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E1W9M1

- END8_SALTS

UniProt

E1W9M1 - END8_SALTS

Protein

Endonuclease 8

Gene

nei

Organism
Salmonella typhimurium (strain SL1344)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (30 Nov 2010)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotation

    Catalytic activityi

    Removes damaged bases from DNA, leaving an abasic site.UniRule annotation
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNAUniRule annotation
    Active sitei3 – 31Proton donorUniRule annotation
    Active sitei53 – 531Proton donor; for beta-elimination activityUniRule annotation
    Binding sitei70 – 701DNAUniRule annotation
    Binding sitei125 – 1251DNAUniRule annotation
    Binding sitei169 – 1691DNAUniRule annotation
    Active sitei253 – 2531Proton donor; for delta-elimination activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSENT216597:GJB7-723-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease 8UniRule annotation
    Alternative name(s):
    DNA glycosylase/AP lyase NeiUniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
    DNA-(apurinic or apyrimidinic site) lyase NeiUniRule annotation
    Endonuclease VIIIUniRule annotation
    Gene namesi
    Name:neiUniRule annotation
    Ordered Locus Names:SL1344_0710
    OrganismiSalmonella typhimurium (strain SL1344)
    Taxonomic identifieri216597 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000008962: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 263262Endonuclease 8PRO_0000405416Add
    BLAST

    Proteomic databases

    PRIDEiE1W9M1.

    Structurei

    3D structure databases

    ProteinModelPortaliE1W9M1.
    SMRiE1W9M1. Positions 2-262.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FPG family.UniRule annotation
    Contains 1 FPG-type zinc finger.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    OMAiGPDVLDM.

    Family and domain databases

    HAMAPiMF_01253. Endonuclease_8.
    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR023713. Endonuclease-VIII.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    E1W9M1-1 [UniParc]FASTAAdd to Basket

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    MPEGPEIRRA ADNLEAAIKG KPLTDVWFAF AQLKPYESQL TGQLVTRIET    50
    RGKALLTHFS NGLTLYSHNQ LYGVWRVIDT GEIPQTTRIL RVRLQTADKT 100
    ILLYSASDIE MLTAEQLTTH PFLQRVGPDV LDARLTPEEV KARLLSPRFR 150
    NRQFSGLLLD QSFLAGLGNY LRVEILWQVG LTGQHKAKDL NEAQLNALSH 200
    ALLDIPRLSY TTRGQADENK HHGALFRFKL FHRDGEACER CGGIIEKTTL 250
    SSRPFYWCPH CQK 263
    Length:263
    Mass (Da):29,863
    Last modified:November 30, 2010 - v1
    Checksum:i0BA96E876F2F19FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FQ312003 Genomic DNA. Translation: CBW16808.1.
    AF300989 Genomic DNA. Translation: AAG28771.1.
    RefSeqiYP_005180628.1. NC_016810.1.

    Genome annotation databases

    EnsemblBacteriaiCBW16808; CBW16808; SL1344_0710.
    PATRICi43186888. VBISalEnt88447_0790.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FQ312003 Genomic DNA. Translation: CBW16808.1 .
    AF300989 Genomic DNA. Translation: AAG28771.1 .
    RefSeqi YP_005180628.1. NC_016810.1.

    3D structure databases

    ProteinModelPortali E1W9M1.
    SMRi E1W9M1. Positions 2-262.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi E1W9M1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBW16808 ; CBW16808 ; SL1344_0710 .
    PATRICi 43186888. VBISalEnt88447_0790.

    Phylogenomic databases

    OMAi GPDVLDM.

    Enzyme and pathway databases

    BioCyci SENT216597:GJB7-723-MONOMER.

    Family and domain databases

    HAMAPi MF_01253. Endonuclease_8.
    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR023713. Endonuclease-VIII.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SL1344.
    2. "The redundant DNA base excision repair system of Salmonella typhimurium is conditionally required for intramacrophage survival and serves as virulence attribute."
      Suvarnapunya A.E., Guy R.L., Stein M.A.
      Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-256.
      Strain: SL1344.

    Entry informationi

    Entry nameiEND8_SALTS
    AccessioniPrimary (citable) accession number: E1W9M1
    Secondary accession number(s): Q8ZQU6, Q9F612
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 8, 2011
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3