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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

CHLNCDRAFT_30873

Organism
Chlorella variabilis (Green alga)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism.UniRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional dihydrofolate reductase-thymidylate synthase (CHLNCDRAFT_30873)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei390UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotation, OxidoreductaseUniRule annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotation, One-carbon metabolismUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
Gene namesi
ORF Names:CHLNCDRAFT_30873Imported
OrganismiChlorella variabilis (Green alga)Imported
Taxonomic identifieri554065 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaTrebouxiophyceaeChlorellalesChlorellaceaeChlorella
Proteomesi
  • UP000008141 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 206DHFRInterPro annotationAdd BLAST198

Sequence similaritiesi

In the C-terminal section; belongs to the thymidylate synthase family.UniRule annotation
In the N-terminal section; belongs to the dihydrofolate reductase family.UniRule annotation

Phylogenomic databases

InParanoidiE1ZCI9
KOiK13998
OMAiVYSNHVE

Family and domain databases

CDDicd00209 DHFR, 1 hit
cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
3.40.430.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR012262 DHFR-TS
IPR017925 DHFR_CS
IPR001796 DHFR_dom
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PANTHERiPTHR11548:SF7 PTHR11548:SF7, 2 hits
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PF00303 Thymidylat_synt, 1 hit
PIRSFiPIRSF000389 DHFR-TS, 2 hits
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF53597 SSF53597, 1 hit
SSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

E1ZCI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEQARRTI QIVVAATKQW GIGKGGSLPW SLPGDMKYFR ELTSRTADPA
60 70 80 90 100
KQNVVIMGRK TWESIPAKFR PLAGRINVVL TRGAVAGDEN ASAPGNATAA
110 120 130 140 150
LAEASKAEGV HISSSLDSAL EMLSGPEFDS RVESVFVIGG GQVYKECMES
160 170 180 190 200
PLLSAIHLTL VEGEAAEAAN CDTFMPPVDE SRFRLWSASA PRSVGGTRYS
210 220 230 240 250
FLCYTRAGQE GLPALPPTVA SRHEEHQYLE MIEDVMANGV FRGDRTGTGT
260 270 280 290 300
YSKFGATMRF NLRHSFPLLT TKRVFWRGVA EELLWFVSGC TNARVLMDKG
310 320 330 340 350
IHIWDGNGSR EYLDSIGLSH REEMDLGPVY GFQWRHFGAE YTDMHADYGG
360 370 380 390 400
KGVDQLAELI HKIKTNPNDR RLVLSAWNPA ALTDMALPPC HMFCQFYVAD
410 420 430 440 450
GELSCQMYQR SCDLGLGVPF NIASYSLLTC MVAQVCGLRP GDFVHVLGDA
460 470 480 490 500
HVYANHVGPL RDQLKNAPRH FPRLLINPAK TDIDSFVFDD FQLEGYQPHK

AIKMQMAV
Length:508
Mass (Da):56,086
Last modified:November 30, 2010 - v1
Checksum:iBB4AE691DDEC4312
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL433842 Genomic DNA Translation: EFN56442.1
RefSeqiXP_005848544.1, XM_005848482.1

Genome annotation databases

GeneIDi17355832
KEGGicvr:CHLNCDRAFT_30873

Similar proteinsi

Entry informationi

Entry nameiE1ZCI9_CHLVA
AccessioniPrimary (citable) accession number: E1ZCI9
Entry historyiIntegrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: February 28, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health