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E1WGG8 (OTSA_SALTS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

EC=2.4.1.15
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene names
Name:otsA
Ordered Locus Names:SL1344_1862
OrganismSalmonella typhimurium (strain SL1344) [Complete proteome] [HAMAP]
Taxonomic identifier216597 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtrehalose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionalpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 473472Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
PRO_0000405417

Regions

Region21 – 222UDP-glucose binding By similarity
Region365 – 3695UDP-glucose binding By similarity

Sites

Binding site101Glucose-6-phosphate By similarity
Binding site761Glucose-6-phosphate By similarity
Binding site1301Glucose-6-phosphate By similarity
Binding site2621UDP-glucose By similarity
Binding site2671UDP-glucose By similarity
Binding site3001Glucose-6-phosphate By similarity
Binding site3391UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity
Site851Involved in alpha anomer selectivity By similarity
Site1551Involved in alpha anomer selectivity By similarity

Sequences

Sequence LengthMass (Da)Tools
E1WGG8 [UniParc].

Last modified November 30, 2010. Version 1.
Checksum: E85B3F0AE58E144A

FASTA47353,581
        10         20         30         40         50         60 
MSRLVVVSNR IAPPDNKGGA GGLAVGVLGA LKAAGGLWFG WSGETGNEDE PLKKVTKGNI 

        70         80         90        100        110        120 
TWASFNLSEQ DYEDYYCQFS NAVLWPAFHY RLDLVQFQRP AWEGYMRVNA LLADKLLPLI 

       130        140        150        160        170        180 
KENDIIWVHD YHLLPFASEL RKRGVNNRIG FFLHIPFPTP EIFNALPPHD ELLEQLCDFD 

       190        200        210        220        230        240 
LLGFQTENDR LAFLDSLSSQ TRVTTRSGKQ HIAWGKDFQT EVYPIGIEPD EIALQAAGPL 

       250        260        270        280        290        300 
PPKLAQLKAE LKNVKNIFSV ERLDYSKGLP ERFLAYEALL ENYPQHRGKI RYTQIAPTSR 

       310        320        330        340        350        360 
GEVQAYQDIR HQLETEAGRI NGKYGQLGWT PLYYLNQHFD RKLLMKIFRY SDVGLVTPLR 

       370        380        390        400        410        420 
DGMNLVAKEF VAAQDPANPG VLVLSQFAGA ANELTSALIV NPYDRDDVAA ALNRALTMPL 

       430        440        450        460        470 
AERISRHAEM LDVIVKNDIN RWQERFIHDL KEVTPRSPER QQQNNVATFP KLA 

« Hide

References

« Hide 'large scale' references
[1]"Trehalose biosynthesis operon in S. typhimurium."
Howells A.M., Bullifent H.L., Dhaliwal K., Griffin K., Frith G., Tunnacliffe A., Titball R.W.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SL1344.
[2]"The transcriptional landscape and small RNAs of Salmonella enterica serovar Typhimurium."
Kroger C., Dillon S.C., Cameron A.D., Papenfort K., Sivasankaran S.K., Hokamp K., Chao Y., Sittka A., Hebrard M., Handler K., Colgan A., Leekitcharoenphon P., Langridge G.C., Lohan A.J., Loftus B., Lucchini S., Ussery D.W., Dorman C.J. expand/collapse author list , Thomson N.R., Vogel J., Hinton J.C.
Proc. Natl. Acad. Sci. U.S.A. 109:E1277-E1286(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SL1344.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF213176 Genomic DNA. Translation: AAF34582.1.
FQ312003 Genomic DNA. Translation: CBW17956.1.
RefSeqYP_005181756.1. NC_016810.1.

3D structure databases

ProteinModelPortalE1WGG8.
SMRE1WGG8. Positions 2-456.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEE1WGG8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBW17956; CBW17956; SL1344_1862.
PATRIC43189451. VBISalEnt88447_2067.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAWGESFVK.

Enzyme and pathway databases

BioCycSENT216597:GJB7-1885-MONOMER.
UniPathwayUPA00299.

Family and domain databases

InterProIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameOTSA_SALTS
AccessionPrimary (citable) accession number: E1WGG8
Secondary accession number(s): P0A1Q0, Q9L893
Entry history
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 30, 2010
Last modified: May 14, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways