Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

E3ECX2 (E3ECX2_PAEPS) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485

Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Ordered Locus Names:PPSC2_c0858 EMBL ADO54841.1
OrganismPaenibacillus polymyxa (strain SC2) (Bacillus polymyxa) [Complete proteome] [HAMAP] EMBL ADO54841.1
Taxonomic identifier886882 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site331For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2671Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4131Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4691Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4721Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
E3ECX2 [UniParc].

Last modified January 11, 2011. Version 1.
Checksum: B0E2327A471F1ACA

FASTA49354,175
        10         20         30         40         50         60 
MSDEITARTL WTGDYYNEGS GKEGLFDKLK EECGVFGVYR HSDAASLAYY GLHALQHRGE 

        70         80         90        100        110        120 
ESAGICVSSG DEFHYHRGMG LVKEVFNKDL MASLTGDIAI GHVRYSTSGD SKLTNAQPLV 

       130        140        150        160        170        180 
FKYRDGDLAV ATNGNIVNAL QIRHELEQGG SIFQTTSDTE VVAHLIARSS KDLVEAAKDA 

       190        200        210        220        230        240 
LKRIVGGFAF LIMTNDKLLV ASDPNGLRPL TMGRLGDAYL FASESCALET IGAELLRDIE 

       250        260        270        280        290        300 
PGELLILDKN GLHEDRYTED KQRKALCAME YIYFARPDSD LNGANLHAAR KRMGSQLALE 

       310        320        330        340        350        360 
AFVDADLVTG VPDSSISAAI GYAEQTGIPY ELGMIKNKYT GRTFIQPSQE LREQGVKMKL 

       370        380        390        400        410        420 
SAVRRVVEGK RVIMIDDSIV RGTTSRRIVN MLRDAGALEV HVRITSPPFK NPCFYGIDTP 

       430        440        450        460        470        480 
DRRELIASSK TVEEIRQEIN ADSLFFMSAE GLIAAVGGHN EQDYKGGLCL ACFDNDYPTQ 

       490 
VDFKGEEKFG CSC 

« Hide

References

[1]"Complete genome sequence of Paenibacillus polymyxa SC2, a strain of plant growth-promoting Rhizobacterium with broad-spectrum antimicrobial activity."
Ma M., Wang C., Ding Y., Li L., Shen D., Jiang X., Guan D., Cao F., Chen H., Feng R., Wang X., Ge Y., Yao L., Bing X., Yang X., Li J., Du B.
J. Bacteriol. 193:311-312(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC2 EMBL ADO54841.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002213 Genomic DNA. Translation: ADO54841.1.
RefSeqYP_003945082.1. NC_014622.1.

3D structure databases

ProteinModelPortalE3ECX2.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADO54841; ADO54841; PPSC2_c0858.
GeneID9849180.
KEGGppm:PPSC2_c0858.
PATRIC42505074. VBIPaePol172748_0828.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000033688.
KOK00764.
OMASGIPYEM.

Enzyme and pathway databases

BioCycPPOL886882:GBY1-933-MONOMER.
UniPathwayUPA00074; UER00124.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE3ECX2_PAEPS
AccessionPrimary (citable) accession number: E3ECX2
Entry history
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)