Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

E1X227 (E1X227_BACMS) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL CBW26687.1
Ordered Locus Names:BMS_1867 EMBL CBW26687.1
OrganismBacteriovorax marinus (strain ATCC BAA-682 / DSM 15412 / SJ) [Complete proteome] [HAMAP] EMBL CBW26687.1
Taxonomic identifier862908 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaBdellovibrionalesBacteriovoracaceaeBacteriovorax

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain121 – 326206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding151 – 20656ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2801Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2931Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2931Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2951Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E1X227 [UniParc].

Last modified November 30, 2010. Version 1.
Checksum: 93F4D612E4161D96

FASTA32937,030
        10         20         30         40         50         60 
MKNILLLCGG GGSEHEVSLR SANYIYDELE KVNLYNVFKV EIKANGDRVD HHGQLCELRK 

        70         80         90        100        110        120 
AGELYYPSSD QTHKLDFAIP CIHGPPGENG VIQSIFELMG LPYLGCSPEP SQTCFNKVST 

       130        140        150        160        170        180 
KLWLDALKVP NTPYIYLSDE SRESISAAEE FFNANKDVYI KAASQGSSVG CYHVTSLEDL 

       190        200        210        220        230        240 
ESTILSAFKF SEYVLIERTL KARELEVSAF EYNNEVVITA PGEIICPDGF YDYDQKYSND 

       250        260        270        280        290        300 
SATKTEVNAK GLSAEQINDI KSYSLKAFKG LKLRHLSRID FFLTEEGKVY LNEINTFPGM 

       310        320 
TSISMFPKMM ENHGVKFSEY LRKIIEEST 

« Hide

References

[1]"A small predatory core genome in the divergent marine Bacteriovorax marinus SJ and the terrestrial Bdellovibrio bacteriovorus."
Crossman L.C., Chen H., Cerdeno-Tarraga A.M., Brooks K., Quail M.A., Pineiro S.A., Hobley L., Sockett R.E., Bentley S.D., Parkhill J., Williams H.N., Stine O.C.
ISME J. 7:148-160(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-682 / DSM 15412 / SJ.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FQ312005 Genomic DNA. Translation: CBW26687.1.
RefSeqYP_005035652.1. NC_016620.1.

3D structure databases

ProteinModelPortalE1X227.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBW26687; CBW26687; BMS_1867.
GeneID11549316.
KEGGbmx:BMS_1867.
PATRIC42739739. VBIBacMar168520_1772.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.
OMAHNGLLKN.

Enzyme and pathway databases

BioCycBMAR862908:GJBX-1765-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE1X227_BACMS
AccessionPrimary (citable) accession number: E1X227
Entry history
Integrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: June 11, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)