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E3G2D1

- APHA_ENTLS

UniProt

E3G2D1 - APHA_ENTLS

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Protein

Class B acid phosphatase

Gene

aphA

Organism
Enterobacter lignolyticus (strain SCF1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701NucleophileBy similarity
Metal bindingi70 – 701MagnesiumBy similarity
Active sitei72 – 721Proton donorBy similarity
Metal bindingi72 – 721Magnesium; via carbonyl oxygenBy similarity
Binding sitei178 – 1781SubstrateBy similarity
Metal bindingi193 – 1931MagnesiumBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECLO701347:GH9V-4196-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseBy similarity (EC:3.1.3.2ImportedBy similarity)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:Entcl_4115
OrganismiEnterobacter lignolyticus (strain SCF1)
Taxonomic identifieri701347 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
ProteomesiUP000006872: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 238212Class B acid phosphataseSequence AnalysisPRO_5000650984Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliE3G2D1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni138 – 1392Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000270623.
KOiK03788.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E3G2D1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKITLALSA FCLLFTLNST VVDAHASSPS PLYPGTNVAK LAEQAPIHWV
60 70 80 90 100
SVAQIENSLV GRPPMAVGFD IDDTVLFSSP GFWRGQKTYS PGSDAYLKNP
110 120 130 140 150
EFWEKMNNGW DEFSIPKEVA RALISMHVKR GDSIYFITGR SETRTETVSK
160 170 180 190 200
TLQDDFLIPA DNMNPVIFAG DKAGQNTKTQ WLREKNIKMF YGDSDNDITA
210 220 230
AHDVGVRGIR VLRASNSTYK PLPMAGAFGE EVIVNSEY
Length:238
Mass (Da):26,372
Last modified:January 11, 2011 - v1
Checksum:i88FA28E49C38100B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002272 Genomic DNA. Translation: ADO50348.1.
RefSeqiYP_003943632.1. NC_014618.1.

Genome annotation databases

EnsemblBacteriaiADO50348; ADO50348; Entcl_4115.
GeneIDi9909200.
KEGGiesc:Entcl_4115.
PATRICi42602494. VBIEntClo171306_4192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002272 Genomic DNA. Translation: ADO50348.1 .
RefSeqi YP_003943632.1. NC_014618.1.

3D structure databases

ProteinModelPortali E3G2D1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADO50348 ; ADO50348 ; Entcl_4115 .
GeneIDi 9909200.
KEGGi esc:Entcl_4115.
PATRICi 42602494. VBIEntClo171306_4192.

Phylogenomic databases

HOGENOMi HOG000270623.
KOi K03788.

Enzyme and pathway databases

BioCyci ECLO701347:GH9V-4196-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCF1Imported.

Entry informationi

Entry nameiAPHA_ENTLS
AccessioniPrimary (citable) accession number: E3G2D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 11, 2011
Last modified: November 26, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3