., Chang I.S., Choi I.-G.
      Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: KIST612.

    Entry informationi

    Entry nameiE3GPC0_EUBLK
    AccessioniPrimary (citable) accession number: E3GPC0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: January 11, 2011
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3