E1WGF0 (SYR_SALTS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	SL1344_1844

Organism

Salmonella typhimurium (strain SL1344) [Complete proteome] [HAMAP]

Taxonomic identifier

216597 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00123
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: UniProtKB-HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_0000406087
Regions
	Motif	122 – 132	11	"HIGH" region HAMAP-Rule MF_00123
Experimental info
	Sequence conflict	491	1	D → H in AAC44610. Ref.2
	Sequence conflict	498	1	A → D in AAC44610. Ref.2
	Sequence conflict	555	1	A → V in AAC44610. Ref.2
	Sequence conflict	569	1	L → S in AAC44610. Ref.2
	Sequence conflict	574	1	V → A in AAC44610. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	E1WGF0 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: 842D46DD38EB44B7	FASTA	577	64,275
	10 20 30 40 50 60 MNIQALLSEK VSQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK LGMAPRQLAE 70 80 90 100 110 120 QVLTHLDLSG IASKVEIAGP GFINIFLEPA FLAEQVQQAL ASDRLGVSQP TRQTIVVDYS 130 140 150 160 170 180 APNVAKEMHV GHLRSTIIGD AAVRTLEFLG HHVIRANHVG DWGTQFGMLI AWLEKQQQEN 190 200 210 220 230 240 AGDMALADLE GFYRDAKKHY DEDEAFAERA RNYVVKLQSG DTYFREMWRK LVDITMTQNQ 250 260 270 280 290 300 ITYDRLNVTL TRDDVMGESL YNPMLPGIVA DLKAKGLAVE SEGATVVFLD EFKNKEGDPM 310 320 330 340 350 360 GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWT IVRKAGYVPD 370 380 390 400 410 420 SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER ARRLVAEKNP DMPADELEKL 430 440 450 460 470 480 ANAVGIGAVK YADLSKNRTT DYIFDWDNML AFEGNTAPYM QYAYTRVLSV FRKANIDEQA 490 500 510 520 530 540 LASAPVIISE DREAQLAARL LQFEETLTVV AREGTPHVMC AYLYDVAGLF SGFYEHCPIL 550 560 570 SAENDAVRNS RLKLAQLTAK TLKLGLDTLG IETVERM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape and small RNAs of Salmonella enterica serovar Typhimurium." Kroger C., Dillon S.C., Cameron A.D., Papenfort K., Sivasankaran S.K., Hokamp K., Chao Y., Sittka A., Hebrard M., Handler K., Colgan A., Leekitcharoenphon P., Langridge G.C., Lohan A.J., Loftus B., Lucchini S., Ussery D.W., Dorman C.J. , Thomson N.R., Vogel J., Hinton J.C. Proc. Natl. Acad. Sci. U.S.A. 109:E1277-E1286(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL1344.
[2]	"Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction." Valdivia R.H., Falkow S. Mol. Microbiol. 22:367-378(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 491-577. Strain: SL1344.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FQ312003 Genomic DNA. Translation: CBW17938.1. U62714 Genomic DNA. Translation: AAC44610.1.
RefSeq	YP_005181738.1. NC_016810.1.
3D structure databases
ProteinModelPortal	E1WGF0.
ModBase	Search...
MobiDB	Search...
Proteomic databases
PRIDE	E1WGF0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBW17938; CBW17938; SL1344_1844.
PATRIC	43189411. VBISalEnt88447_2047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NPNGPLH.
Enzyme and pathway databases
BioCyc	SENT216597:GJB7-1867-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF47323. SSF47323. 1 hit. SSF55190. SSF55190. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_SALTS

Accession

Primary (citable) accession number: E1WGF0
Secondary accession number(s): P74871

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 8, 2011
Last sequence update:	November 30, 2010
Last modified:	February 19, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program