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Protein

Pectate lyase

Gene

amba2.01

Organism
Ambrosia artemisiifolia (Short ragweed)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.UniRule annotation

Cofactori

Ca2+UniRule annotationNote: Binds 1 Ca2+ ion. Required for its activity.UniRule annotation

Pathway:ipectin degradation

This protein is involved in step 2 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Pectate lyase 4, Pectate lyase 1, Pectate lyase 2, Pectate lyase (amba1.3), Pectate lyase (amba1.3), Pectate lyase (amba1.2), Pectate lyase (amba1.3), Pectate lyase (amba1.4), Pectate lyase (amba1.3), Pectate lyase (amba1.3), Pectate lyase (amba1), Pectate lyase (amba2.01), Pectate lyase (amba2.01), Pectate lyase 5, Pectate lyase 3
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported

Keywords - Ligandi

CalciumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyaseUniRule annotation (EC:4.2.2.2UniRule annotation)
Gene namesi
Name:amba2.01Imported
OrganismiAmbrosia artemisiifolia (Short ragweed)Imported
Taxonomic identifieri4212 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Pathology & Biotechi

Protein family/group databases

Allergomei24. Amb a 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 PotentialImportedAdd
BLAST
Signal peptidei1 – 2222UniRule annotationAdd
BLAST
Chaini23 – 397375Pectate lyaseUniRule annotationPRO_5001341879Add
BLAST
Chaini26 – 397372 PotentialImportedPRO_5000643453Add
BLAST
Chaini26 – 397372pollen allergen Amb a 2.01ImportedPRO_5000643454Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide lyase 1 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotationImported

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
SM00710. PbH1. 3 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E1XUM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIKHCCYIL YFTLALVTLV QAGRLGEEVD ILPSPNDTRR SLQGCEAHNI
60 70 80 90 100
IDKCWRCKPD WAENRQALGD CAQGFGKATH GGKWGDIYMV TSDQDDDVVN
110 120 130 140 150
PKEGTLRFGA TQDRPLWIIF QRDMIIYLQQ EMVVTSDKTI DGRGAKVELV
160 170 180 190 200
YGGITLMNVK NVIIHNIDIH DVRVLPGGRI KSNGGPAIPR HQSDGDAIHV
210 220 230 240 250
TGSSDIWIDH CTLSKSFDGL VDVNWGSTGV TISNCKFTHH EKAVLLGASD
260 270 280 290 300
THFQDLKMHV TLAYNIFTNT VHERMPRCRF GFFQIVNNFY DRWDKYAIGG
310 320 330 340 350
SSNPTILSQG NKFVAPDFIY KKNVCLRTGA QEPEWMTWNW RTQNDVLENG
360 370 380 390
AIFVASGSDP VLTAEQNAGM MQAEPGDMVP QLTMNAGVLT CSPGAPC
Length:397
Mass (Da):44,083
Last modified:November 30, 2010 - v1
Checksum:iF0E4BFE1ABA6B414
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR669665 mRNA. Translation: CBW30994.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR669665 mRNA. Translation: CBW30994.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei24. Amb a 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
SM00710. PbH1. 3 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Isoform analysis of Amb a 1 and Amb a 2."
    Nandy A., Mitulski L.
    Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiE1XUM0_AMBAR
AccessioniPrimary (citable) accession number: E1XUM0
Entry historyi
Integrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: February 4, 2015
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.