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Protein

Amidophosphoribosyltransferase

Gene

PPAT

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (PPAT)
  2. Trifunctional purine biosynthetic protein adenosine-3 (LOC100856570), Trifunctional purine biosynthetic protein adenosine-3 (LOC100856570)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12For GATase activityUniRule annotation1
Metal bindingi280Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi426Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi503Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi506Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotation, Transferase
Biological processPurine biosynthesisUniRule annotation
LigandIron, Iron-sulfurUniRule annotation, Metal-binding

Enzyme and pathway databases

ReactomeiR-CFA-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Gene namesi
Name:PPATImported
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)Imported
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 13

Organism-specific databases

VGNCiVGNC:44839. PPAT.

PTM / Processingi

Proteomic databases

PaxDbiE2RF42.

Expressioni

Gene expression databases

BgeeiENSCAFG00000002217.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000003241.

Structurei

3D structure databases

ProteinModelPortaliE2RF42.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 261Glutamine amidotransferase type-2InterPro annotationAdd BLAST250

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0572. Eukaryota.
COG0034. LUCA.
GeneTreeiENSGT00390000003428.
InParanoidiE2RF42.
KOiK00764.
OMAiFRPLCLG.
OrthoDBiEOG091G06F7.
TreeFamiTF106370.

Family and domain databases

CDDicd00715. GPATase_N. 1 hit.
cd06223. PRTases_typeI. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
IPR035584. PurF_N.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

E2RF42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELEESGMRE ECGVFGCIAS GEWPTQLDVP HVITLGLVGL QHRGQESAGI
60 70 80 90 100
VTSDGNSVPT FKTHKGMGLV NHVFTQDNLK KLYISNLGIG HTRYATTGNC
110 120 130 140 150
ELENCQPFVV ETLHGKIAVA HNGELVNAAQ LRKKLLRHGI GLSTSSDSEM
160 170 180 190 200
ITQLLAYTPP QEQDGTPDWV ARIKNLMKEA PTAYSLLIMH RDVIYAVRDP
210 220 230 240 250
YGNRPLCIGS LIPVSDINDK EKKSSETEGW VVSSESCSFL SIGAKYYREV
260 270 280 290 300
LPGEIVEITK RSVQTLDIIP RSEGNPMAFC IFEYVYFARP DSIFEDQMVY
310 320 330 340 350
TVRYRCGQQL AIEAPVDADL VSTVPESATP AALGYAGKCG LPYVEVLCKN
360 370 380 390 400
RYVGRTFIQP NMRLRQLGVA KKFGVLSDNF KGKRIVLVDD SIVRGNTISP
410 420 430 440 450
IIKLLKESGA KEVHIRVASP PVRYPCFMGI NIPTKEELIA NKPEFEHLAK
460 470 480 490 500
YLGANSVIYL SVGGLVSSVQ EGIKFKKQKV EKQDIMIQEN GNGLECFEKN
510
GHCTACLTGK YPVELEW
Length:517
Mass (Da):57,180
Last modified:November 30, 2010 - v1
Checksum:iE50414AFE99AC372
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEX03009089 Genomic DNA. No translation available.
AAEX03009090 Genomic DNA. No translation available.
RefSeqiXP_854170.1. XM_849077.4.

Genome annotation databases

EnsembliENSCAFT00000003495; ENSCAFP00000003241; ENSCAFG00000002217.
GeneIDi611407.
KEGGicfa:611407.

Similar proteinsi

Entry informationi

Entry nameiE2RF42_CANLF
AccessioniPrimary (citable) accession number: E2RF42
Entry historyiIntegrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: March 28, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported