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E3DPE1

- E3DPE1_HALPG

UniProt

E3DPE1 - E3DPE1_HALPG

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, Hprae_1576
Organism
Halanaerobium praevalens (strain ATCC 33744 / DSM 2228 / GSL)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi103 – 1031Magnesium; catalytic By similarityUniRule annotation
Active sitei128 – 1281Proton acceptor By similarityUniRule annotation
Binding sitei155 – 1551Allosteric activator ADP By similarityUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei212 – 2121Allosteric activator ADP By similarityUniRule annotation
Binding sitei223 – 2231Substrate By similarityUniRule annotation
Binding sitei256 – 2561Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarityUniRule annotation
Nucleotide bindingi102 – 1054ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPRA572479:GLDS-1622-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Hprae_1576Imported
OrganismiHalanaerobium praevalens (strain ATCC 33744 / DSM 2228 / GSL)Imported
Taxonomic identifieri572479 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalanaerobium
ProteomesiUP000006866: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotationSAAS annotations

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni126 – 1283Substrate binding By similarityUniRule annotation
Regioni170 – 1723Substrate binding By similarityUniRule annotation
Regioni186 – 1883Allosteric activator ADP binding By similarityUniRule annotation
Regioni214 – 2163Allosteric activator ADP binding By similarityUniRule annotation
Regioni262 – 2654Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

KOiK00850.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E3DPE1-1 [UniParc]FASTAAdd to Basket

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MKKIAVLTSG GDAPGMNPAI RSVVRTAIYH GLEVVGIRHG YKGLIEKDFF    50
PMKRGSVADI VQRGGTILLS ARCEEFKTEA GRKKAYANMQ EEGIDGLVVI 100
GGDGSLRGVQ KINAESDIKA VGIPGTIDND LACTDYTIGV DTAMNTIVEA 150
INKIRDTATS HERTFVIECM GRDSGYLALM TGLAGGAEYI LVPEIDFTPQ 200
QVCDKITQGF KRGKLHSLIL IAEGVKAPFA DLTEYHGSPG MAMGDLIREK 250
TGMETRVTIL GHLQRGGSPT ATDRIVASRM AAKAVEILLD GQRDKMINYL 300
NNSIGVCEIE NAIKKTETIN MDIYNLANIL SM 332
Length:332
Mass (Da):35,824
Last modified:January 11, 2011 - v1
Checksum:iB5F5F07AA87ABDBA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002175 Genomic DNA. Translation: ADO77703.1.
RefSeqiWP_014553723.1. NC_017455.1.
YP_005836863.1. NC_017455.1.

Genome annotation databases

EnsemblBacteriaiADO77703; ADO77703; Hprae_1576.
GeneIDi12443552.
KEGGihpk:Hprae_1576.
PATRICi43056473. VBIHalPra106773_1596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002175 Genomic DNA. Translation: ADO77703.1 .
RefSeqi WP_014553723.1. NC_017455.1.
YP_005836863.1. NC_017455.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADO77703 ; ADO77703 ; Hprae_1576 .
GeneIDi 12443552.
KEGGi hpk:Hprae_1576.
PATRICi 43056473. VBIHalPra106773_1596.

Phylogenomic databases

KOi K00850.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci HPRA572479:GLDS-1622-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33744 / DSM 2228 / GSL.

Entry informationi

Entry nameiE3DPE1_HALPG
AccessioniPrimary (citable) accession number: E3DPE1
Entry historyi
Integrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: September 3, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

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