ID   E1XUL4_AMBAR            Unreviewed;       397 AA.
AC   E1XUL4;
DT   30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT   30-NOV-2010, sequence version 1.
DT   27-MAR-2024, entry version 41.
DE   RecName: Full=Pectate lyase {ECO:0000256|ARBA:ARBA00012272, ECO:0000256|RuleBase:RU361123};
DE            EC=4.2.2.2 {ECO:0000256|ARBA:ARBA00012272, ECO:0000256|RuleBase:RU361123};
GN   Name=amba1.3 {ECO:0000313|EMBL:CBW30988.1};
OS   Ambrosia artemisiifolia (Common ragweed).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC   Heliantheae alliance; Heliantheae; Ambrosia.
OX   NCBI_TaxID=4212 {ECO:0000313|EMBL:CBW30988.1};
RN   [1] {ECO:0000313|EMBL:CBW30988.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Nandy A., Mitulski L.;
RT   "Isoform analysis of Amb a 1 and Amb a 2.";
RL   Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Has pectate lyase activity. {ECO:0000256|ARBA:ARBA00002799}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC         oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC         their non-reducing ends.; EC=4.2.2.2;
CC         Evidence={ECO:0000256|ARBA:ARBA00000695,
CC         ECO:0000256|RuleBase:RU361123};
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|RuleBase:RU361123};
CC       Note=Binds 1 Ca(2+) ion. Required for its activity.
CC       {ECO:0000256|RuleBase:RU361123};
CC   -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-
CC       gluconate from pectin: step 2/5. {ECO:0000256|ARBA:ARBA00005220,
CC       ECO:0000256|RuleBase:RU361123}.
CC   -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family. Amb a
CC       subfamily. {ECO:0000256|ARBA:ARBA00008800}.
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DR   EMBL; FR669659; CBW30988.1; -; mRNA.
DR   AlphaFoldDB; E1XUL4; -.
DR   Allergome; 24; Amb a 1.
DR   Allergome; 9519; Amb a 1.0304.
DR   UniPathway; UPA00545; UER00824.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030570; F:pectate lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.160.20.10; Single-stranded right-handed beta-helix, Pectin lyase-like; 1.
DR   InterPro; IPR018082; AmbAllergen.
DR   InterPro; IPR002022; Pec_lyase.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   InterPro; IPR045032; PEL.
DR   PANTHER; PTHR31683:SF159; PECTATE LYASE; 1.
DR   PANTHER; PTHR31683; PECTATE LYASE 18-RELATED; 1.
DR   Pfam; PF00544; Pectate_lyase_4; 1.
DR   PRINTS; PR00807; AMBALLERGEN.
DR   SMART; SM00656; Amb_all; 1.
DR   SUPFAM; SSF51126; Pectin lyase-like; 1.
PE   2: Evidence at transcript level;
KW   Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|RuleBase:RU361123};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|RuleBase:RU361123};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|RuleBase:RU361123}; Signal {ECO:0000256|RuleBase:RU361123}.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000256|RuleBase:RU361123"
FT   CHAIN           26..397
FT                   /note="Pectate lyase"
FT                   /evidence="ECO:0000256|RuleBase:RU361123"
FT                   /id="PRO_5005127633"
FT   DOMAIN          123..319
FT                   /note="Pectate lyase"
FT                   /evidence="ECO:0000259|SMART:SM00656"
SQ   SEQUENCE   397 AA;  42913 MW;  C079412575853D0A CRC64;
     MGIKQCCYIL YFTLALVALL QPVRSAEGVG EILPSVNETR SLQACEALNI IDKCWRGKAD
     WENNRQALAD CAQGFAKGTY GGKWGDVYTV TSNLDDDVAN PKEGTLRFAA AQNRPLWIIF
     KNDMVINLNQ ELVVNSDKTI DGRGVKVEII NGGLTLMNVK NIIIHNINIH DVKVLPGGMI
     KSNDGPPILR QASDGDTINV AGSSQIWIDH CSLSKSFDGL VDVTLGSTHV TISNCKFTQQ
     SKAILLGADD THVQDKGMLA TVAFNMFTDN VDQRMPRCRF GFFQVVNNNY DRWGTYAIGG
     SSAPTILCQG NRFLAPDDQI KKNVLARTGT GAAESMAWNW RSDKDLLENG AIFVTSGSDP
     VLTPVQSAGM IPAEPGEAAI KLTSSAGVLS CRPGAPC
//