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Protein

Class B acid phosphatase

Gene

aphA

Organism
Haemophilus parainfluenzae (strain T3T1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67NucleophileBy similarity1
Metal bindingi67MagnesiumBy similarity1
Active sitei69Proton donorBy similarity1
Metal bindingi69Magnesium; via carbonyl oxygenBy similarity1
Binding sitei175SubstrateBy similarity1
Metal bindingi190MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciHPAR862965:GH07-751-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseBy similarityImported (EC:3.1.3.2By similarity)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:PARA_07400
OrganismiHaemophilus parainfluenzae (strain T3T1)
Taxonomic identifieri862965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000007052 Componenti: Chromosome

Subcellular locationi

  • Periplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000041522623 – 235Class B acid phosphataseSequence analysisAdd BLAST213

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi862965.PARA_07400.

Structurei

3D structure databases

ProteinModelPortaliE1W3A7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 136Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
KOiK03788.
OMAiPEFWEKM.
OrthoDBiPOG091H06BK.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E1W3A7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNLLKLSAI AILAASAVST FASNKEPYTE QGTNAREMTE QKPIHWISVE
60 70 80 90 100
QLKKELEGKA PINVSFDIDD TVLFSSPCFY HGQEKYSPGK NDYLKNQDFW
110 120 130 140 150
NEVNAGCDQY SIPKQIAVDL INMHQARGDQ IYFITGRTAG DKDGVTPVLQ
160 170 180 190 200
KAFNIKDMHP VEFMGGRKLP TKYNKTPGII EHKVSIHYGD SDDDILAAKE
210 220 230
AGIRGIRLMR AANSTYQPMP TLGGYGEEVL INSNY
Length:235
Mass (Da):26,222
Last modified:November 30, 2010 - v1
Checksum:i6F39B06DD55F912C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FQ312002 Genomic DNA. Translation: CBW14847.1.
RefSeqiWP_014064597.1. NC_015964.1.

Genome annotation databases

EnsemblBacteriaiCBW14847; CBW14847; PARA_07400.
GeneIDi25057919.
KEGGihpr:PARA_07400.
PATRICi43050586. VBIHaePar168133_0738.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FQ312002 Genomic DNA. Translation: CBW14847.1.
RefSeqiWP_014064597.1. NC_015964.1.

3D structure databases

ProteinModelPortaliE1W3A7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi862965.PARA_07400.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBW14847; CBW14847; PARA_07400.
GeneIDi25057919.
KEGGihpr:PARA_07400.
PATRICi43050586. VBIHaePar168133_0738.

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
KOiK03788.
OMAiPEFWEKM.
OrthoDBiPOG091H06BK.

Enzyme and pathway databases

BioCyciHPAR862965:GH07-751-MONOMER.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPHA_HAEP3
AccessioniPrimary (citable) accession number: E1W3A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 30, 2010
Last modified: November 2, 2016
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.