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Protein

Friedelin synthase

Gene
N/A
Organism
Kalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Oxidosqualene cyclase that generates friedelin, a triterpenoid product with the maximum number of rearrangements possible. Friedelin is probably required to coat the leaf exterior as defense compound against pathogens or herbivores.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = friedelin.1 Publication

GO - Molecular functioni

  1. intramolecular transferase activity Source: UniProtKB

GO - Biological processi

  1. triterpenoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17975.

Names & Taxonomyi

Protein namesi
Recommended name:
Friedelin synthase (EC:5.4.99.50)
Short name:
KdFRS
OrganismiKalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum)
Taxonomic identifieri23013 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeSaxifragalesCrassulaceaeKalanchoe

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Friedelin synthasePRO_0000418483Add
BLAST

Expressioni

Tissue specificityi

Expressed only in the epidermal cells on both sides of the leaf and not in internal leaf tissues.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati148 – 18942PFTB 1Add
BLAST
Repeati640 – 68142PFTB 2Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 2 PFTB repeats.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E2IUA8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWKLKIAEGG SDPYIYTTNN FVGRQIWEFD PQATDPQQLA KVEAARLNFY
60 70 80 90 100
NHRHKIKPSS DLLWRLQFLE EKDFRQNIAQ VKVEDGEEVS YEAATAALKR
110 120 130 140 150
GVHFYSALQA SDGHWPAENA GPMFFMSPLV MCLYITGHLN TIFTEEHRRE
160 170 180 190 200
TLRYIYYHQN EDGGWGFHIE GQSTMFGTVL NYICMRLLGE GPEGGQDNAV
210 220 230 240 250
SRGRKWILDH GGATAIPSWG KTWLSIMGLC DWSGCNPMPP EFWLLPSYLP
260 270 280 290 300
MHPAKMWCYC RMVYMPMSYL YGKRFTTHIT PLILQLREEL HTQPYDQINW
310 320 330 340 350
KKVRHVCCKE DTYYPHPILQ DLIWDTLYLT TEPLLTRWPL NKLIRERALK
360 370 380 390 400
KTMKHIHYED ENSRYIVIGA VEKVLCMLAC WVEDPNGDYF KKHLARVPDY
410 420 430 440 450
FWVAEDGMKI QSFGSQHWDT AFFVQALLAS DMTDEIRTTL AKAHDCIKKS
460 470 480 490 500
QVKDNPSGDF RSMYRHISKG AWTFSDQDHG WQLSDCTAEG LKCCLLFSLM
510 520 530 540 550
QPEVVGEAMP PERLYDSVNV LLYLQSKNGG MPGWEPAGES EWLELLNPTE
560 570 580 590 600
FFENIVIEHE YVECTSSAVQ ALVLFKKLYP LHRRKEVERF ITNGAKYLED
610 620 630 640 650
IQMPDGSWYG NWGVCFTYGA WFALEGLSAA GKTYNNCAAV RKGVDFLLNI
660 670 680 690 700
QLEDGGWGES YQSCPDKKYV PLEDNRSNLV QTSWALMGLI YAGQADRDPT
710 720 730 740 750
PLHRAAKLLI NSQLEDGDFP QQEIAGVFKM NCTLHFAAYR NIFPIWALAV
760
YRRFCNPNSE AISKPSK
Length:767
Mass (Da):88,396
Last modified:November 30, 2010 - v1
Checksum:iA9F85D0CFB092B74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM623870 mRNA. Translation: ADK35125.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM623870 mRNA. Translation: ADK35125.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17975.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of oxidosqualene cyclases from Kalanchoe daigremontiana: enzymes catalyzing up to 10 rearrangement steps yielding friedelin and other triterpenoids."
    Wang Z., Yeats T., Han H., Jetter R.
    J. Biol. Chem. 285:29703-29712(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiFRIES_KALDA
AccessioniPrimary (citable) accession number: E2IUA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: November 30, 2010
Last modified: October 1, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.