E2IUA9 (LUPS_KALDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lupeol synthase Short name=KdLUS EC=5.4.99.41
Organism	Kalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum)
Taxonomic identifier	23013 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Saxifragales › Crassulaceae › Kalanchoe

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oxidosqualene cyclase that generates lupeol, a triterpenoid product. Lupeol is probably required to coat the leaf exterior as a defense compound against pathogens or herbivores. Ref.1
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = lupeol. Ref.1
Tissue specificity	Expressed only in the epidermal cells on both sides of the leaf and not in internal leaf tissues. Ref.1
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 2 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Isomerase
Gene Ontology (GO)
Biological_process	triterpenoid biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	intramolecular transferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 765	765	Lupeol synthase		PRO_0000418481
Regions
	Repeat	148 – 189	42	PFTB 1
	Repeat	640 – 681	42	PFTB 2

Sequences

	Sequence		Length	Mass (Da)
	E2IUA9 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: FBA2A79C30558B7C	FASTA	765	87,975
	10 20 30 40 50 60 MWKLKIADGG SNPYIFTTNN FVGRQIWEFD PQATDPQQLA KVEAARLDFY HNRYKLKPNS 70 80 90 100 110 120 DLLWRMQFLE EKAFTQTIPQ VKVEDGEEVS YEAVTAALRR GVHLYSALQA SDGHWPAENA 130 140 150 160 170 180 GPMFFMPPMV MCLYITGHLN AIFTEEHRSE TLRYIYYHQN EDGGWGFHIE GHSTMFGTVL 190 200 210 220 230 240 NYICMRLLGE GPEGGQDNAV SRGRKWILDH GGATSIPSWG KTWLSIMGLC DWSGCNPMPP 250 260 270 280 290 300 EFWLLPSYLP MHPGKMWCYC RMVYMPMSYL YGKRFTARIT PLILQLREEI HIQPYDQIDW 310 320 330 340 350 360 KKVRHVCCKE DMYYPHPLLQ DLLWDTLYLT TEPLLTRWPL NKLIRKRALQ TTMKHIHYED 370 380 390 400 410 420 ENSRYITIGC VEKVLCMLAC WVEDPNGDYF KKHLARIPDY LWIAEDGMKM QSFGSQHWDT 430 440 450 460 470 480 AFSIQALLAS NMAEEIGITL AKGHDFIKKS QVKDNPSGDF KGMYRHISKG AWTFSDQDHG 490 500 510 520 530 540 WQVSDCTAEG LKCCLLFSMM QPEVVGESMA PESLYNSVNV LLSLQSQNGG LPAWEPAGAP 550 560 570 580 590 600 EWLELLNPTE FFENIVIEHE YVECTSSAVQ ALVLFKKLYP LHRRKEVERF ITNGAKYLED 610 620 630 640 650 660 IQMPDGSWYG NWGVCFTYGA WFALEGLSAA GKTYNNCAAV RKGVDFLLNI QLEDGGWGES 670 680 690 700 710 720 YQSCPDKKYV PLEDNRSNLV QTSWALMGLI YAGQADRDPT PLHRAAQLLI NSQLEDGDFP 730 740 750 760 QQEITGVFQR NCMLHYAAYR NIFPLWALAE YRRQIQLHSE ATKMV

References

[1]

"Cloning and characterization of oxidosqualene cyclases from Kalanchoe daigremontiana: enzymes catalyzing up to 10 rearrangement steps yielding friedelin and other triterpenoids." Wang Z., Yeats T., Han H., Jetter R. J. Biol. Chem. 285:29703-29712(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HM623871 mRNA. Translation: ADK35126.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUPS_KALDA

Accession

Primary (citable) accession number: E2IUA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2012
Last sequence update:	November 30, 2010
Last modified:	October 3, 2012
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program