E1W3A7 (APHA_HAEP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Class B acid phosphatase
Short name=CBAP
EC=3.1.3.2

Gene names

Name:	aphA
Ordered Locus Names:	PARA_07400

Organism

Haemophilus parainfluenzae (strain T3T1) [Complete proteome] [HAMAP]

Taxonomic identifier

862965 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus parainfluenzae

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class B bacterial acid phosphatase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular_function	acid phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 235	213	Class B acid phosphatase		PRO_0000415226
Regions
	Region	135 – 136	2	Substrate binding By similarity
Sites
	Active site	67	1	Nucleophile By similarity
	Active site	69	1	Proton donor By similarity
	Metal binding	67	1	Magnesium By similarity
	Metal binding	69	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	190	1	Magnesium By similarity
	Binding site	175	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	E1W3A7 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: 6F39B06DD55F912C	FASTA	235	26,222
	10 20 30 40 50 60 MKNLLKLSAI AILAASAVST FASNKEPYTE QGTNAREMTE QKPIHWISVE QLKKELEGKA 70 80 90 100 110 120 PINVSFDIDD TVLFSSPCFY HGQEKYSPGK NDYLKNQDFW NEVNAGCDQY SIPKQIAVDL 130 140 150 160 170 180 INMHQARGDQ IYFITGRTAG DKDGVTPVLQ KAFNIKDMHP VEFMGGRKLP TKYNKTPGII 190 200 210 220 230 EHKVSIHYGD SDDDILAAKE AGIRGIRLMR AANSTYQPMP TLGGYGEEVL INSNY

References

[1]	"The genome sequence of Haemophilus parainfluenzae T3T1." Crook D., Hood D., Moxon R., Parkhill J., Aslett M., Bentley S.D. Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T3T1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FQ312002 Genomic DNA. Translation: CBW14847.1.
RefSeq	YP_004822438.1. NC_015964.1.
3D structure databases
ProteinModelPortal	E1W3A7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBW14847; CBW14847; PARA_07400.
GeneID	11116604.
KEGG	hpr:PARA_07400.
PATRIC	43050586. VBIHaePar168133_0738.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K03788.
Enzyme and pathway databases
BioCyc	HPAR862965:GH07-707-MONOMER.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR005519. Acid_phosphat_B. IPR023214. HAD-like_dom. IPR010025. HAD-SF_ppase_IIIB_AphA. [Graphical view]
Pfam	PF03767. Acid_phosphat_B. 1 hit. [Graphical view]
PIRSF	PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01672. AphA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APHA_HAEP3

Accession

Primary (citable) accession number: E1W3A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 25, 2012
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program