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Protein

Ubiquitin carboxyl-terminal hydrolase 37

Gene

USP37

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-630 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei352NucleophilePROSITE-ProRule annotation1
Active sitei908Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-CFA-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.053.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 37 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 37
Ubiquitin thioesterase 37
Ubiquitin-specific-processing protease 37
Gene namesi
Name:USP37
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 37

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004126451 – 981Ubiquitin carboxyl-terminal hydrolase 37Add BLAST981

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170PhosphoserineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei630Phosphoserine; by CDK2By similarity1
Modified residuei652PhosphoserineBy similarity1
Modified residuei654PhosphoserineBy similarity1
Modified residuei772PhosphoserineBy similarity1

Post-translational modificationi

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the APC(CDH1) complex during late mitosis, leading to its degradation. Able to mediate auto-deubiquitination (By similarity).By similarity
Phosphorylated at Ser-630 by CDK2 during G1/S phase but not during mitosis; phosphorylation at Ser-630 is required for deubiquitinase activity. Also polyubiquitinated during early G1 phase, without leading to degradation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiE2RK09.

Interactioni

Subunit structurei

Interacts with FZR1/CDH1.By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000021817.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini343 – 953USPAdd BLAST611
Domaini706 – 725UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini808 – 827UIM 2PROSITE-ProRule annotationAdd BLAST20
Domaini830 – 849UIM 3PROSITE-ProRule annotationAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 34KEN box 1By similarity3
Motifi71 – 79D-box 1By similarity9
Motifi96 – 105D-box 2By similarity10
Motifi160 – 168D-box 3By similarity9
Motifi223 – 225KEN box 2By similarity3
Motifi784 – 786KEN box 3By similarity3

Domaini

The KEN box 3 is required for interaction with FZR1/CDH1 and is essential for APC(CDH1)-mediated ubiquitination.By similarity

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 3 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1868. Eukaryota.
ENOG410XP8T. LUCA.
GeneTreeiENSGT00440000033542.
InParanoidiE2RK09.
KOiK11850.
OMAiWLQQYDM.

Family and domain databases

CDDicd13312. PH_USP37_like. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR003903. UIM_dom.
IPR032069. USP37-like_PH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF16674. UCH_N. 1 hit.
PF02809. UIM. 3 hits.
[Graphical view]
SMARTiSM00726. UIM. 4 hits.
[Graphical view]
PROSITEiPS50330. UIM. 3 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E2RK09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPLKIHGPI RIRSMQTGIT KWKEGSFEIV EKENKVSLVV HYNTGGIPRI
60 70 80 90 100
FQLSHNIKNV VLRPSGAKQS RLMLTLQDNS FLSIDKVPSK DAEEMRLFLD
110 120 130 140 150
AVHQNRLHAA MKPSQGSGSF GAILGSRTSQ KETNRQLSYS DNQASSKRGS
160 170 180 190 200
LETKDDIPFR KVLGNPGRGS IKTATGSGIT VTRTIPSLTS ASTPLRSGLL
210 220 230 240 250
ENRTEKRKRM LSSGSELNED YPKENDSSSN NKAMTDPSRK YLTSSREKQL
260 270 280 290 300
SLKQSEENRT SGLLPLQSSS FYGSRTGSKD YSSGSTNLDR TNVSGQTPSA
310 320 330 340 350
KRSLGFLPQP APLSVKKLRC NQDYTGWNKP RVPLSSHQQQ QLQGFSNLGN
360 370 380 390 400
TCYMNAILQS LFSLQSFAND LLKQGIPWKK IPLNALIRRF AHLLVKKDIC
410 420 430 440 450
NSETKKDLLK KVKNAISATA ERFSGYMQND AHEFLSQCLD QLKEDMEKLN
460 470 480 490 500
KTWKTEPVPG EENSPDISAT RVYTCPVITN LEFEVQHSII CKACGEIIPK
510 520 530 540 550
REQFNDLSID LPRRKKPLPP RSIQDSLDLF FRAEELEYSC EKCGGKCALV
560 570 580 590 600
RHKFNRLPRI LILHLKRYSF NVTLSLNNKI GQQVIIPRYL TLSSHCTENT
610 620 630 640 650
KPPFTLGWSA HMAISRPLKA SQMVNSCITS PSTPSKNFTF KSKSSLALCL
660 670 680 690 700
DSDSEDELKR SVALSQRLCE MSGCEQQQDD LEKDSKPCRI EPDKSELENS
710 720 730 740 750
GFDGMSEEEL LAAVLEMSKR EASPTLSHED DDKPTSSPDT GFAEDDIQEM
760 770 780 790 800
PENQDPVETE KPKTVTEPDP ASFTEITKDC DENKENKTPE GSQGEVDWLQ
810 820 830 840 850
QYDMEREREE QELQQALAQS LQEQEAWEQK EDDDLKRATE LSLQEFNNSF
860 870 880 890 900
LDSLGSDEDS GNEDVLDMEY TEAEAEELKR NAETGNLPHS YRLISVVSHI
910 920 930 940 950
GSTSSSGHYI SDVYDIKKQA WFTYNDLEVS KIQEASVQSD RDRSGYIFFY
960 970 980
MHKEIFDELL ETEKNSQALS MEVGKTTRQA L
Length:981
Mass (Da):110,453
Last modified:November 30, 2010 - v1
Checksum:iEBF79BF01F784AE5
GO

Sequence databases

RefSeqiNP_001300791.1. NM_001313862.1.
XP_013966369.1. XM_014110894.1.
XP_013966370.1. XM_014110895.1.
XP_013966371.1. XM_014110896.1.
XP_545643.2. XM_545643.5.

Genome annotation databases

EnsembliENSCAFT00000023494; ENSCAFP00000021817; ENSCAFG00000014781.
GeneIDi488523.
KEGGicfa:488523.

Cross-referencesi

Sequence databases

RefSeqiNP_001300791.1. NM_001313862.1.
XP_013966369.1. XM_014110894.1.
XP_013966370.1. XM_014110895.1.
XP_013966371.1. XM_014110896.1.
XP_545643.2. XM_545643.5.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000021817.

Protein family/group databases

MEROPSiC19.053.

Proteomic databases

PaxDbiE2RK09.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000023494; ENSCAFP00000021817; ENSCAFG00000014781.
GeneIDi488523.
KEGGicfa:488523.

Organism-specific databases

CTDi57695.

Phylogenomic databases

eggNOGiKOG1868. Eukaryota.
ENOG410XP8T. LUCA.
GeneTreeiENSGT00440000033542.
InParanoidiE2RK09.
KOiK11850.
OMAiWLQQYDM.

Enzyme and pathway databases

ReactomeiR-CFA-5689880. Ub-specific processing proteases.

Family and domain databases

CDDicd13312. PH_USP37_like. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR003903. UIM_dom.
IPR032069. USP37-like_PH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF16674. UCH_N. 1 hit.
PF02809. UIM. 3 hits.
[Graphical view]
SMARTiSM00726. UIM. 4 hits.
[Graphical view]
PROSITEiPS50330. UIM. 3 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP37_CANLF
AccessioniPrimary (citable) accession number: E2RK09
Secondary accession number(s): F1PGV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: November 30, 2010
Last modified: November 2, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.