E3EDT0 (E3EDT0_PAEPS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP-Rule MF_01897
EC=5.99.1.3 HAMAP-Rule MF_01897

Gene names

Name:	gyrA HAMAP-Rule MF_01897
Ordered Locus Names:	PPSC2_c0008 EMBL ADO53996.1

Organism

Paenibacillus polymyxa (strain SC2) (Bacillus polymyxa) [Complete proteome] [HAMAP]

Taxonomic identifier

886882 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Paenibacillus › Paenibacillus polymyxa

Protein attributes

Sequence length	854 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS005743
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP-Rule MF_01897
Ligand	ATP-binding HAMAP-Rule MF_01897 SAAS SAAS013760 DNA-binding SAAS SAAS005743 HAMAP-Rule MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005743 HAMAP-Rule MF_01897
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

	Sequence		Length	Mass (Da)
	E3EDT0 [UniParc]. Last modified January 11, 2011. Version 1. Checksum: C928C8ECA111C3EB	FASTA	854	95,760
	10 20 30 40 50 60 MADQNNSQII NRDIGVEMRE SFMDYAMSII VSRALPDVRD GLKPVHRRIL YAMSELGMSP 70 80 90 100 110 120 DKPYKKSARI VGEVIGKYHP HGDTAVYDTM VRMAQDFSLR YMLVDGHGNF GSVDGDMAAA 130 140 150 160 170 180 MRYTEARLSK IAMEMLRDLN KETVDFMPNY DGEENEPVVL PARYPNLLVN GVGGIAVGMA 190 200 210 220 230 240 TNIPPHNLGE VIDGVQALIE NPDITPMELM DYIQGPDFPT AGYILGRSGI RQAYQTGRGS 250 260 270 280 290 300 VTMRAKTTIE EIGNKARIIV HELPYQVNKA RLVEKIAELV RDKRIEGITD LRDESDRTGM 310 320 330 340 350 360 RIVIELRRDV NPNVVLNNLF KHTAMQSNFG INMLAIVNNE PKILNLKDVL YYYLKHQIEV 370 380 390 400 410 420 IRRRTEFDLK KAEARAHILE GLRIALDHLD EVISLIRSSQ TAEAAREGLI ERFSLTLEQA 430 440 450 460 470 480 QAILDMRLQR LTGLEREKIE NEYNELIQKI MEYREILANE HLVLNIISEE LNELKGRFAD 490 500 510 520 530 540 DRRTEITVGE ESILDEDLIP REDVIITVTH TGYIKRLPVT TYRSQKRGGR GVVGMDTKDE 550 560 570 580 590 600 DFVEHLFITN SHHHLLFFTD KGKVYRIKAY EIPDLSRTAR GTPIINLIQI EQGESINAVI 610 620 630 640 650 660 PIEEFVEDSY LFFATQHGII KKTPLDDYAN IRKGGLIAIN LREDDALIEV KLTDGQQEMI 670 680 690 700 710 720 IGTAQGMSIR FPESDVRSMG RSATGVKGIS LDESDAVIGM DIVNTDLDIL IVTAKGYGKR 730 740 750 760 770 780 TPAVDYRIQS RGGKGIKTIN VTDKNGPVVG LKVVKAEEDL MIITASGTLI RTSMGEISTM 790 800 810 820 830 840 GRNTQGVRLI NIRDDDSVAT VCRANKNEEQ DELLEDLLED GGTGEEPTLS SAEPTLEVNT 850 EDTEGNDSES SEDE

References

[1]

"Complete genome sequence of Paenibacillus polymyxa SC2, a strain of plant growth-promoting Rhizobacterium with broad-spectrum antimicrobial activity." Ma M., Wang C., Ding Y., Li L., Shen D., Jiang X., Guan D., Cao F., Chen H., Feng R., Wang X., Ge Y., Yao L., Bing X., Yang X., Li J., Du B. J. Bacteriol. 193:311-312(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002213 Genomic DNA. Translation: ADO53996.1.
RefSeq	YP_003944237.1. NC_014622.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADO53996; ADO53996; PPSC2_c0008.
GeneID	9848335.
KEGG	ppm:PPSC2_c0008.
PATRIC	42503290. VBIPaePol172748_0009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000076278.
KO	K02469.
OMA	IAQEDVV.
Family and domain databases
Gene3D	3.30.1360.40. 1 hit. 3.90.199.10. 2 hits.
HAMAP	MF_01897. GyrA.
InterPro	IPR024946. Arg_repress_C-like. IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E3EDT0_PAEPS

Accession

Primary (citable) accession number: E3EDT0

Entry history

Integrated into UniProtKB/TrEMBL:	January 11, 2011
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)