E3G9V4 (E3G9V4_ENTCS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase RuleBase RU004136
EC=6.3.2.10 RuleBase RU004136

Gene names

Ordered Locus Names:

Entcl_3640

Organism

Enterobacter cloacae (strain SCF1) [Complete proteome] [HAMAP] EMBL ADO49881.1

Taxonomic identifier

701347 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter › Enterobacter cloacae complex › Enterobacter cloacae

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family. RuleBase RU003630

Ontologies

Keywords
Biological process	Cell cycle Cell division RuleBase RU004136 Cell shape Cell wall biogenesis/degradation RuleBase RU004136 Peptidoglycan synthesis RuleBase RU004136
Ligand	ATP-binding RuleBase RU003630 Nucleotide-binding
Molecular function	Ligase RuleBase RU003630 EMBL ADO49881.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	E3G9V4 [UniParc]. Last modified January 11, 2011. Version 1. Checksum: 5CFEDFA9B0454F2B	FASTA	452	47,311
	10 20 30 40 50 60 MIRVTLSQLA DVLQGELHGS DLAVDAVTTD TRKVTAGCLF VALKGERFDA HDFVEQAKEG 70 80 90 100 110 120 GAGALLVSRK LACSLPQIVV KDTRLAFGEL AAWVRQQVPA RVVALTGSSG KTSVKEMTAA 130 140 150 160 170 180 ILGQCGKTLY TAGNLNNDIG VPMTLLRLTR EHEFAVIELG ANHQGEIAWT VSLTRPEAAL 190 200 210 220 230 240 VNNLAAAHLE GFGSLAGVAK AKGEIFTGLP QDGIAILNAD NNDWLNWQSV IGDRQVWRFS 250 260 270 280 290 300 PNAAGSDFTA SDIQVTSGGT AFTLRTPQGS IDVLLPLPGR HNIANALAAA ALAMAVGAGL 310 320 330 340 350 360 DAVKAGLAKL QAVPGRLFPI SLAQHKLLLD DSYNANVGSM TAAAQVLSDM PGYRVLVVGD 370 380 390 400 410 420 MAELGDESEA CHRQVGEAAK AAGIDRVFSV GRLSQLIGAA SGVGEHFADK AALIARLKTA 430 440 450 IEEQQIITIL VKGSRSAAME EVVRALQENG TC

References

[1]

"Complete sequence of Enterobacter cloacae SCF1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N. , DeAngelis K., Arkin A.P., Chivian D., Edwards B., Woo H., Hazen T.C., Woyke T. Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCF1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002272 Genomic DNA. Translation: ADO49881.1.
RefSeq	YP_003943165.1. NC_014618.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADO49881; ADO49881; Entcl_3640.
GeneID	9908718.
KEGG	esc:Entcl_3640.
PATRIC	42601519. VBIEntClo171306_3711.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000268120.
KO	K01929.
OMA	GNRNNEI.
Enzyme and pathway databases
BioCyc	ECLO701347:GH9V-3713-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
PANTHER	PTHR23135:SF3. PTHR23135:SF3. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01143. murF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E3G9V4_ENTCS

Accession

Primary (citable) accession number: E3G9V4

Entry history

Integrated into UniProtKB/TrEMBL:	January 11, 2011
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)