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E1W3A7 (APHA_HAEP3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Class B acid phosphatase

Short name=CBAP
EC=3.1.3.2
Gene names
Name:aphA
Ordered Locus Names:PARA_07400
OrganismHaemophilus parainfluenzae (strain T3T1) [Complete proteome] [HAMAP]
Taxonomic identifier862965 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the class B bacterial acid phosphatase family.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 235213Class B acid phosphatase
PRO_0000415226

Regions

Region135 – 1362Substrate binding By similarity

Sites

Active site671Nucleophile By similarity
Active site691Proton donor By similarity
Metal binding671Magnesium By similarity
Metal binding691Magnesium; via carbonyl oxygen By similarity
Metal binding1901Magnesium By similarity
Binding site1751Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
E1W3A7 [UniParc].

Last modified November 30, 2010. Version 1.
Checksum: 6F39B06DD55F912C

FASTA23526,222
        10         20         30         40         50         60 
MKNLLKLSAI AILAASAVST FASNKEPYTE QGTNAREMTE QKPIHWISVE QLKKELEGKA 

        70         80         90        100        110        120 
PINVSFDIDD TVLFSSPCFY HGQEKYSPGK NDYLKNQDFW NEVNAGCDQY SIPKQIAVDL 

       130        140        150        160        170        180 
INMHQARGDQ IYFITGRTAG DKDGVTPVLQ KAFNIKDMHP VEFMGGRKLP TKYNKTPGII 

       190        200        210        220        230 
EHKVSIHYGD SDDDILAAKE AGIRGIRLMR AANSTYQPMP TLGGYGEEVL INSNY 

« Hide

References

[1]"The genome sequence of Haemophilus parainfluenzae T3T1."
Crook D., Hood D., Moxon R., Parkhill J., Aslett M., Bentley S.D.
Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T3T1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FQ312002 Genomic DNA. Translation: CBW14847.1.
RefSeqYP_004822438.1. NC_015964.1.

3D structure databases

ProteinModelPortalE1W3A7.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBW14847; CBW14847; PARA_07400.
GeneID11116604.
KEGGhpr:PARA_07400.
PATRIC43050586. VBIHaePar168133_0738.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03788.

Enzyme and pathway databases

BioCycHPAR862965:GH07-756-MONOMER.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01672. AphA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAPHA_HAEP3
AccessionPrimary (citable) accession number: E1W3A7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 30, 2010
Last modified: April 16, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families