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E2IUA9

- LUPS_KALDA

UniProt

E2IUA9 - LUPS_KALDA

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Protein

Lupeol synthase

Gene
N/A
Organism
Kalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Oxidosqualene cyclase that generates lupeol, a triterpenoid product. Lupeol is probably required to coat the leaf exterior as a defense compound against pathogens or herbivores.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = lupeol.1 Publication

GO - Molecular functioni

  1. intramolecular transferase activity Source: UniProtKB

GO - Biological processi

  1. triterpenoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Lupeol synthase (EC:5.4.99.41)
Short name:
KdLUS
OrganismiKalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum)
Taxonomic identifieri23013 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeSaxifragalesCrassulaceaeKalanchoe

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 765765Lupeol synthasePRO_0000418481Add
BLAST

Expressioni

Tissue specificityi

Expressed only in the epidermal cells on both sides of the leaf and not in internal leaf tissues.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati148 – 18942PFTB 1Add
BLAST
Repeati640 – 68142PFTB 2Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 2 PFTB repeats.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E2IUA9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWKLKIADGG SNPYIFTTNN FVGRQIWEFD PQATDPQQLA KVEAARLDFY
60 70 80 90 100
HNRYKLKPNS DLLWRMQFLE EKAFTQTIPQ VKVEDGEEVS YEAVTAALRR
110 120 130 140 150
GVHLYSALQA SDGHWPAENA GPMFFMPPMV MCLYITGHLN AIFTEEHRSE
160 170 180 190 200
TLRYIYYHQN EDGGWGFHIE GHSTMFGTVL NYICMRLLGE GPEGGQDNAV
210 220 230 240 250
SRGRKWILDH GGATSIPSWG KTWLSIMGLC DWSGCNPMPP EFWLLPSYLP
260 270 280 290 300
MHPGKMWCYC RMVYMPMSYL YGKRFTARIT PLILQLREEI HIQPYDQIDW
310 320 330 340 350
KKVRHVCCKE DMYYPHPLLQ DLLWDTLYLT TEPLLTRWPL NKLIRKRALQ
360 370 380 390 400
TTMKHIHYED ENSRYITIGC VEKVLCMLAC WVEDPNGDYF KKHLARIPDY
410 420 430 440 450
LWIAEDGMKM QSFGSQHWDT AFSIQALLAS NMAEEIGITL AKGHDFIKKS
460 470 480 490 500
QVKDNPSGDF KGMYRHISKG AWTFSDQDHG WQVSDCTAEG LKCCLLFSMM
510 520 530 540 550
QPEVVGESMA PESLYNSVNV LLSLQSQNGG LPAWEPAGAP EWLELLNPTE
560 570 580 590 600
FFENIVIEHE YVECTSSAVQ ALVLFKKLYP LHRRKEVERF ITNGAKYLED
610 620 630 640 650
IQMPDGSWYG NWGVCFTYGA WFALEGLSAA GKTYNNCAAV RKGVDFLLNI
660 670 680 690 700
QLEDGGWGES YQSCPDKKYV PLEDNRSNLV QTSWALMGLI YAGQADRDPT
710 720 730 740 750
PLHRAAQLLI NSQLEDGDFP QQEITGVFQR NCMLHYAAYR NIFPLWALAE
760
YRRQIQLHSE ATKMV
Length:765
Mass (Da):87,975
Last modified:November 30, 2010 - v1
Checksum:iFBA2A79C30558B7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM623871 mRNA. Translation: ADK35126.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM623871 mRNA. Translation: ADK35126.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.20. 2 hits.
InterProi IPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view ]
Pfami PF00432. Prenyltrans. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 2 hits.
TIGRFAMsi TIGR01787. squalene_cyclas. 1 hit.
PROSITEi PS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of oxidosqualene cyclases from Kalanchoe daigremontiana: enzymes catalyzing up to 10 rearrangement steps yielding friedelin and other triterpenoids."
    Wang Z., Yeats T., Han H., Jetter R.
    J. Biol. Chem. 285:29703-29712(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLUPS_KALDA
AccessioniPrimary (citable) accession number: E2IUA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: November 30, 2010
Last modified: October 1, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3