E3GCF1 (CPDA_ENTCS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA
Short name=3',5'-cyclic AMP phosphodiesterase
Short name=cAMP phosphodiesterase
EC=3.1.4.17

Gene names

Name:	cpdA
Ordered Locus Names:	Entcl_3882

Organism

Enterobacter cloacae (strain SCF1) [Complete proteome] [HAMAP]

Taxonomic identifier

701347 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter › Enterobacter cloacae complex › Enterobacter cloacae

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes By similarity. HAMAP-Rule MF_00905
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. HAMAP-Rule MF_00905
Cofactor	Binds 2 metal cations per subunit By similarity.
Sequence similarities	Belongs to the cAMP phosphodiesterase class-III family.

Ontologies

Keywords
Ligand	Metal-binding Nucleotide-binding cAMP
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA HAMAP-Rule MF_00905		PRO_0000413367
Regions
	Nucleotide binding	80 – 81	2	cAMP By similarity
Sites
	Metal binding	8	1	Metal cation 1 By similarity
	Metal binding	10	1	Metal cation 1 By similarity
	Metal binding	50	1	Metal cation 1 By similarity
	Metal binding	50	1	Metal cation 2 By similarity
	Metal binding	80	1	Metal cation 2 By similarity
	Metal binding	156	1	Metal cation 2 By similarity
	Metal binding	195	1	Metal cation 2 By similarity
	Metal binding	197	1	Metal cation 1 By similarity
	Binding site	10	1	cAMP By similarity
	Binding site	50	1	cAMP By similarity
	Binding site	197	1	cAMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	E3GCF1 [UniParc]. Last modified January 11, 2011. Version 1. Checksum: CFF0D7D4DDB3AF10	FASTA	278	31,308
	10 20 30 40 50 60 MLLAHISDTH FRSQNHKLYG FIDVNAGNAD VVSQLNGLRE RPDAVVVSGD IVNCGRPEEY 70 80 90 100 110 120 QVARQVLGAL RYPLLLIPGN HDDKACFLEY LRPLCPQLGS DPQNMRYAID DFATRLLFID 130 140 150 160 170 180 SSLAGHAKGW LTDNTVAWLE AQLSDAGDKP TAVFMHHPPL PLGNAQMDPI ACENGHRLLA 190 200 210 220 230 240 LVERFPSLVR IFCGHNHNLT MTQYRQATIA TLPATVHQVP YCHEDTRPYY DMSPPSCLMH 250 260 270 RQVGEQWVSY QHSLAHYAGP WLYDEHISCP TDERRSPC

References

[1]

"Complete sequence of Enterobacter cloacae SCF1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N. , DeAngelis K., Arkin A.P., Chivian D., Edwards B., Woo H., Hazen T.C., Woyke T. Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCF1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002272 Genomic DNA. Translation: ADO50122.1.
RefSeq	YP_003943406.1. NC_014618.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADO50122; ADO50122; Entcl_3882.
GeneID	9908964.
KEGG	esc:Entcl_3882.
PATRIC	42602022. VBIEntClo171306_3959.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000238352.
OMA	QMDRIAC.
Enzyme and pathway databases
BioCyc	ECLO701347:GH9V-3959-MONOMER.
Family and domain databases
HAMAP	MF_00905. cAMP_phophodiest_CpdA.
InterPro	IPR026575. cAMP_Pdiest_CpdA. IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CPDA_ENTCS

Accession

Primary (citable) accession number: E3GCF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program