E2IUB0 (CASS_KALDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cycloartenol synthase Short name=KdCAS EC=5.4.99.8
Organism	Kalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum)
Taxonomic identifier	23013 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Saxifragales › Crassulaceae › Kalanchoe

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oxidosqualene cyclase which catalyzes the conversion of oxidosqualene to cycloartenol. Ref.1
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = cycloartenol. Ref.1
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Expressed in all leaf tissues. Ref.1
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 5 PFTB repeats.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
Molecular function	Isomerase
Gene Ontology (GO)
Biological_process	steroid biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cycloartenol synthase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	Cycloartenol synthase		PRO_0000418479
Regions
	Transmembrane	609 – 629	21	Helical; Potential
	Repeat	148 – 189	42	PFTB 1
	Repeat	513 – 558	46	PFTB 2
	Repeat	590 – 630	41	PFTB 3
	Repeat	639 – 680	42	PFTB 4
	Repeat	701 – 742	42	PFTB 5

Sequences

	Sequence		Length	Mass (Da)
	E2IUB0 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: 9A59D94C1072CF6A	FASTA	764	86,531
	10 20 30 40 50 60 MWKLKIADAG GSQWLRSVNN HIGRQIWDFD PALGSPEELA QIEDARDNFA RHRFDKKHSA 70 80 90 100 110 120 DLLMRFQLTK ENPQSDLLPK VNIGKIEDIT EDAVTNTLRR AINFHSTTQA HDGHWPGDYG 130 140 150 160 170 180 GPLFLMPGLV ITLSITGALN AVLSKEHKKE MCRYLYNHQN EDGGWGLHIE GPSTMFGSVL 190 200 210 220 230 240 NYVTLRLLGE DVNGGDGEIE RARKWILDHG GATAITSWGK MWLSVLGVFE WCGNNPLPPE 250 260 270 280 290 300 MWLFPYYLPV HPGRMWCHCR MVYLPMSYLY GKRFVGPITP TVLSLRKELF TVPYHEIDWN 310 320 330 340 350 360 EARSLCAKED LYYPHPVVQD ILWATLHKVV EPVLLNWPGK KLREKALCSA IEHIHYEDEN 370 380 390 400 410 420 TRYICIGPVN KVLNMLCCWV EDPNSEAFKL HIPRLYDYLW IAEDGMKMQG YNGSQLWDTA 430 440 450 460 470 480 FSVQAIVATK LVEEFSSTIS KAHEFMKNSQ VLEDYPGDLS YWYRHISKGA WPFSTADHGW 490 500 510 520 530 540 PISDCTAEGL KVVLKLSQFP AELVGAPLSA KLVYNAVNVI LSLQNIDGGF ATYELTRSYS 550 560 570 580 590 600 WMELLNPAET FGDIVIDYPY VECTSAALQS LVLFKKLHPE HRKEEVELCI KKAAAFIEKI 610 620 630 640 650 660 QESDGSWYGS WAVCFTYGTW FGVLGLVAAG RNYKNSPSIR KACDFLLSKQ LASGGWGESY 670 680 690 700 710 720 LSCQNKVYTN IPGGRSHVVN TGWAMLALIG AGQAERDPVP LHRAAKFLIE SQLENGDFPQ 730 740 750 760 QEIMGVFNKN CMISYAAYRN IFPIWALGEY RCKVLNASRG QMKT

References

[1]

"Cloning and characterization of oxidosqualene cyclases from Kalanchoe daigremontiana: enzymes catalyzing up to 10 rearrangement steps yielding friedelin and other triterpenoids." Wang Z., Yeats T., Han H., Jetter R. J. Biol. Chem. 285:29703-29712(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HM623872 mRNA. Translation: ADK35127.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 3 hits. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASS_KALDA

Accession

Primary (citable) accession number: E2IUB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2012
Last sequence update:	November 30, 2010
Last modified:	October 3, 2012
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program