E1W9E9 (E1W9E9_SALTS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase HAMAP-Rule MF_00049
EC=6.1.1.4 HAMAP-Rule MF_00049

Alternative name(s):
Leucyl-tRNA synthetase HAMAP-Rule MF_00049

Gene names

Name:	leuS HAMAP-Rule MF_00049 EMBL CBW16736.1
Ordered Locus Names:	SL1344_0636 EMBL CBW16736.1

Organism

Salmonella typhimurium (strain SL1344) [Complete proteome] [HAMAP] EMBL CBW16736.1

Taxonomic identifier

216597 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	860 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00049 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00049 RuleBase RU003489
Cellular component	Cytoplasm HAMAP-Rule MF_00049
Ligand	ATP-binding HAMAP-Rule MF_00049 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00049 RuleBase RU003489 EMBL CBW16736.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	42 – 52	11	"HIGH" region By similarity HAMAP-Rule MF_00049
	Motif	619 – 623	5	"KMSKS" region By similarity HAMAP-Rule MF_00049
Sites
	Binding site	622	1	ATP By similarity HAMAP-Rule MF_00049

Sequences

	Sequence		Length	Mass (Da)
	E1W9E9 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: D5003584DFBCCAB6	FASTA	860	96,985
	10 20 30 40 50 60 MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM GHVRNYTIGD 70 80 90 100 110 120 VVARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYDNIAYMKN QLKTLGFGYD 130 140 150 160 170 180 WSREIATCTP EYYRWEQKFF TELYKKGLVY KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD 190 200 210 220 230 240 TKVERKEIPQ WFIKITAYAD ELLRDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFDVKGYD 250 260 270 280 290 300 NTLTVYTTRP DTFMGATYLA VAAGHPLAQK AAANNAELAA FIDECRNTKV AEAEMATMEK 310 320 330 340 350 360 KGVDTGYKAI HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS KYGLTIKPVI 370 380 390 400 410 420 LAADGSEPDL SEQALTEKGV LFNSGEFDGL AFEAAFNAIA DKLAEKGVGE RKVNYRLRDW 430 440 450 460 470 480 GVSRQRYWGA PIPMVTLEDG TVLPTPEDQL PVILPEDVVM DGITSPIKAD PEWAKTTVNG 490 500 510 520 530 540 MPALRETDTF DTFMESSWYY ARYTCPQYQE GMLDSKAANY WLPVDIYIGG IEHAIMHLLY 550 560 570 580 590 600 FRFFHKLMRD AGMVTSDEPA KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR 610 620 630 640 650 660 IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS PADMTLEWQE 670 680 690 700 710 720 SGVEGANRFI KRVWKLVYEH TAKGPVAALN VDALSEDQKA LRRDVHKTIA KVTDDIGRRQ 730 740 750 760 770 780 TFNTAIAAIM ELMNKLAKAP QEGEQDRALL QEALQAVVRM LNPFTPHVCF TLWQELGGEG 790 800 810 820 830 840 DIDNAPWPVA DEQAMVENTT LVVVQVNGKV RGKITVPVDA TEEQVRERAG QEHLVAKYLD 850 860 GVTVRKVIYV PGKLLNLVVG

References

[1]	"The genome sequence of Salmonella enterica subsp. enterica serovar Typhimurium SL1344." Dougan G., Barrow P., Achtman M., Parkhill J., Thomson N.R. Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL1344.
[2]	"The genome sequence of Salmonella enterica subsp. enterica serovar Typhimurium SL1344." Dougan G., Barrow P., Achtman M., Parkhill J., Aslett M., Thomson N.R. Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL1344.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FQ312003 Genomic DNA. Translation: CBW16736.1.
RefSeq	YP_005180558.1. NC_016810.1.
3D structure databases
ProteinModelPortal	E1W9E9.
SMR	E1W9E9. Positions 228-413.
ModBase	Search...
Proteomic databases
PRIDE	E1W9E9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBW16736; CBW16736; SL1344_0636.
GeneID	11764239.
KEGG	sey:SL1344_0636.
PATRIC	43186711. VBISalEnt88447_0709.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01869.
OMA	GWPEQVK.
Family and domain databases
Gene3D	3.40.50.620. 3 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 3 hits. PF13603. tRNA-synt_1_2. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E1W9E9_SALTS

Accession

Primary (citable) accession number: E1W9E9

Entry history

Integrated into UniProtKB/TrEMBL:	November 30, 2010
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)