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E1W818

- AMPD_SALTS

UniProt

E1W818 - AMPD_SALTS

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Protein

1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD

Gene

ampD

Organism
Salmonella typhimurium (strain SL1344)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha-amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety (By similarity).By similarity

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+By similarityNote: Zn(2+) is required for amidase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Zinc; catalyticBy similarity
Active sitei116 – 1161Proton acceptorBy similarity
Metal bindingi154 – 1541Zinc; catalyticBy similarity
Sitei162 – 1621Transition state stabilizerBy similarity
Metal bindingi164 – 1641Zinc; catalyticBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSENT216597:GJB7-148-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (EC:3.5.1.28)
Alternative name(s):
N-acetylmuramoyl-L-alanine amidase
Gene namesi
Name:ampD
Ordered Locus Names:SL1344_0146
OrganismiSalmonella typhimurium (strain SL1344)
Taxonomic identifieri216597 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000008962: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1871871,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpDPRO_0000405414Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliE1W818.
SMRiE1W818. Positions 1-187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

OMAiCEDRAWH.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

E1W818-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLPDKGWLVE ARRVPSPHYD CRPDDEKPSL LVVHNISLPP GEFGGPWIDA
60 70 80 90 100
LFTGTIDPDA HPFFAEIAHL RVSAHCLIRR DGEIVQYVPF DKRAWHAGVS
110 120 130 140 150
NYQGRERCND FSIGIELEGT DTLAYTDAQY QQLAAVTRTL IASYPAIADN
160 170 180
MTGHCNIAPD RKTDPGPAFD WPRFRALVAL SSHKEMT
Length:187
Mass (Da):20,913
Last modified:November 30, 2010 - v1
Checksum:i9E7EBAC553AEDFE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242516 Genomic DNA. Translation: CAB89835.1.
FQ312003 Genomic DNA. Translation: CBW16249.1.
RefSeqiYP_005180083.1. NC_016810.1.

Genome annotation databases

EnsemblBacteriaiCBW16249; CBW16249; SL1344_0146.
PATRICi43185606. VBISalEnt88447_0162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242516 Genomic DNA. Translation: CAB89835.1 .
FQ312003 Genomic DNA. Translation: CBW16249.1 .
RefSeqi YP_005180083.1. NC_016810.1.

3D structure databases

ProteinModelPortali E1W818.
SMRi E1W818. Positions 1-187.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CBW16249 ; CBW16249 ; SL1344_0146 .
PATRICi 43185606. VBISalEnt88447_0162.

Phylogenomic databases

OMAi CEDRAWH.

Enzyme and pathway databases

BioCyci SENT216597:GJB7-148-MONOMER.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
[Graphical view ]
Pfami PF01510. Amidase_2. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a Salmonella-specific region located between ampE and aroP genes."
    Cano D., Casadesus J., Garcia-del Portillo F.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SL1344.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SL1344.

Entry informationi

Entry nameiAMPD_SALTS
AccessioniPrimary (citable) accession number: E1W818
Secondary accession number(s): P30013, Q9L4I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 30, 2010
Last modified: November 26, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3