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UniProtKB - E3ECX2 (E3ECX2_PAEPS)

UniProt

E3ECX2 - E3ECX2_PAEPS

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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Paenibacillus polymyxa (strain SC2) (Bacillus polymyxa)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei33 – 331For GATase activityUniRule annotation
Active sitei33 – 331NucleophileUniRule annotation
Metal bindingi267 – 2671Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi314 – 3141MagnesiumUniRule annotation
Metal bindingi376 – 3761MagnesiumUniRule annotation
Metal bindingi377 – 3771MagnesiumUniRule annotation
Metal bindingi413 – 4131Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi469 – 4691Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi472 – 4721Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosyltransferaseUniRule annotationImported, Transferase

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

4Fe-4SUniRule annotation, Iron, Iron-sulfur, MagnesiumUniRule annotation, Metal-binding

Enzyme and pathway databases

BioCyciPPOL886882:GBY1-933-MONOMER.
UniPathwayiUPA00074; UER00124.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Gene namesi
Name:purFUniRule annotationImported
ORF Names:PPSC2_04245Imported
OrganismiPaenibacillus polymyxa (strain SC2) (Bacillus polymyxa)Imported
Taxonomic identifieri886882 [NCBI]
Taxonomic lineageiBacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Paenibacillus ›
Proteomesi
  • UP000006868 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi886882.PPSC2_c0858.

Structurei

3D structure databases

ProteinModelPortaliE3ECX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 250218Glutamine amidotransferase type-2InterPro annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotation

Phylogenomic databases

eggNOGiENOG4105CBA. Bacteria.
COG0034. LUCA.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIRHFGVK.
OrthoDBiPOG091H0061.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E3ECX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDEITARTL WTGDYYNEGS GKEGLFDKLK EECGVFGVYR HSDAASLAYY 
        60         70         80         90        100
GLHALQHRGE ESAGICVSSG DEFHYHRGMG LVKEVFNKDL MASLTGDIAI 
       110        120        130        140        150
GHVRYSTSGD SKLTNAQPLV FKYRDGDLAV ATNGNIVNAL QIRHELEQGG 
       160        170        180        190        200
SIFQTTSDTE VVAHLIARSS KDLVEAAKDA LKRIVGGFAF LIMTNDKLLV 
       210        220        230        240        250
ASDPNGLRPL TMGRLGDAYL FASESCALET IGAELLRDIE PGELLILDKN 
       260        270        280        290        300
GLHEDRYTED KQRKALCAME YIYFARPDSD LNGANLHAAR KRMGSQLALE 
       310        320        330        340        350
AFVDADLVTG VPDSSISAAI GYAEQTGIPY ELGMIKNKYT GRTFIQPSQE 
       360        370        380        390        400
LREQGVKMKL SAVRRVVEGK RVIMIDDSIV RGTTSRRIVN MLRDAGALEV 
       410        420        430        440        450
HVRITSPPFK NPCFYGIDTP DRRELIASSK TVEEIRQEIN ADSLFFMSAE 
       460        470        480        490 
GLIAAVGGHN EQDYKGGLCL ACFDNDYPTQ VDFKGEEKFG CSC
Length:493
Mass (Da):54,175
Last modified:January 11, 2011 - v1
Checksum:iB0E2327A471F1ACA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002213 Genomic DNA. Translation: ADO54841.1.
RefSeqiWP_013369475.1. NC_014622.2.

Genome annotation databases

EnsemblBacteriaiADO54841; ADO54841; PPSC2_04245.
GeneIDi25385515.
KEGGippm:PPSC2_04245.
PATRICi42505074. VBIPaePol172748_0828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002213 Genomic DNA. Translation: ADO54841.1.
RefSeqiWP_013369475.1. NC_014622.2.

3D structure databases

ProteinModelPortaliE3ECX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi886882.PPSC2_c0858.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADO54841; ADO54841; PPSC2_04245.
GeneIDi25385515.
KEGGippm:PPSC2_04245.
PATRICi42505074. VBIPaePol172748_0828.

Phylogenomic databases

eggNOGiENOG4105CBA. Bacteria.
COG0034. LUCA.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIRHFGVK.
OrthoDBiPOG091H0061.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciPPOL886882:GBY1-933-MONOMER.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE3ECX2_PAEPS
AccessioniPrimary (citable) accession number: E3ECX2
Entry historyi
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: September 7, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.