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Protein

Endonuclease 8

Gene

nei

Organism
Shigella dysenteriae 1617
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNAUniRule annotation1
Active sitei3Proton donorUniRule annotation1
Active sitei53Proton donor; for beta-elimination activityUniRule annotation1
Binding sitei70DNAUniRule annotation1
Binding sitei125DNAUniRule annotation1
Binding sitei169DNAUniRule annotation1
Active sitei253Proton donor; for delta-elimination activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, EndonucleaseImported, GlycosidaseUniRule annotationSAAS annotationImported, Hydrolase, LyaseUniRule annotationSAAS annotationImported, Multifunctional enzymeUniRule annotationSAAS annotation, Nuclease
Biological processDNA damage, DNA repairUniRule annotationSAAS annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8UniRule annotation
Alternative name(s):
DNA glycosylase/AP lyase NeiUniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
DNA-(apurinic or apyrimidinic site) lyase NeiUniRule annotation
Endonuclease VIIIUniRule annotation
Gene namesi
Name:neiUniRule annotation
ORF Names:Asd1617_00819Imported, SD1617_2869Imported
OrganismiShigella dysenteriae 1617Imported
Taxonomic identifieri754093 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000031647 Componenti: Chromosome
  • UP000003738 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 111FPG_CATInterPro annotationAdd BLAST110
Domaini229 – 263FPG-typeInterPro annotationAdd BLAST35

Sequence similaritiesi

Belongs to the FPG family.UniRule annotationSAAS annotation

Keywords - Domaini

Zinc-fingerUniRule annotationSAAS annotation

Phylogenomic databases

KOiK05522

Family and domain databases

Gene3Di3.20.190.10, 1 hit
HAMAPiMF_01253 Endonuclease_8, 1 hit
InterProiView protein in InterPro
IPR015886 DNA_glyclase/AP_lyase_DNA-bd
IPR015887 DNA_glyclase_Znf_dom_DNA_BS
IPR023713 Endonuclease-VIII
IPR012319 FPG_cat
IPR035937 MutM-like_N-ter
IPR010979 Ribosomal_S13-like_H2TH
IPR000214 Znf_DNA_glyclase/AP_lyase
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF01149 Fapy_DNA_glyco, 1 hit
PF06831 H2TH, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
SMARTiView protein in SMART
SM00898 Fapy_DNA_glyco, 1 hit
SM01232 H2TH, 1 hit
SUPFAMiSSF46946 SSF46946, 1 hit
SSF81624 SSF81624, 1 hit
PROSITEiView protein in PROSITE
PS51068 FPG_CAT, 1 hit
PS01242 ZF_FPG_1, 1 hit
PS51066 ZF_FPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E2XAE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEGPEIRRA ADNLEAAIKG KPLTDVWFAF PQLKTYQSQL IGQHVTHVET
60 70 80 90 100
RGKALLTHFS NDLTLYSHNQ LYGVWRVVDT GEESQTTRVL RVKLQTADKT
110 120 130 140 150
ILLYSASDIE MLTPEQLTMH PFLQRVGPDV LDPNLTPEVV KERLLSPRFR
160 170 180 190 200
NRQFAGLLLD QAFLAGLGNY LRVEILWQVG LTGNHKAKDL NAAQLDALAH
210 220 230 240 250
ALLDIPRFSY ATRGQVDENK HHGALFRFKV FHRDGELCER CGGIIEKTTL
260
SSRPFYWCPG CQH
Length:263
Mass (Da):29,841
Last modified:January 11, 2011 - v1
Checksum:i89E58FDDF6E0707E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP006736 Genomic DNA Translation: AHA63646.1
ADUT01000026 Genomic DNA Translation: EFP71248.1

Genome annotation databases

EnsemblBacteriaiAHA63646; AHA63646; Asd1617_00819
EFP71248; EFP71248; SD1617_2869
KEGGisdz:Asd1617_00819
PATRICifig|754093.4.peg.805

Similar proteinsi

Entry informationi

Entry nameiE2XAE0_SHIDY
AccessioniPrimary (citable) accession number: E2XAE0
Entry historyiIntegrated into UniProtKB/TrEMBL: January 11, 2011
Last sequence update: January 11, 2011
Last modified: June 20, 2018
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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