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E3G2D1 (APHA_ENTLS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Class B acid phosphatase

Short name=CBAP
EC=3.1.3.2
Gene names
Name:aphA
Ordered Locus Names:Entcl_4115
OrganismEnterobacter lignolyticus (strain SCF1) [Complete proteome] [HAMAP]
Taxonomic identifier701347 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the class B bacterial acid phosphatase family.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 238212Class B acid phosphatase
PRO_5000650984

Regions

Region138 – 1392Substrate binding By similarity

Sites

Active site701Nucleophile By similarity
Active site721Proton donor By similarity
Metal binding701Magnesium By similarity
Metal binding721Magnesium; via carbonyl oxygen By similarity
Metal binding1931Magnesium By similarity
Binding site1781Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
E3G2D1 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: 88FA28E49C38100B

FASTA23826,372
        10         20         30         40         50         60 
MRKITLALSA FCLLFTLNST VVDAHASSPS PLYPGTNVAK LAEQAPIHWV SVAQIENSLV 

        70         80         90        100        110        120 
GRPPMAVGFD IDDTVLFSSP GFWRGQKTYS PGSDAYLKNP EFWEKMNNGW DEFSIPKEVA 

       130        140        150        160        170        180 
RALISMHVKR GDSIYFITGR SETRTETVSK TLQDDFLIPA DNMNPVIFAG DKAGQNTKTQ 

       190        200        210        220        230 
WLREKNIKMF YGDSDNDITA AHDVGVRGIR VLRASNSTYK PLPMAGAFGE EVIVNSEY 

« Hide

References

[1]"Complete sequence of Enterobacter cloacae SCF1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N. expand/collapse author list , DeAngelis K., Arkin A.P., Chivian D., Edwards B., Woo H., Hazen T.C., Woyke T.
Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SCF1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002272 Genomic DNA. Translation: ADO50348.1.
RefSeqYP_003943632.1. NC_014618.1.

3D structure databases

ProteinModelPortalE3G2D1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADO50348; ADO50348; Entcl_4115.
GeneID9909200.
KEGGesc:Entcl_4115.
PATRIC42602494. VBIEntClo171306_4192.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000270623.
KOK03788.

Enzyme and pathway databases

BioCycECLO701347:GH9V-4196-MONOMER.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01672. AphA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAPHA_ENTLS
AccessionPrimary (citable) accession number: E3G2D1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 11, 2011
Last modified: May 14, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families