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Protein

Calmegin

Gene

CLGN

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calmegin
Gene namesi
Name:CLGN
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componentsi: Chromosome 19, Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 480451LumenalSequence analysisAdd
BLAST
Transmembranei481 – 50121HelicalSequence analysisAdd
BLAST
Topological domaini502 – 620119CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 620591CalmeginPRO_0000428628Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei138 – 1381N6-acetyllysineBy similarity
Disulfide bondi161 ↔ 195By similarity
Disulfide bondi361 ↔ 365By similarity
Modified residuei570 – 5701PhosphoserineBy similarity
Modified residuei586 – 5861PhosphoserineBy similarity
Modified residuei589 – 5891PhosphoserineBy similarity
Modified residuei591 – 5911PhosphoserineBy similarity
Modified residuei601 – 6011PhosphoserineBy similarity
Modified residuei604 – 6041PhosphoserineBy similarity
Modified residuei611 – 6111PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiE2RA18.

Interactioni

Subunit structurei

Interacts with PDILT. Interacts with ADAM2 (By similarity). Interacts with PPIB.By similarity1 Publication

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000033194.

Structurei

Secondary structure

1
620
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi345 – 3484Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ICIX-ray1.70C327-360[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati277 – 290141-1Add
BLAST
Repeati294 – 307141-2Add
BLAST
Repeati313 – 326141-3Add
BLAST
Repeati332 – 345141-4Add
BLAST
Repeati349 – 362142-1Add
BLAST
Repeati366 – 379142-2Add
BLAST
Repeati380 – 393142-3Add
BLAST
Repeati394 – 407142-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni327 – 36034Interaction with PPIBAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
InParanoidiE2RA18.
OMAiPACRIGC.
OrthoDBiEOG77126Z.
TreeFamiTF300618.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 2 hits.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E2RA18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPWSTVIS MHFQSFWLCL GLLFISVNAE FMDDDVEMED FDENSEEIDV
60 70 80 90 100
NEGELPSEIN YKTPQPMGEV YFTETFDSGR LAGWVLSKAK KDDTDAEISI
110 120 130 140 150
YDGRWEIEEL KENRVPGDRG LVLKSRAKHH AIAAVLAKPF IFADKPLIVQ
160 170 180 190 200
YEVNFQDGID CGGAYIKLLA DTDGLNLENF YDKTSYTIMF GPDKCGEDYK
210 220 230 240 250
LHFIFRHKHP KTGVFEEKHA KPPDVDLKKF FTDRKTHLYT LVMNPDDTFE
260 270 280 290 300
VLIDQVVVNQ GSLLEDVVPP INPPKEIEDP SDKKPDEWDE RAKIPDPSAV
310 320 330 340 350
KPEDWDESEP AQIEDLSVVK PDGWLDDEPK FIPDPNAEKP DDWNEDMDGE
360 370 380 390 400
WEAPRISNPA CRIGCGEWSP PMIDNPKYKG VWRPPMIDNP NYQGIWSPRK
410 420 430 440 450
IPNPDYFEDD HPFLLTSFRA LGLELWSMTS NIYFDNFIIC SEKETADRWA
460 470 480 490 500
ADGWGVKILV ANANEPGIFK QLMAAAEERP WLWLIYFVTA GLPIALIASF
510 520 530 540 550
CWPRKVKKKY EDSEYKKTDI CKPQTKGALE QEVKEKKAAL EKPVDLEEEK
560 570 580 590 600
KQSDGETVEK EEEAEPEEKS EEEIEIIEGQ EEGNKSNKSG SEDEMKEADE
610 620
STGSGDGPVK SVRKRRVRKE
Length:620
Mass (Da):70,900
Last modified:October 31, 2012 - v2
Checksum:iA1481CDA17B91E05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEX03011717 Genomic DNA. No translation available.
RefSeqiXP_533285.3. XM_533285.5.

Genome annotation databases

EnsembliENSCAFT00000037651; ENSCAFP00000033194; ENSCAFG00000003696.
GeneIDi476076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEX03011717 Genomic DNA. No translation available.
RefSeqiXP_533285.3. XM_533285.5.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ICIX-ray1.70C327-360[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000033194.

Proteomic databases

PaxDbiE2RA18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000037651; ENSCAFP00000033194; ENSCAFG00000003696.
GeneIDi476076.

Organism-specific databases

CTDi1047.

