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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Paenibacillus polymyxa (strain SC2) (Bacillus polymyxa)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotationSAAS annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotationSAAS annotation

Cofactori

FADUniRule annotationSAAS annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166FADUniRule annotation

GO - Molecular functioni

  1. 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-HAMAP
  2. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
  3. methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

FADUniRule annotationSAAS annotation, Flavoprotein

Enzyme and pathway databases

BioCyciPPOL886882:GBY1-2169-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotationSAAS annotation (EC:2.1.1.74UniRule annotationSAAS annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:PPSC2_c2082Imported
OrganismiPaenibacillus polymyxa (strain SC2) (Bacillus polymyxa)Imported
Taxonomic identifieri886882 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus
ProteomesiUP000006868 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E3EG21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELQKVTVI GAGLAGSEAA WQIASRGVPV KLYEMRPVVK TPAHHTDKFA
60 70 80 90 100
ELVCSNSLRA NGLTNAVGVL KEEMRILNSL VLSAADRHAV PAGGALAVDR
110 120 130 140 150
DGFSGHITDT LHQHPLIEVV NEELQELPQD GIVVVATGPL TSPALSEQIK
160 170 180 190 200
TLMGEEYFYF YDAAAPIVEK DSIDMSKVYL ASRYDKGEAA YLNCPMNEAE
210 220 230 240 250
FDAFYEALIT AEVAQVKEFE KEIYFEGCMP IEVMMQRGKQ TALFGPMKPV
260 270 280 290 300
GLVNPHTGEL PYAVVQLRQD NAAGTLYNLV GFQTHLKWGE QKRVFSMIPG
310 320 330 340 350
LEEAEFVRYG VMHRNTFINS PQQLHPTYQF KGRSNLFFAG QMTGVEGYVE
360 370 380 390 400
SAASGLLAGI NAARAARGQE MFVFPAETTL GSMARYITTA DFKHFQPMNA
410 420 430 440
NFGLLPKLET RIRNKKEKNE ALANRALESL RGYINQTGVM STEVQA
Length:446
Mass (Da):49,138
Last modified:January 11, 2011 - v1
Checksum:i2DEDEAC8EE3127C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002213 Genomic DNA. Translation: ADO56054.1.
RefSeqiYP_003946295.1. NC_014622.1.

Genome annotation databases

EnsemblBacteriaiADO56054; ADO56054; PPSC2_c2082.
GeneIDi9850393.
KEGGippm:PPSC2_c2082.
PATRICi42507414. VBIPaePol172748_1988.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002213 Genomic DNA. Translation: ADO56054.1.
RefSeqiYP_003946295.1. NC_014622.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADO56054; ADO56054; PPSC2_c2082.
GeneIDi9850393.
KEGGippm:PPSC2_c2082.
PATRICi42507414. VBIPaePol172748_1988.

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.

Enzyme and pathway databases

BioCyciPPOL886882:GBY1-2169-MONOMER.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Paenibacillus polymyxa SC2, a strain of plant growth-promoting Rhizobacterium with broad-spectrum antimicrobial activity."
    Ma M., Wang C., Ding Y., Li L., Shen D., Jiang X., Guan D., Cao F., Chen H., Feng R., Wang X., Ge Y., Yao L., Bing X., Yang X., Li J., Du B.
    J. Bacteriol. 193:311-312(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC2Imported.

Entry informationi

Entry nameiE3EG21_PAEPS
AccessioniPrimary (citable) accession number: E3EG21
Entry historyi
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: March 4, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.