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Protein

Wee1-like protein kinase 2

Gene

WEE2

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei244ATPPROSITE-ProRule annotation1
Active sitei342Proton acceptorPROSITE-ProRule annotation1
Metal bindingi347Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi384Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 229ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Gene namesi
Name:WEE2
Synonyms:WEE1B
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004095251 – 567Wee1-like protein kinase 2Add BLAST567

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation leads to increase its activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiE2RSS3.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000005790.

Structurei

3D structure databases

ProteinModelPortaliE2RSS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini215 – 494Protein kinasePROSITE-ProRule annotationAdd BLAST280

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili497 – 523Sequence analysisAdd BLAST27

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi174 – 176Nuclear localization signalBy similarity3
Motifi318 – 332Nuclear export signalBy similarityAdd BLAST15

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
InParanoidiE2RSS3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E2RSS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDSSINKEL KQKLNVSYCE EESESEGQKE APESRETQSQ TPDWAEGQES
60 70 80 90 100
EAKFTPPRTP SSSIHGVGTF EEKDKMSPDQ ALRTPGPGFH KCPGTPAQPD
110 120 130 140 150
SRSEVVHCES PYTPKSLLSQ SVISSTEKLP SRGSKHLRFT PVPFVDEMTS
160 170 180 190 200
SALVNINPFT PESYRKQFLR SNGKRKTRGD LEEADPGEGK VEQGLPAKRC
210 220 230 240 250
VLRETNMASR YEKEFLEVEK IGVGEFGTVY KCIKRLDGCV YAIKRSMKPV
260 270 280 290 300
AGLSNENLAL HEVYAHAVLG HHPHVVRYYS AWAEDDHMII QNEYCNGGSL
310 320 330 340 350
QTAISENTKS GNHFPELKLK DILLQISLGL KYIHNSGMVH LDIKPSNIFI
360 370 380 390 400
CHKMQCDSPV VPEEIENEAD WFLSANVMYK IAGDLGHVTS ISKPKVEEGD
410 420 430 440 450
SRFLANEILQ EDYQHLPKAD IFALGLTIAV AAGAESLPAN GAKWHHIREG
460 470 480 490 500
NLPDIPQKLS EEFHNLLKNM IHPDPSERPS AAGLARSRVL RPSLRKAEEL
510 520 530 540 550
QQQLNLEKSK TATLERELRE AQQAWSPQEE HSDPGVSGTP TGSRCTKRPV
560
GGKSAKSSSF TCGKSSP
Length:567
Mass (Da):62,857
Last modified:November 30, 2010 - v1
Checksum:i97C445AF4E232354
GO

Cross-referencesi

3D structure databases

ProteinModelPortaliE2RSS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000005790.

Proteomic databases

PaxDbiE2RSS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
InParanoidiE2RSS3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWEE2_CANLF
AccessioniPrimary (citable) accession number: E2RSS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: November 30, 2010
Last modified: May 11, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.