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E1W3A7

- APHA_HAEP3

UniProt

E1W3A7 - APHA_HAEP3

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Protein

Class B acid phosphatase

Gene

aphA

Organism
Haemophilus parainfluenzae (strain T3T1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671NucleophileBy similarity
Metal bindingi67 – 671MagnesiumBy similarity
Active sitei69 – 691Proton donorBy similarity
Metal bindingi69 – 691Magnesium; via carbonyl oxygenBy similarity
Binding sitei175 – 1751SubstrateBy similarity
Metal bindingi190 – 1901MagnesiumBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciHPAR862965:GH07-756-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseImportedBy similarity (EC:3.1.3.2By similarity)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:PARA_07400
OrganismiHaemophilus parainfluenzae (strain T3T1)
Taxonomic identifieri862965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000007052: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 235213Class B acid phosphataseSequence AnalysisPRO_0000415226Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliE1W3A7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1362Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

KOiK03788.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E1W3A7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNLLKLSAI AILAASAVST FASNKEPYTE QGTNAREMTE QKPIHWISVE
60 70 80 90 100
QLKKELEGKA PINVSFDIDD TVLFSSPCFY HGQEKYSPGK NDYLKNQDFW
110 120 130 140 150
NEVNAGCDQY SIPKQIAVDL INMHQARGDQ IYFITGRTAG DKDGVTPVLQ
160 170 180 190 200
KAFNIKDMHP VEFMGGRKLP TKYNKTPGII EHKVSIHYGD SDDDILAAKE
210 220 230
AGIRGIRLMR AANSTYQPMP TLGGYGEEVL INSNY
Length:235
Mass (Da):26,222
Last modified:November 30, 2010 - v1
Checksum:i6F39B06DD55F912C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FQ312002 Genomic DNA. Translation: CBW14847.1.
RefSeqiWP_014064597.1. NC_015964.1.
YP_004822438.1. NC_015964.1.

Genome annotation databases

EnsemblBacteriaiCBW14847; CBW14847; PARA_07400.
GeneIDi11116604.
KEGGihpr:PARA_07400.
PATRICi43050586. VBIHaePar168133_0738.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FQ312002 Genomic DNA. Translation: CBW14847.1 .
RefSeqi WP_014064597.1. NC_015964.1.
YP_004822438.1. NC_015964.1.

3D structure databases

ProteinModelPortali E1W3A7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CBW14847 ; CBW14847 ; PARA_07400 .
GeneIDi 11116604.
KEGGi hpr:PARA_07400.
PATRICi 43050586. VBIHaePar168133_0738.

Phylogenomic databases

KOi K03788.

Enzyme and pathway databases

BioCyci HPAR862965:GH07-756-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Haemophilus parainfluenzae T3T1."
    Crook D., Hood D., Moxon R., Parkhill J., Aslett M., Bentley S.D.
    Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T3T1.

Entry informationi

Entry nameiAPHA_HAEP3
AccessioniPrimary (citable) accession number: E1W3A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 30, 2010
Last modified: November 26, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3