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E2IUA9

- LUPS_KALDA

UniProt

E2IUA9 - LUPS_KALDA

Protein

Lupeol synthase

Gene
N/A
Organism
Kalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 14 (01 Oct 2014)
      Sequence version 1 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Oxidosqualene cyclase that generates lupeol, a triterpenoid product. Lupeol is probably required to coat the leaf exterior as a defense compound against pathogens or herbivores.1 Publication

    Catalytic activityi

    (3S)-2,3-epoxy-2,3-dihydrosqualene = lupeol.1 Publication

    GO - Molecular functioni

    1. intramolecular transferase activity Source: UniProtKB

    GO - Biological processi

    1. triterpenoid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Isomerase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lupeol synthase (EC:5.4.99.41)
    Short name:
    KdLUS
    OrganismiKalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum)
    Taxonomic identifieri23013 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeSaxifragalesCrassulaceaeKalanchoe

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 765765Lupeol synthasePRO_0000418481Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed only in the epidermal cells on both sides of the leaf and not in internal leaf tissues.1 Publication

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati148 – 18942PFTB 1Add
    BLAST
    Repeati640 – 68142PFTB 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the terpene cyclase/mutase family.Curated
    Contains 2 PFTB repeats.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di1.50.10.20. 2 hits.
    InterProiIPR001330. Prenyltrans.
    IPR018333. Squalene_cyclase.
    IPR002365. Terpene_synthase_CS.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PfamiPF00432. Prenyltrans. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 2 hits.
    TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
    PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E2IUA9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWKLKIADGG SNPYIFTTNN FVGRQIWEFD PQATDPQQLA KVEAARLDFY    50
    HNRYKLKPNS DLLWRMQFLE EKAFTQTIPQ VKVEDGEEVS YEAVTAALRR 100
    GVHLYSALQA SDGHWPAENA GPMFFMPPMV MCLYITGHLN AIFTEEHRSE 150
    TLRYIYYHQN EDGGWGFHIE GHSTMFGTVL NYICMRLLGE GPEGGQDNAV 200
    SRGRKWILDH GGATSIPSWG KTWLSIMGLC DWSGCNPMPP EFWLLPSYLP 250
    MHPGKMWCYC RMVYMPMSYL YGKRFTARIT PLILQLREEI HIQPYDQIDW 300
    KKVRHVCCKE DMYYPHPLLQ DLLWDTLYLT TEPLLTRWPL NKLIRKRALQ 350
    TTMKHIHYED ENSRYITIGC VEKVLCMLAC WVEDPNGDYF KKHLARIPDY 400
    LWIAEDGMKM QSFGSQHWDT AFSIQALLAS NMAEEIGITL AKGHDFIKKS 450
    QVKDNPSGDF KGMYRHISKG AWTFSDQDHG WQVSDCTAEG LKCCLLFSMM 500
    QPEVVGESMA PESLYNSVNV LLSLQSQNGG LPAWEPAGAP EWLELLNPTE 550
    FFENIVIEHE YVECTSSAVQ ALVLFKKLYP LHRRKEVERF ITNGAKYLED 600
    IQMPDGSWYG NWGVCFTYGA WFALEGLSAA GKTYNNCAAV RKGVDFLLNI 650
    QLEDGGWGES YQSCPDKKYV PLEDNRSNLV QTSWALMGLI YAGQADRDPT 700
    PLHRAAQLLI NSQLEDGDFP QQEITGVFQR NCMLHYAAYR NIFPLWALAE 750
    YRRQIQLHSE ATKMV 765
    Length:765
    Mass (Da):87,975
    Last modified:November 30, 2010 - v1
    Checksum:iFBA2A79C30558B7C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HM623871 mRNA. Translation: ADK35126.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HM623871 mRNA. Translation: ADK35126.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.50.10.20. 2 hits.
    InterProi IPR001330. Prenyltrans.
    IPR018333. Squalene_cyclase.
    IPR002365. Terpene_synthase_CS.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view ]
    Pfami PF00432. Prenyltrans. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 2 hits.
    TIGRFAMsi TIGR01787. squalene_cyclas. 1 hit.
    PROSITEi PS01074. TERPENE_SYNTHASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of oxidosqualene cyclases from Kalanchoe daigremontiana: enzymes catalyzing up to 10 rearrangement steps yielding friedelin and other triterpenoids."
      Wang Z., Yeats T., Han H., Jetter R.
      J. Biol. Chem. 285:29703-29712(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiLUPS_KALDA
    AccessioniPrimary (citable) accession number: E2IUA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3