Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calmegin

Gene

CLGN

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calmegin
Gene namesi
Name:CLGN
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componentsi: Chromosome 19, Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 480LumenalSequence analysisAdd BLAST451
Transmembranei481 – 501HelicalSequence analysisAdd BLAST21
Topological domaini502 – 620CytoplasmicSequence analysisAdd BLAST119

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000042862830 – 620CalmeginAdd BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei138N6-acetyllysineBy similarity1
Disulfide bondi161 ↔ 195By similarity
Disulfide bondi361 ↔ 365By similarity
Modified residuei570PhosphoserineBy similarity1
Modified residuei586PhosphoserineBy similarity1
Modified residuei589PhosphoserineBy similarity1
Modified residuei591PhosphoserineBy similarity1
Modified residuei601PhosphoserineBy similarity1
Modified residuei604PhosphoserineBy similarity1
Modified residuei611PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiE2RA18.

Interactioni

Subunit structurei

Interacts with PDILT. Interacts with ADAM2 (By similarity). Interacts with PPIB.By similarity1 Publication

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000033194.

Structurei

Secondary structure

1620
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi345 – 348Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ICIX-ray1.70C327-360[»]
SMRiE2RA18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati277 – 2901-1Add BLAST14
Repeati294 – 3071-2Add BLAST14
Repeati313 – 3261-3Add BLAST14
Repeati332 – 3451-4Add BLAST14
Repeati349 – 3622-1Add BLAST14
Repeati366 – 3792-2Add BLAST14
Repeati380 – 3932-3Add BLAST14
Repeati394 – 4072-4Add BLAST14

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni327 – 360Interaction with PPIBAdd BLAST34

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
InParanoidiE2RA18.
OMAiPACRIGC.
OrthoDBiEOG091G04SS.
TreeFamiTF300618.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 2 hits.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E2RA18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPWSTVIS MHFQSFWLCL GLLFISVNAE FMDDDVEMED FDENSEEIDV
60 70 80 90 100
NEGELPSEIN YKTPQPMGEV YFTETFDSGR LAGWVLSKAK KDDTDAEISI
110 120 130 140 150
YDGRWEIEEL KENRVPGDRG LVLKSRAKHH AIAAVLAKPF IFADKPLIVQ
160 170 180 190 200
YEVNFQDGID CGGAYIKLLA DTDGLNLENF YDKTSYTIMF GPDKCGEDYK
210 220 230 240 250
LHFIFRHKHP KTGVFEEKHA KPPDVDLKKF FTDRKTHLYT LVMNPDDTFE
260 270 280 290 300
VLIDQVVVNQ GSLLEDVVPP INPPKEIEDP SDKKPDEWDE RAKIPDPSAV
310 320 330 340 350
KPEDWDESEP AQIEDLSVVK PDGWLDDEPK FIPDPNAEKP DDWNEDMDGE
360 370 380 390 400
WEAPRISNPA CRIGCGEWSP PMIDNPKYKG VWRPPMIDNP NYQGIWSPRK
410 420 430 440 450
IPNPDYFEDD HPFLLTSFRA LGLELWSMTS NIYFDNFIIC SEKETADRWA
460 470 480 490 500
ADGWGVKILV ANANEPGIFK QLMAAAEERP WLWLIYFVTA GLPIALIASF
510 520 530 540 550
CWPRKVKKKY EDSEYKKTDI CKPQTKGALE QEVKEKKAAL EKPVDLEEEK
560 570 580 590 600
KQSDGETVEK EEEAEPEEKS EEEIEIIEGQ EEGNKSNKSG SEDEMKEADE
610 620
STGSGDGPVK SVRKRRVRKE
Length:620
Mass (Da):70,900
Last modified:October 31, 2012 - v2
Checksum:iA1481CDA17B91E05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEX03011717 Genomic DNA. No translation available.
RefSeqiXP_533285.3. XM_533285.5.

Genome annotation databases

EnsembliENSCAFT00000037651; ENSCAFP00000033194; ENSCAFG00000003696.
GeneIDi476076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEX03011717 Genomic DNA. No translation available.
RefSeqiXP_533285.3. XM_533285.5.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ICIX-ray1.70C327-360[»]
SMRiE2RA18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000033194.

Proteomic databases

PaxDbiE2RA18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000037651; ENSCAFP00000033194; ENSCAFG00000003696.
GeneIDi476076.

Organism-specific databases

CTDi1047.

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
InParanoidiE2RA18.
OMAiPACRIGC.
OrthoDBiEOG091G04SS.
TreeFamiTF300618.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 2 hits.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLGN_CANLF
AccessioniPrimary (citable) accession number: E2RA18
Secondary accession number(s): D9N169
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: October 31, 2012
Last modified: November 30, 2016
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.