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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

CHLNCDRAFT_30873

Organism
Chlorella variabilis (Green alga)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism.UniRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional dihydrofolate reductase-thymidylate synthase (CHLNCDRAFT_30873)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei390UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotation, OxidoreductaseUniRule annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotation, One-carbon metabolismUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
Gene namesi
ORF Names:CHLNCDRAFT_30873Imported
OrganismiChlorella variabilis (Green alga)Imported
Taxonomic identifieri554065 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaTrebouxiophyceaeChlorellalesChlorellaceaeChlorella
Proteomesi
  • UP000008141 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 206DHFRInterPro annotationAdd BLAST198

Sequence similaritiesi

In the C-terminal section; belongs to the thymidylate synthase family.UniRule annotation
In the N-terminal section; belongs to the dihydrofolate reductase family.UniRule annotation

Phylogenomic databases

InParanoidiE1ZCI9.
KOiK13998.
OMAiVYSNHVE.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
cd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR036926. Thymidate_synth/dCMP_Mease_sf.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PfamiView protein in Pfam
PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
PIRSFiPIRSF000389. DHFR-TS. 2 hits.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiView protein in PROSITE
PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

E1ZCI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEQARRTI QIVVAATKQW GIGKGGSLPW SLPGDMKYFR ELTSRTADPA
60 70 80 90 100
KQNVVIMGRK TWESIPAKFR PLAGRINVVL TRGAVAGDEN ASAPGNATAA
110 120 130 140 150
LAEASKAEGV HISSSLDSAL EMLSGPEFDS RVESVFVIGG GQVYKECMES
160 170 180 190 200
PLLSAIHLTL VEGEAAEAAN CDTFMPPVDE SRFRLWSASA PRSVGGTRYS
210 220 230 240 250
FLCYTRAGQE GLPALPPTVA SRHEEHQYLE MIEDVMANGV FRGDRTGTGT
260 270 280 290 300
YSKFGATMRF NLRHSFPLLT TKRVFWRGVA EELLWFVSGC TNARVLMDKG
310 320 330 340 350
IHIWDGNGSR EYLDSIGLSH REEMDLGPVY GFQWRHFGAE YTDMHADYGG
360 370 380 390 400
KGVDQLAELI HKIKTNPNDR RLVLSAWNPA ALTDMALPPC HMFCQFYVAD
410 420 430 440 450
GELSCQMYQR SCDLGLGVPF NIASYSLLTC MVAQVCGLRP GDFVHVLGDA
460 470 480 490 500
HVYANHVGPL RDQLKNAPRH FPRLLINPAK TDIDSFVFDD FQLEGYQPHK

AIKMQMAV
Length:508
Mass (Da):56,086
Last modified:November 30, 2010 - v1
Checksum:iBB4AE691DDEC4312
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL433842 Genomic DNA. Translation: EFN56442.1.
RefSeqiXP_005848544.1. XM_005848482.1.

Genome annotation databases

GeneIDi17355832.
KEGGicvr:CHLNCDRAFT_30873.

Similar proteinsi

Entry informationi

Entry nameiE1ZCI9_CHLVA
AccessioniPrimary (citable) accession number: E1ZCI9
Entry historyiIntegrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: October 25, 2017
This is version 44 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported