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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Paenibacillus polymyxa (strain SC2) (Bacillus polymyxa)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotationSAAS annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotationSAAS annotation

Cofactori

FADUniRule annotationSAAS annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16FADUniRule annotation6

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotationImported, Transferase

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

FADUniRule annotationSAAS annotation, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotationSAAS annotation (EC:2.1.1.74UniRule annotationSAAS annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gidImported
ORF Names:PPSC2_09825Imported
OrganismiPaenibacillus polymyxa (strain SC2) (Bacillus polymyxa)Imported
Taxonomic identifieri886882 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus
Proteomesi
  • UP000006868 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi886882.PPSC2_c2082.

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 6 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E3EG21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELQKVTVI GAGLAGSEAA WQIASRGVPV KLYEMRPVVK TPAHHTDKFA
60 70 80 90 100
ELVCSNSLRA NGLTNAVGVL KEEMRILNSL VLSAADRHAV PAGGALAVDR
110 120 130 140 150
DGFSGHITDT LHQHPLIEVV NEELQELPQD GIVVVATGPL TSPALSEQIK
160 170 180 190 200
TLMGEEYFYF YDAAAPIVEK DSIDMSKVYL ASRYDKGEAA YLNCPMNEAE
210 220 230 240 250
FDAFYEALIT AEVAQVKEFE KEIYFEGCMP IEVMMQRGKQ TALFGPMKPV
260 270 280 290 300
GLVNPHTGEL PYAVVQLRQD NAAGTLYNLV GFQTHLKWGE QKRVFSMIPG
310 320 330 340 350
LEEAEFVRYG VMHRNTFINS PQQLHPTYQF KGRSNLFFAG QMTGVEGYVE
360 370 380 390 400
SAASGLLAGI NAARAARGQE MFVFPAETTL GSMARYITTA DFKHFQPMNA
410 420 430 440
NFGLLPKLET RIRNKKEKNE ALANRALESL RGYINQTGVM STEVQA
Length:446
Mass (Da):49,138
Last modified:January 11, 2011 - v1
Checksum:i2DEDEAC8EE3127C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002213 Genomic DNA. Translation: ADO56054.1.
RefSeqiWP_013370668.1. NC_014622.2.

Genome annotation databases

EnsemblBacteriaiADO56054; ADO56054; PPSC2_09825.
GeneIDi25386618.
KEGGippm:PPSC2_09825.
PATRICi42507414. VBIPaePol172748_1988.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002213 Genomic DNA. Translation: ADO56054.1.
RefSeqiWP_013370668.1. NC_014622.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi886882.PPSC2_c2082.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADO56054; ADO56054; PPSC2_09825.
GeneIDi25386618.
KEGGippm:PPSC2_09825.
PATRICi42507414. VBIPaePol172748_1988.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 6 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE3EG21_PAEPS
AccessioniPrimary (citable) accession number: E3EG21
Entry historyi
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.