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E1W818

- AMPD_SALTS

UniProt

E1W818 - AMPD_SALTS

Protein

1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD

Gene

ampD

Organism
Salmonella typhimurium (strain SL1344)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha-amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety By similarity.By similarity

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    Cofactori

    Zinc; required for amidase activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi34 – 341Zinc; catalyticBy similarity
    Active sitei116 – 1161Proton acceptorBy similarity
    Metal bindingi154 – 1541Zinc; catalyticBy similarity
    Sitei162 – 1621Transition state stabilizerBy similarity
    Metal bindingi164 – 1641Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSENT216597:GJB7-148-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (EC:3.5.1.28)
    Alternative name(s):
    N-acetylmuramoyl-L-alanine amidase
    Gene namesi
    Name:ampD
    Ordered Locus Names:SL1344_0146
    OrganismiSalmonella typhimurium (strain SL1344)
    Taxonomic identifieri216597 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000008962: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1871871,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpDPRO_0000405414Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliE1W818.
    SMRiE1W818. Positions 1-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    OMAiCEDRAWH.

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    [Graphical view]
    PfamiPF01510. Amidase_2. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    E1W818-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPDKGWLVE ARRVPSPHYD CRPDDEKPSL LVVHNISLPP GEFGGPWIDA    50
    LFTGTIDPDA HPFFAEIAHL RVSAHCLIRR DGEIVQYVPF DKRAWHAGVS 100
    NYQGRERCND FSIGIELEGT DTLAYTDAQY QQLAAVTRTL IASYPAIADN 150
    MTGHCNIAPD RKTDPGPAFD WPRFRALVAL SSHKEMT 187
    Length:187
    Mass (Da):20,913
    Last modified:November 30, 2010 - v1
    Checksum:i9E7EBAC553AEDFE1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ242516 Genomic DNA. Translation: CAB89835.1.
    FQ312003 Genomic DNA. Translation: CBW16249.1.
    RefSeqiYP_005180083.1. NC_016810.1.

    Genome annotation databases

    EnsemblBacteriaiCBW16249; CBW16249; SL1344_0146.
    PATRICi43185606. VBISalEnt88447_0162.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ242516 Genomic DNA. Translation: CAB89835.1 .
    FQ312003 Genomic DNA. Translation: CBW16249.1 .
    RefSeqi YP_005180083.1. NC_016810.1.

    3D structure databases

    ProteinModelPortali E1W818.
    SMRi E1W818. Positions 1-187.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBW16249 ; CBW16249 ; SL1344_0146 .
    PATRICi 43185606. VBISalEnt88447_0162.

    Phylogenomic databases

    OMAi CEDRAWH.

    Enzyme and pathway databases

    BioCyci SENT216597:GJB7-148-MONOMER.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    [Graphical view ]
    Pfami PF01510. Amidase_2. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a Salmonella-specific region located between ampE and aroP genes."
      Cano D., Casadesus J., Garcia-del Portillo F.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SL1344.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SL1344.

    Entry informationi

    Entry nameiAMPD_SALTS
    AccessioniPrimary (citable) accession number: E1W818
    Secondary accession number(s): P30013, Q9L4I4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 8, 2011
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3