E1WGF1 (PBP2_SALTS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Penicillin-binding protein 2
Short name=PBP-2

Gene names

Name:	mrdA
Synonyms:	pbpA
Ordered Locus Names:	SL1344_1845

Organism

Salmonella typhimurium (strain SL1344) [Complete proteome] [HAMAP]

Taxonomic identifier

216597 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross-linked peptidoglycan from the lipid intermediates By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Domain	Has a penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits) By similarity.
Sequence similarities	Belongs to the transpeptidase family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	catalytic activity Inferred from electronic annotation. Source: UniProtKB-KW penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	Penicillin-binding protein 2		PRO_0000405425
Sites
	Active site	326	1	Acyl-ester intermediate By similarity
Experimental info
	Sequence conflict	62	1	P → A in AAC44611. Ref.2
	Sequence conflict	103	1	P → R in AAC44611. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	E1WGF1 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: F59E950C293A5E71	FASTA	623	69,833
	10 20 30 40 50 60 MTFKDFDAEE KLFLRRVIVA FGVVVVCFGI LIFNLYNLQI RQHHYYTTRS NENDIKMLPV 70 80 90 100 110 120 APTRGIIYDR NGIPLVRNVT WYDIAVTPYK IADMDALLKQ LTPIVDLSPD DISDFRRALK 130 140 150 160 170 180 SSSRYRPVVL KNALTDVEIA RFAVNQFHFN GVTINSYQDR QYPYGAELAH VLGYVSKIND 190 200 210 220 230 240 NDLKALDKKG LAENYAADHN IGKQGIERYY ENDLHGKTGY QEVEVDNHGR IVRLLKDVPP 250 260 270 280 290 300 IAGKNIHLTL DLHLQEYIES LLAGQRAAVL VEDPHDGSVL AMVSMPSYDP NPFVKGISYQ 310 320 330 340 350 360 DYGKLLHDKN LPLINRVTQG LYPPASTVKP YMAMSALLCG IITPQTTFFG APTWTLPGTQ 370 380 390 400 410 420 RHYRDWKKTG HGMLDVTKAI EESADTFFYQ VAYMMGIDRI DTMLSQFGYG KPTGIDLNEE 430 440 450 460 470 480 YDGLLPSRAW KQRVHKKAWY QGDTISVGIG QGYWIATPIQ MVKAMVALIN NGKVIAPHLL 490 500 510 520 530 540 LNEESGKTVV PYRPSGTPAQ IADPASPYWG LVRQAMYGMA NAPNGTGYKF FHTAPYGIAA 550 560 570 580 590 600 KSGTSQVFSL KENQTYNAKM IPIRLRDHVF YTAFAPYKNP KVAIALILEN GGSDGVTAAP 610 620 IMRKILDHLF DPQADTTQPD QAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Salmonella enterica subsp. enterica serovar Typhimurium SL1344." Dougan G., Barrow P., Achtman M., Parkhill J., Aslett M., Thomson N.R. Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL1344.
[2]	"Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction." Valdivia R.H., Falkow S. Mol. Microbiol. 22:367-378(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-106. Strain: SL1344.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FQ312003 Genomic DNA. Translation: CBW17939.1. U62714 Genomic DNA. Translation: AAC44611.1.
RefSeq	YP_005181739.1. NC_016810.1.
3D structure databases
ProteinModelPortal	E1WGF1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBW17939; CBW17939; SL1344_1845.
GeneID	11765161.
KEGG	sey:SL1344_1845.
PATRIC	43189413. VBISalEnt88447_2048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K05515.
OMA	EMLFIRR.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR005311. PBP_dimer. IPR001460. PCN-bd_Tpept. IPR017790. Penicillin-binding_protein_2. [Graphical view]
Pfam	PF03717. PBP_dimer. 1 hit. PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56519. PBP_dimer. 1 hit. SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03423. pbp2_mrdA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PBP2_SALTS

Accession

Primary (citable) accession number: E1WGF1
Secondary accession number(s): P74872

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 8, 2011
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program