UniProtKB - E3ECX2 (E3ECX2_PAEPS)
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Protein
Amidophosphoribosyltransferase
Gene
purF
Organism
Paenibacillus polymyxa (strain SC2) (Bacillus polymyxa)
Status
Functioni
Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation
Catalytic activityi
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation
Cofactori
Protein has several cofactor binding sites:- Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
- [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Amidophosphoribosyltransferase (purF)
- Phosphoribosylamine--glycine ligase (purD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 33 | For GATase activityUniRule annotation | 1 | |
Active sitei | 33 | NucleophileUniRule annotation | 1 | |
Metal bindingi | 267 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 314 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 376 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 377 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 413 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 469 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 472 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- amidophosphoribosyltransferase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniRule
- glutamine metabolic process Source: UniProtKB-KW
- nucleoside metabolic process Source: InterPro
- purine nucleobase biosynthetic process Source: InterPro
Keywordsi
Molecular function | GlycosyltransferaseUniRule annotationImported, Transferase |
Biological process | Purine biosynthesisUniRule annotation |
Ligand | 4Fe-4SUniRule annotation, Iron, Iron-sulfur, MagnesiumUniRule annotation, Metal-binding |
Enzyme and pathway databases
BioCyci | PPOL886882:G1GOE-865-MONOMER. |
UniPathwayi | UPA00074; UER00124. |
Protein family/group databases
MEROPSi | C44.001. |
Names & Taxonomyi
Protein namesi | Recommended name: AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)Short name: ATaseUniRule annotation Alternative name(s): Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation Short name: GPATaseUniRule annotation |
Gene namesi | Name:purFUniRule annotationImported ORF Names:PPSC2_04245Imported |
Organismi | Paenibacillus polymyxa (strain SC2) (Bacillus polymyxa)Imported |
Taxonomic identifieri | 886882 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Paenibacillus › |
Proteomesi |
|
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 250 | Glutamine amidotransferase type-2InterPro annotationAdd BLAST | 218 |
Sequence similaritiesi
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Keywords - Domaini
Glutamine amidotransferaseUniRule annotationPhylogenomic databases
eggNOGi | ENOG4105CBA. Bacteria. COG0034. LUCA. |
HOGENOMi | HOG000033688. |
KOi | K00764. |
OMAi | FRPLCLG. |
OrthoDBi | POG091H0061. |
Family and domain databases
CDDi | cd00715. GPATase_N. 1 hit. cd06223. PRTases_typeI. 1 hit. |
Gene3Di | 3.60.20.10. 3 hits. |
HAMAPi | MF_01931. PurF. 1 hit. |
InterProi | View protein in InterPro IPR017932. GATase_2_dom. IPR029055. Ntn_hydrolases_N. IPR000836. PRibTrfase_dom. IPR029057. PRTase-like. IPR005854. PurF. IPR035584. PurF_N. |
Pfami | View protein in Pfam PF13537. GATase_7. 1 hit. PF00156. Pribosyltran. 1 hit. |
PIRSFi | PIRSF000485. Amd_phspho_trans. 1 hit. |
SUPFAMi | SSF53271. SSF53271. 1 hit. SSF56235. SSF56235. 1 hit. |
TIGRFAMsi | TIGR01134. purF. 1 hit. |
PROSITEi | View protein in PROSITE PS51278. GATASE_TYPE_2. 1 hit. |
i Sequence
Sequence statusi: Complete.
E3ECX2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDEITARTL WTGDYYNEGS GKEGLFDKLK EECGVFGVYR HSDAASLAYY
60 70 80 90 100
GLHALQHRGE ESAGICVSSG DEFHYHRGMG LVKEVFNKDL MASLTGDIAI
110 120 130 140 150
GHVRYSTSGD SKLTNAQPLV FKYRDGDLAV ATNGNIVNAL QIRHELEQGG
160 170 180 190 200
SIFQTTSDTE VVAHLIARSS KDLVEAAKDA LKRIVGGFAF LIMTNDKLLV
210 220 230 240 250
ASDPNGLRPL TMGRLGDAYL FASESCALET IGAELLRDIE PGELLILDKN
260 270 280 290 300
GLHEDRYTED KQRKALCAME YIYFARPDSD LNGANLHAAR KRMGSQLALE
310 320 330 340 350
AFVDADLVTG VPDSSISAAI GYAEQTGIPY ELGMIKNKYT GRTFIQPSQE
360 370 380 390 400
LREQGVKMKL SAVRRVVEGK RVIMIDDSIV RGTTSRRIVN MLRDAGALEV
410 420 430 440 450
HVRITSPPFK NPCFYGIDTP DRRELIASSK TVEEIRQEIN ADSLFFMSAE
460 470 480 490
GLIAAVGGHN EQDYKGGLCL ACFDNDYPTQ VDFKGEEKFG CSC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP002213 Genomic DNA. Translation: ADO54841.1. |
RefSeqi | WP_013369475.1. NC_014622.2. |
Genome annotation databases
EnsemblBacteriai | ADO54841; ADO54841; PPSC2_04245. |
GeneIDi | 25385515. |
KEGGi | ppm:PPSC2_04245. |
PATRICi | fig|886882.15.peg.845. |
Similar proteinsi
Entry informationi
Entry namei | E3ECX2_PAEPS | |
Accessioni | E3ECX2Primary (citable) accession number: E3ECX2 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | January 11, 2011 |
Last sequence update: | January 11, 2011 | |
Last modified: | March 28, 2018 | |
This is version 61 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |