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Protein

Phosphoenolpyruvate carboxylase

Gene

ppc

Organism
Escherichia coli
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.UniRule annotationSAAS annotation

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381UniRule annotation
Active sitei546 – 5461UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, PyruvateImported

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylaseUniRule annotationSAAS annotation (EC:4.1.1.31UniRule annotationSAAS annotation)
Short name:
PEPCUniRule annotation
Short name:
PEPCaseUniRule annotation
Gene namesi
Name:ppcUniRule annotationImported
ORF Names:AC789_1c43500Imported, BN17_39251Imported, HUS2011_4726Imported, HW43_01420Imported, IY32_18940Imported, JD73_06060Imported, PU06_06535Imported, PU15_13305Imported, PU21_16325Imported, PU33_05885Imported, PU38_20930Imported, PU53_18745Imported, PU71_21385Imported, PU73_20650Imported, UC21_12250Imported, UN86_23480Imported, UN87_11540Imported, UN88_00685Imported, UN89_20190Imported, UN90_13395Imported, UN91_00375Imported, UN92_17420Imported, UN95_24605Imported, VP68_17810Imported, VP69_05050Imported
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000030783 Componenti: Chromosome UP000032738 Componenti: Chromosome UP000031522 Componenti: Chromosome

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi511145.b3956.

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 1 family.UniRule annotation

Phylogenomic databases

KOiK01595.
OMAiCGMGVIA.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1.
InterProiIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E2QIY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEQYSALRS NVSMLGKVLG ETIKDALGEH ILERVETIRK LSKSSRAGND
60 70 80 90 100
ANRQELLTTL QNLSNDELLP VARAFSQFLN LANTAEQYHS ISPKGEAASN
110 120 130 140 150
PEVIARTLRK LKNQPELSED TIKKAVESLS LELVLTAHPT EITRRTLIHK
160 170 180 190 200
MVEVNACLKQ LDNKDIADYE HNQLMRRLRQ LIAQSWHTDE IRKLRPSPVD
210 220 230 240 250
EAKWGFAVVE NSLWQGVPNY LRELNEQLEE NLGYKLPVEF VPVRFTSWMG
260 270 280 290 300
GDRDGNPNVT ADITRHVLLL SRWKATDLFL KDIQVLVSEL SMVEATPELL
310 320 330 340 350
ALVGEEGAAE PYRYLMKNLR SRLMATQAWL EARLKGEELP KPEGLLTQNE
360 370 380 390 400
ELWEPLYACY QSLQACGMGI IANGDLLDTL RRVKCFGVPL VRIDIRQEST
410 420 430 440 450
RHTEALGELT RYLGIGDYES WSEADKQAFL IRELNSKRPL LPRNWQPSAE
460 470 480 490 500
TREVLDTCQV IAEAPQGSIA AYVISMAKTP SDVLAVHLLL KEAGIGFAMP
510 520 530 540 550
VAPLFETLDD LNNANDVMTQ LLNIDWYRGL IQGKQMVMIG YSDSAKDAGV
560 570 580 590 600
MAASWAQYQA QDALIKTCEK AGIELTLFHG RGGSIGRGGA PAHAALLSQP
610 620 630 640 650
PGSLKGGLRV TEQGEMIRFK YGLPEITVSS LSLYTGAILE ANLLPPPEPK
660 670 680 690 700
ESWRRIMDEL SVISCDLYRG YVRENKDFVP YFRSATPEQE LGKLPLGSRP
710 720 730 740 750
AKRRPTGGVE SLRAIPWIFA WTQNRLMLPA WLGAGTALQK VVEDGKQSEL
760 770 780 790 800
EAMCRDWPFF STRLGMLEMV FAKADLWLAE YYDQRLVDKA LWPLGKELRN
810 820 830 840 850
LQEEDIKVVL AIANDSHLMA DLPWIAESIQ LRNIYTDPLN VLQAELLHRS
860 870 880
RQAEKEGQEP DPRVEQALMV TIAGIAAGMR NTG
Length:883
Mass (Da):99,077
Last modified:November 30, 2010 - v1
Checksum:iC66E18268EB19EFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP009106 Genomic DNA. Translation: AIZ85153.1.
CP010315 Genomic DNA. Translation: AJE58518.1.
HE964769 Genomic DNA. Translation: CCJ46600.1.
HF572917 Genomic DNA. Translation: CCQ31604.2.
JPQG01000001 Genomic DNA. Translation: KFD78693.1.
JUBT01000062 Genomic DNA. Translation: KHG91794.1.
JUBV01000111 Genomic DNA. Translation: KHG99292.1.
JUCW01000021 Genomic DNA. Translation: KHI36097.1.
JUDH01000036 Genomic DNA. Translation: KHI93387.1.
JUDN01000035 Genomic DNA. Translation: KHJ20479.1.
JWZA01000040 Genomic DNA. Translation: KIG50033.1.
JWZF01000081 Genomic DNA. Translation: KIG77690.1.
JWZL01000020 Genomic DNA. Translation: KIH03375.1.
JPPP01000036 Genomic DNA. Translation: KIQ44902.1.
JZDU01000119 Genomic DNA. Translation: KJW25933.1.
JZDW01000012 Genomic DNA. Translation: KJW31853.1.
JZDV01000068 Genomic DNA. Translation: KJW33050.1.
JZDX01000103 Genomic DNA. Translation: KJW39150.1.
JZDY01000098 Genomic DNA. Translation: KJW49030.1.
JZDZ01000016 Genomic DNA. Translation: KJW54553.1.
JZEA01000170 Genomic DNA. Translation: KJW56728.1.
JZED01000160 Genomic DNA. Translation: KJW71564.1.
JYKT01000067 Genomic DNA. Translation: KJY11447.1.
LARR01000360 Genomic DNA. Translation: KKB19538.1.
LARS01000066 Genomic DNA. Translation: KKB20120.1.
RefSeqiWP_001005579.1. NZ_JUKA01000041.1.

Genome annotation databases

EnsemblBacteriaiAHM30566; AHM30566; BU34_18140.
AHM36674; AHM36674; CF57_29160.
AHM41273; AHM41273; CF61_29855.
AIZ80600; AIZ80600; HW42_01315.
AIZ85153; AIZ85153; HW43_01420.
CAP78417; CAP78417; LF82_1696.
EYB38381; EYB38381; BU70_24520.
EYB46321; EYB46321; BU69_09450.
EYB54837; EYB54837; BU65_04945.
EYB57972; EYB57972; BU66_07000.
EYB59945; EYB59945; BU67_03315.
KDM69695; KDM69695; DA88_29135.
KDM85044; KDM85044; DC22_24145.
KDO88313; KDO88313; DO98_06020.
KDP16079; KDP16079; EP08_05610.
KEO95706; KEO95706; EH66_22180.
KEP01453; KEP01453; EH65_20460.
KEP03625; KEP03625; EH64_07840.
KEP08351; KEP08351; EH62_00800.
KEP21613; KEP21613; EH61_21055.
KFD78693; KFD78693; JD73_06060.
KFF39197; KFF39197; BC97_0205620.
KFF54047; KFF54047; BC99_0307475.
KFH77277; KFH77277; GR03_21735.
KFH98867; KFH98867; GR02_13690.
KFV23914; KFV23914; GS40_00985.
KFV27719; KFV27719; GS38_19340.
KFV28398; KFV28398; GS37_03980.
KFV35873; KFV35873; GS39_19560.
KHG72094; KHG72094; PU77_19955.
KHG76140; KHG76140; PU76_22450.
KHG78989; KHG78989; PU75_16000.
KHG84869; KHG84869; PU74_24105.
KHG91794; KHG91794; PU73_20650.
KHG99292; KHG99292; PU71_21385.
KHH00227; KHH00227; PU72_06995.
KHH15189; KHH15189; PU63_21720.
KHH19584; KHH19584; PU67_10375.
KHH27055; KHH27055; PU61_21800.
KHH30637; KHH30637; PU60_22535.
KHH33505; KHH33505; PU62_01755.
KHH37515; KHH37515; PU59_24695.
KHH43852; KHH43852; PU58_16585.
KHH51110; KHH51110; PU56_18665.
KHH55214; KHH55214; PU57_12685.
KHH60460; KHH60460; PU55_16125.
KHH68238; KHH68238; PU54_04740.
KHH75249; KHH75249; PU49_24405.
KHH77452; KHH77452; PU50_14965.
KHH84259; KHH84259; PU51_05215.
KHH89428; KHH89428; PU48_26470.
KHI00845; KHI00845; PU44_17525.
KHI31285; KHI31285; PU32_23270.
KHI32235; KHI32235; PU34_06795.
KHI36097; KHI36097; PU33_05885.
KHI42686; KHI42686; PU27_22270.
KHI46943; KHI46943; PU31_02640.
KHI54152; KHI54152; PU24_13140.
KHI60132; KHI60132; PU22_13580.
KHI65527; KHI65527; PU20_21260.
KHI68852; KHI68852; PU19_21800.
KHI78148; KHI78148; PU16_13595.
KHI85109; KHI85109; PU14_21810.
KHI93387; KHI93387; PU15_13305.
KHI96831; KHI96831; PU12_13765.
KHJ00977; KHJ00977; PU11_11675.
KHJ04697; KHJ04697; PU08_24460.
KHJ15295; KHJ15295; PU10_01905.
KHJ20479; KHJ20479; PU06_06535.
KHJ20616; KHJ20616; PU03_22935.
KHO59313; KHO59313; RT53_08050.
KEGGielf:LF82_1696.
PATRICi48658118. VBIEscCol74806_4046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP009106 Genomic DNA. Translation: AIZ85153.1.
CP010315 Genomic DNA. Translation: AJE58518.1.
HE964769 Genomic DNA. Translation: CCJ46600.1.
HF572917 Genomic DNA. Translation: CCQ31604.2.
JPQG01000001 Genomic DNA. Translation: KFD78693.1.
JUBT01000062 Genomic DNA. Translation: KHG91794.1.
JUBV01000111 Genomic DNA. Translation: KHG99292.1.
JUCW01000021 Genomic DNA. Translation: KHI36097.1.
JUDH01000036 Genomic DNA. Translation: KHI93387.1.
JUDN01000035 Genomic DNA. Translation: KHJ20479.1.
JWZA01000040 Genomic DNA. Translation: KIG50033.1.
JWZF01000081 Genomic DNA. Translation: KIG77690.1.
JWZL01000020 Genomic DNA. Translation: KIH03375.1.
JPPP01000036 Genomic DNA. Translation: KIQ44902.1.
JZDU01000119 Genomic DNA. Translation: KJW25933.1.
JZDW01000012 Genomic DNA. Translation: KJW31853.1.
JZDV01000068 Genomic DNA. Translation: KJW33050.1.
JZDX01000103 Genomic DNA. Translation: KJW39150.1.
JZDY01000098 Genomic DNA. Translation: KJW49030.1.
JZDZ01000016 Genomic DNA. Translation: KJW54553.1.
JZEA01000170 Genomic DNA. Translation: KJW56728.1.
JZED01000160 Genomic DNA. Translation: KJW71564.1.
JYKT01000067 Genomic DNA. Translation: KJY11447.1.
LARR01000360 Genomic DNA. Translation: KKB19538.1.
LARS01000066 Genomic DNA. Translation: KKB20120.1.
RefSeqiWP_001005579.1. NZ_JUKA01000041.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b3956.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAHM30566; AHM30566; BU34_18140.
AHM36674; AHM36674; CF57_29160.
AHM41273; AHM41273; CF61_29855.
AIZ80600; AIZ80600; HW42_01315.
AIZ85153; AIZ85153; HW43_01420.
CAP78417; CAP78417; LF82_1696.
EYB38381; EYB38381; BU70_24520.
EYB46321; EYB46321; BU69_09450.
EYB54837; EYB54837; BU65_04945.
EYB57972; EYB57972; BU66_07000.
EYB59945; EYB59945; BU67_03315.
KDM69695; KDM69695; DA88_29135.
KDM85044; KDM85044; DC22_24145.
KDO88313; KDO88313; DO98_06020.
KDP16079; KDP16079; EP08_05610.
KEO95706; KEO95706; EH66_22180.
KEP01453; KEP01453; EH65_20460.
KEP03625; KEP03625; EH64_07840.
KEP08351; KEP08351; EH62_00800.
KEP21613; KEP21613; EH61_21055.
KFD78693; KFD78693; JD73_06060.
KFF39197; KFF39197; BC97_0205620.
KFF54047; KFF54047; BC99_0307475.
KFH77277; KFH77277; GR03_21735.
KFH98867; KFH98867; GR02_13690.
KFV23914; KFV23914; GS40_00985.
KFV27719; KFV27719; GS38_19340.
KFV28398; KFV28398; GS37_03980.
KFV35873; KFV35873; GS39_19560.
KHG72094; KHG72094; PU77_19955.
KHG76140; KHG76140; PU76_22450.
KHG78989; KHG78989; PU75_16000.
KHG84869; KHG84869; PU74_24105.
KHG91794; KHG91794; PU73_20650.
KHG99292; KHG99292; PU71_21385.
KHH00227; KHH00227; PU72_06995.
KHH15189; KHH15189; PU63_21720.
KHH19584; KHH19584; PU67_10375.
KHH27055; KHH27055; PU61_21800.
KHH30637; KHH30637; PU60_22535.
KHH33505; KHH33505; PU62_01755.
KHH37515; KHH37515; PU59_24695.
KHH43852; KHH43852; PU58_16585.
KHH51110; KHH51110; PU56_18665.
KHH55214; KHH55214; PU57_12685.
KHH60460; KHH60460; PU55_16125.
KHH68238; KHH68238; PU54_04740.
KHH75249; KHH75249; PU49_24405.
KHH77452; KHH77452; PU50_14965.
KHH84259; KHH84259; PU51_05215.
KHH89428; KHH89428; PU48_26470.
KHI00845; KHI00845; PU44_17525.
KHI31285; KHI31285; PU32_23270.
KHI32235; KHI32235; PU34_06795.
KHI36097; KHI36097; PU33_05885.
KHI42686; KHI42686; PU27_22270.
KHI46943; KHI46943; PU31_02640.
KHI54152; KHI54152; PU24_13140.
KHI60132; KHI60132; PU22_13580.
KHI65527; KHI65527; PU20_21260.
KHI68852; KHI68852; PU19_21800.
KHI78148; KHI78148; PU16_13595.
KHI85109; KHI85109; PU14_21810.
KHI93387; KHI93387; PU15_13305.
KHI96831; KHI96831; PU12_13765.
KHJ00977; KHJ00977; PU11_11675.
KHJ04697; KHJ04697; PU08_24460.
KHJ15295; KHJ15295; PU10_01905.
KHJ20479; KHJ20479; PU06_06535.
KHJ20616; KHJ20616; PU03_22935.
KHO59313; KHO59313; RT53_08050.
KEGGielf:LF82_1696.
PATRICi48658118. VBIEscCol74806_4046.

Phylogenomic databases

KOiK01595.
OMAiCGMGVIA.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1.
InterProiIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of avian pathogenic Escherichia coli serogroup O78 genes associated with pathogenesis in poultry."
    Dziva F., Hauser H., Connor T., Van Diemen P.M., Prescott G., Langridge G.C., Eckert S., Chaudhuri R.R., Ewers C., Mellata M., Curtiss III R., Dougan G., Wieler L., Thomson N.R., Pickard D.J., Stevens M.P.
    Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Aslett M.
    Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: IMT2125Imported.
  3. "Sequencing and functional annotation of avian pathogenic Escherichia coli serogroup O78 strains reveal the evolution of E. coli lineages pathogenic for poultry via distinct mechanisms."
    Dziva F., Hauser H., Connor T.R., van Diemen P.M., Prescott G., Langridge G.C., Eckert S., Chaudhuri R.R., Ewers C., Mellata M., Mukhopadhyay S., Curtiss R., Dougan G., Wieler L.H., Thomson N.R., Pickard D.J., Stevens M.P.
    Infect. Immun. 81:838-849(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: IMT2125Imported.
  4. "Evolution of german Escherichia coli HUSEC/EHEC epidemic isolate HUSEC2011 in relation to putative ancestor HUSEC41."
    Kuenne C., Billion A., Fritzenwanker M., Chakraborty T.
    Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: HUSEC2011Imported.
  5. "Comparative Genomic Analysis of an Enterotoxigenic E. coli O157 Lineage from Australian Pigs."
    Wyrsch E., Roy Chowdhury P., Abraham S., Santos J., Darling A., Charles I., Chapman T., Djordjevic S.
    Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: O157 SvETECImported.
  6. "Prevalence of subtilase cytotoxin-encoding subAB variants among Shiga toxin-producing Escherichia coli strains isolated from wild ruminants and sheep differs from that of cattle and pigs and is predominated by the new allelic variant subAB2-2."
    Nuesch-Inderbinen M.T., Funk J., Cernela N., Tasara T., Klumpp J., Schmidt H., Stephan R.
    Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 48Imported.
  7. "Whole-genome sequencing effectively predicts antimicrobial resistance phenotypes of E. coli."
    Tyson G.H., McDermott P.F., Li C., Tadesse D.A., Mukherjee S., Bodeis-Jones S., Kabera C., Gaines S.A., Loneragan G.H., Edrington T.S., Torrence M., Harhay D.M., Zhao S.
    Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: CVM N33561PSImported, CVM N33707PSImported, CVM N33825PSImported, CVM N34352PSImported, CVM N34577PSImported, CVM N36601PSImported, CVM N38823PSImported and CVM N38833PSImported.
  8. "Genome sequencing and comparative genomics provides insights on the evolutionary dynamics and pathogenic potential of different H-serotypes of Shiga toxin-producing Escherichia coli O104."
    Yan X., Fratamico P.M., Bono J.L., Baranzoni G.M., Chen C.Y.
    BMC Microbiol. 15:83-83(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 94-3024Imported.
  9. "Characterization of enteropathogenic and Shiga toxin-producing Escherichia coli in cattle and deer in a shared agroecosystem."
    Singh P., Sha Q., Lacher D.W., Valle J.D., Mosci R.E., Moore J.A., Scribner K.T., Manning S.D.
    Front. Cell. Infect. Microbiol. 5:29-29(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: TW18703Imported and TW18710Imported.
  10. "Genomic Avenue to Avian Colisepticemia."
    Huja S., Oren Y., Trost E., Brzuszkiewicz E., Biran D., Blom J., Goesmann A., Gottschalk G., Hacker J., Ron E.Z., Dobrindt U.
    MBio 6:e01681-14(2015)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 789Imported.
  11. "Genome sequences of 11 non-O157 Shiga Toxin-Producing Escherichia coli strains."
    Gabrielsen C., Afset J.E., Drablos F.
    Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: St. Olav17Imported and St. Olav39Imported.
  12. "Genome sequences of 11 non-O157 Shiga Toxin-Producing Escherichia coli strains."
    Gabrielsen C., Drablos F., Afset J.E.
    Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: St. Olav157Imported, St. Olav172Imported, St. Olav173Imported, St. Olav174Imported, St. Olav179Imported, St. Olav40Imported and St. Olav63Imported.

Entry informationi

Entry nameiE2QIY7_ECOLX
AccessioniPrimary (citable) accession number: E2QIY7
Entry historyi
Integrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: June 24, 2015
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.