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
InParanoidiE2RA18.
OMAiPACRIGC.
OrthoDBiEOG77126Z.
TreeFamiTF300618.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 2 hits.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence, comparative analysis and haplotype structure of the domestic dog."
    Lindblad-Toh K., Wade C.M., Mikkelsen T.S., Karlsson E.K., Jaffe D.B., Kamal M., Clamp M., Chang J.L., Kulbokas E.J. III, Zody M.C., Mauceli E., Xie X., Breen M., Wayne R.K., Ostrander E.A., Ponting C.P., Galibert F., Smith D.R.
    , deJong P.J., Kirkness E.F., Alvarez P., Biagi T., Brockman W., Butler J., Chin C.-W., Cook A., Cuff J., Daly M.J., DeCaprio D., Gnerre S., Grabherr M., Kellis M., Kleber M., Bardeleben C., Goodstadt L., Heger A., Hitte C., Kim L., Koepfli K.-P., Parker H.G., Pollinger J.P., Searle S.M.J., Sutter N.B., Thomas R., Webber C., Baldwin J., Abebe A., Abouelleil A., Aftuck L., Ait-Zahra M., Aldredge T., Allen N., An P., Anderson S., Antoine C., Arachchi H., Aslam A., Ayotte L., Bachantsang P., Barry A., Bayul T., Benamara M., Berlin A., Bessette D., Blitshteyn B., Bloom T., Blye J., Boguslavskiy L., Bonnet C., Boukhgalter B., Brown A., Cahill P., Calixte N., Camarata J., Cheshatsang Y., Chu J., Citroen M., Collymore A., Cooke P., Dawoe T., Daza R., Decktor K., DeGray S., Dhargay N., Dooley K., Dooley K., Dorje P., Dorjee K., Dorris L., Duffey N., Dupes A., Egbiremolen O., Elong R., Falk J., Farina A., Faro S., Ferguson D., Ferreira P., Fisher S., FitzGerald M., Foley K., Foley C., Franke A., Friedrich D., Gage D., Garber M., Gearin G., Giannoukos G., Goode T., Goyette A., Graham J., Grandbois E., Gyaltsen K., Hafez N., Hagopian D., Hagos B., Hall J., Healy C., Hegarty R., Honan T., Horn A., Houde N., Hughes L., Hunnicutt L., Husby M., Jester B., Jones C., Kamat A., Kanga B., Kells C., Khazanovich D., Kieu A.C., Kisner P., Kumar M., Lance K., Landers T., Lara M., Lee W., Leger J.-P., Lennon N., Leuper L., LeVine S., Liu J., Liu X., Lokyitsang Y., Lokyitsang T., Lui A., Macdonald J., Major J., Marabella R., Maru K., Matthews C., McDonough S., Mehta T., Meldrim J., Melnikov A., Meneus L., Mihalev A., Mihova T., Miller K., Mittelman R., Mlenga V., Mulrain L., Munson G., Navidi A., Naylor J., Nguyen T., Nguyen N., Nguyen C., Nguyen T., Nicol R., Norbu N., Norbu C., Novod N., Nyima T., Olandt P., O'Neill B., O'Neill K., Osman S., Oyono L., Patti C., Perrin D., Phunkhang P., Pierre F., Priest M., Rachupka A., Raghuraman S., Rameau R., Ray V., Raymond C., Rege F., Rise C., Rogers J., Rogov P., Sahalie J., Settipalli S., Sharpe T., Shea T., Sheehan M., Sherpa N., Shi J., Shih D., Sloan J., Smith C., Sparrow T., Stalker J., Stange-Thomann N., Stavropoulos S., Stone C., Stone S., Sykes S., Tchuinga P., Tenzing P., Tesfaye S., Thoulutsang D., Thoulutsang Y., Topham K., Topping I., Tsamla T., Vassiliev H., Venkataraman V., Vo A., Wangchuk T., Wangdi T., Weiand M., Wilkinson J., Wilson A., Yadav S., Yang S., Yang X., Young G., Yu Q., Zainoun J., Zembek L., Zimmer A., Lander E.S.
    Nature 438:803-819(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Boxer.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 327-360 IN COMPLEX WITH PPIB.

Entry informationi

Entry nameiCLGN_CANLF
AccessioniPrimary (citable) accession number: E2RA18
Secondary accession number(s): D9N169
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: October 31, 2012
Last modified: June 8, 2016
This is version 43 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.